AXIS-SHIELD

Axis-Shield will exhibit at the Experimental Biology meeting 2010 at the Anaheim Convention Center starting on Sunday April 25th. We are in booth 610. Please visit us to get the latest information regarding our Density Gradient Media.

Axis-Shield’s density gradient media – Nycodenz and OptiPrep can provide higher resolution of endosomal compartments than the traditional sucrose gradients. The new Axis-Shield Mini-Review No. 3 [2010] provides a summary of the technology as applied to both tissues and cultured cells. In addition to the routine pre-formed gradients, OptiPrep can also offer a self-generated mode that makes gradient formation simple and highly reproducible.

A companion Mini-Review – No. 2 [2010] – provides a reference list of the all the known papers reporting the use of both Nycodenz and OptiPrep in endocytosis analysis. The list is divided into tissue and cell type and, when merited by the number of references, also into functional study.

Detailed methodologies are given in a series of Axis-Shield Application Sheets that are listed in Mini-Review 2 [2010].

Mini-Reviews 2 2010  and 3 2010 are available by clicking on "Brochures" in the main menu

One of the focuses of tissue engineering and regenerative medicine research is the efficient in vitro growth of cells from biopsy material on a plastic substratum, as a prelude to its eventual use clinically. A recent paper by Bruce et al [1] reported on the culture of urothelial cells from non-neurogenic and neurogenic human bladder tissue. The publication described the suspension of the cells in a 12% (w/v) iodixanol solution, overlaid with a small layer of culture medium. During centrifugation this allowed the viable epithelial cells to float to the surface while non viable cells, debris and erythrocytes sediment. The absence of an interface at which viable cells and non-viable cells might aggregate together is a critical feature of this system. The authors reported that the iodixanol “clean-up” increased the percentage of attached cells 24 h after plating from 27% to 84% and that multiple large colonies of cells were observed with the purified cells.

¨ The well-established low endotoxin levels in OptiPrep and the clinical acceptability of iodixanol as an X-ray imaging agent underline the importance of applications in tissue engineering and regenerative medicine research

Bruce, A.T., Sangha, N., Richmond, A., Johnson, K., Jones, S., Spencer, T. and Ludlow, J.W. (2010) Use of iodixanol self-generated density gradients to enrich for viable urothelial cells from non-neurogenic and neurogenic bladder tissue Tissue Eng., Part C Methods 16, 33-40

Axis-Shield Application Sheet C13 provides detailed methodological options for viable cell enrichment and can be accessed by clicking on "Isolation of mammalian cells" on the front page.

Axis-Shield will exhibit at the Neuroscience meeting in Chicago, October 18-21. Our booth number is 543.

All Application Sheets have now been updated and new ones have been added. The new section is called OptiPrep/Nycodenz Application Sheets due to the fact that also Nycodenz applications have been added. All Application Sheets can be reached from the home page.

We have also updated all our brochures, which can be found on the main menu.

We are pleased to announce that Cosmo Bio Co, Ltd is our new distributor in Japan from first of April 2008. Cosmo Bio has taken over the distributionship from Daiichi Pure Chemicals which has decided to concentrate on other products.  

All details on Cosmo Bio can be found in our Distributor list.

All application sheets in the C-series have now been updated. We have also added a few new applications based on Nycodenz. To find all the different applications, click on Methodology on the main menu.

Today we have the pleasure of launching  another OptiPrep Application Sheet:

C40 Isolation of mononuclear cells from tissues.

To find the new methods click on "Methodology" in the main menu

We have today launched two new OptiPrepTM Application sheets.

C43 Isolation of mononuclear cells from rat, mouse and rabbit blood on a density barrier, is a replacement for the product NycoPrepTM 1.077A which was withdrawn from the market earlier this year.

C45 Isolation of polymorphonuclear leukocytes (granulocytes) from rat, mouse and rabbit blood, is an addition to our product Polymorphprep, which is used for the isolation of polymorphonuclear cells from human blood.

Both application sheets can be found by clicking on "OptiPrep Application Sheets" in the main menu.

We have also launced a Polymorphprep Applications Sheet, which can be found under the heading "Products"

We have the pleasure of introducing to you our new catalogue, Applications and Products 2007/2008. The catalogue contain information on how to make gradients and gradient solutions, harvesting of gradients and general information on different applications using our products.

The new catalog is available in a printed version or a CD version. It could also be downloaded on this site by clicking on Catalogue in the main toolbar.