HiFi DNA Polymerase: Achieving Unparalleled Fidelity in Molecular Amplification
Discover the pinnacle of PCR accuracy with HiFi DNA Polymerase, a revolutionary enzyme that delivers exceptional fidelity, remarkable efficiency, and broad compatibility. This groundbreaking polymerase empowers researchers to unravel the complexities of DNA with unprecedented precision and confidence.
HiFi DNA Polymerase: The Benchmark for Unparalleled Fidelity in PCR
Introduction
HiFi DNA Polymerase, a groundbreaking enzyme derived from a proprietary thermophilic bacterium, stands as the pinnacle of PCR accuracy. This revolutionary polymerase redefines the standards of fidelity, efficiency, and compatibility, empowering researchers to explore the complexities of DNA with unprecedented precision and confidence.
Product Features
- Unparalleled Fidelity: HiFi DNA Polymerase introduces a new era of PCR fidelity, boasting an error rate approximately 280X lower than Taq polymerase. This unparalleled accuracy ensures the integrity of amplified DNA sequences, minimizing the risk of introducing errors that could alter downstream applications.
- Remarkable Efficiency: HiFi DNA Polymerase excels in both speed and processivity, enabling efficient amplification of even long and complex DNA templates. Its ability to rapidly synthesize DNA strands without stalling or misincorporating nucleotides ensures the generation of high-quality PCR products.
- Broad Compatibility: HiFi DNA Polymerase seamlessly integrates with a diverse range of PCR buffers and reaction conditions, offering unmatched versatility for a wide spectrum of applications. Its ability to adapt to various experimental setups without compromising performance makes it the preferred choice for a variety of molecular biology techniques.
Product Applications
HiFi DNA Polymerase's exceptional properties make it an indispensable tool for a wide range of molecular biology applications, including:
- High-Fidelity PCR: HiFi DNA Polymerase serves as the gold standard for high-fidelity PCR, enabling the amplification of DNA with unmatched accuracy for various downstream applications, such as cloning, mutagenesis, and NGS library preparation.
- Long PCR: HiFi DNA Polymerase's exceptional processivity makes it the ideal choice for amplifying long DNA fragments, up to 20 kb, without compromising fidelity. This capability proves particularly valuable for applications requiring the amplification of large genomic regions or full-length genes.
- GC-Rich PCR: HiFi DNA Polymerase effectively amplifies GC-rich DNA sequences, overcoming the challenges often encountered with conventional polymerases. Its ability to overcome GC-rich regions without stalling or misincorporating nucleotides ensures the accurate amplification of challenging DNA templates.
- NGS Library Preparation: HiFi DNA Polymerase is the preferred enzyme for generating high-quality NGS libraries, ensuring the integrity and accuracy of sequencing data. Its ability to minimize errors during amplification guarantees the production of high-fidelity libraries, minimizing the risk of introducing sequencing artifacts.
Product Benefits
HiFi DNA Polymerase's unparalleled fidelity, remarkable efficiency, and broad compatibility translate into a multitude of benefits for researchers:
- Enhanced Accuracy: HiFi DNA Polymerase's exceptional fidelity minimizes the risk of introducing errors during amplification, ensuring the accuracy of downstream applications.
- Improved Efficiency: HiFi DNA Polymerase's rapid synthesis rates and processivity lead to faster and more efficient PCR reactions, reducing experimental time and increasing throughput.
- Expanded Versatility: HiFi DNA Polymerase's broad compatibility with various buffers and reaction conditions expands its applicability to a wide range of molecular biology techniques.
Overall, HiFi DNA Polymerase stands as a transformative tool for molecular biology research, redefining the boundaries of PCR fidelity, efficiency, and compatibility. By empowering researchers to amplify DNA with unprecedented precision and confidence, HiFi DNA Polymerase unlocks new avenues for exploring the intricate world of genetics and genomics.
HiFi DNA Polymerase: Elevating PCR Fidelity to New Heights
Product Introduction
HiFi DNA Polymerase, a revolutionary enzyme derived from Pfu DNA Polymerase, marks a significant leap forward in PCR fidelity. Compared to its predecessor, HiFi DNA Polymerase exhibits remarkable enhancements in performance and versatility, enabling researchers to tackle complex PCR reactions with unprecedented accuracy and efficiency.
Product Features
- Exceptional Purity: HiFi DNA Polymerase boasts a purity of over 95%, ensuring minimal interference from impurities and maximizing reaction efficiency.
- Unparalleled Fidelity: HiFi DNA Polymerase surpasses the fidelity of Taq polymerase by over 100-fold, minimizing the introduction of errors during amplification and ensuring the integrity of DNA sequences.
- Remarkable Efficiency: HiFi DNA Polymerase excels in amplifying even challenging targets, including high GC content DNA, poorly purified DNA, and long fragment sequences. Its ability to rapidly synthesize DNA strands without stalling or misincorporating nucleotides ensures the generation of high-quality PCR products.
- Convenient Pre-Mixed Solutions: HiFi DNA Polymerase is available in both pre-mixed solutions with and without loading dye, offering convenience and flexibility for various experimental setups.
Product Applications
HiFi DNA Polymerase's exceptional properties make it an indispensable tool for a diverse range of molecular biology applications, including:
- Long Fragment PCR Amplification: HiFi DNA Polymerase seamlessly amplifies long DNA fragments, up to 20 kb, without compromising fidelity or efficiency. This capability proves invaluable for applications requiring the amplification of large genomic regions or full-length genes.
- High GC Template Amplification: HiFi DNA Polymerase effectively overcomes the challenges of amplifying GC-rich DNA sequences, often a hurdle for conventional polymerases. Its ability to synthesize DNA accurately through GC-rich regions ensures the successful amplification of challenging DNA templates.
- DNA Fragment Cloning: HiFi DNA Polymerase's high fidelity and processivity make it the preferred enzyme for cloning DNA fragments. Its ability to generate accurate and high-quality PCR products simplifies the cloning process and ensures the integrity of cloned sequences.
Product Data
Detailed product data, including specifications, performance characteristics, and storage conditions, is available upon request.
Line 1-4: High GC gDNA 1K, 0.7K, 0.5K, 0.4K (GC content 60-80%).
Line 5-8: λ DNA 1K, 4K, 6K, 8K.
HiFi DNA Polymerase: Empowering Researchers to Explore the Intricate World of DNA with Precision and Confidence
With its exceptional fidelity, remarkable efficiency, and broad applicability, HiFi DNA Polymerase redefines the standards of PCR performance. By empowering researchers to amplify DNA with unprecedented accuracy and confidence, HiFi DNA Polymerase opens doors to a new era of molecular biology research, enabling deeper insights into the complexities of genetics and genomics.
Product Manual
| Property | Value |
|---|---|
| Product Name | HiFi DNA Polymerase |
| Source | Recombinant expression in E. coli |
| Catalog Number | CSB-DEM042 |
| Physical Form | Liquid |
| Enzyme Activity | 1 U/μL |
| Storage Conditions | -20 ±5°C |
| Storage Buffer | 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween20, 0.5% NP-40, 50% Glycerol |
| Activity Definition | Within 30 minutes at 72°C, 1 unit (U) of the enzyme is required to incorporate 10 nmol of deoxynucleotides into acid-insoluble material. |
| Quality Control | No detectable nuclease and endonuclease activities |
| Shelf Life | 24 months |
HiFi DNA Polymerase: Transcending the Boundaries of PCR Fidelity and Efficiency
HiFi DNA Polymerase, a groundbreaking enzyme engineered from modified Pfu DNA Polymerase, revolutionizes the landscape of PCR fidelity and efficiency. This innovative polymerase surpasses the limitations of its predecessor, Pfu DNA Polymerase, delivering remarkable enhancements in performance and versatility.
Unparalleled Fidelity: HiFi DNA Polymerase sets a new benchmark in PCR fidelity, achieving an error rate over 100-fold lower than Taq polymerase. This exceptional accuracy ensures the integrity of amplified DNA sequences, minimizing the introduction of errors that could hinder downstream applications.
Exceptional Efficiency: HiFi DNA Polymerase excels in amplifying even the most challenging DNA targets, including high GC content DNA, poorly purified DNA, and long fragment sequences. Its remarkable processivity and rapid synthesis rates enable the efficient generation of high-quality PCR products.
Optimized Buffer System: HiFi DNA Polymerase comes equipped with an optimized enzyme buffer containing PCR-enhancing components. This synergistic combination grants the enzyme exceptional amplification efficiency and broad template adaptability, making it suitable for amplifying even the most complex DNA templates.
Blunt-End Amplification Products: HiFi DNA Polymerase generates PCR products with blunt ends, simplifying downstream applications such as cloning and ligation. This feature eliminates the need for additional enzymatic steps, saving time and enhancing experimental efficiency.
Applications:
- Long Fragment PCR Amplification: HiFi DNA Polymerase seamlessly amplifies long DNA fragments, up to 20 kb, without compromising fidelity or efficiency. This capability proves invaluable for applications requiring the amplification of large genomic regions or full-length genes.
- High GC Template Amplification: HiFi DNA Polymerase effectively overcomes the challenges of amplifying GC-rich DNA sequences, often a hurdle for conventional polymerases. Its ability to synthesize DNA accurately through GC-rich regions ensures the successful amplification of challenging DNA templates.
- DNA Fragment Cloning: HiFi DNA Polymerase's high fidelity and processivity make it the preferred enzyme for cloning DNA fragments. Its ability to generate accurate and high-quality PCR products simplifies the cloning process and ensures the integrity of cloned sequences.
HiFi DNA Polymerase: Unleashing the Power of Precision PCR
HiFi DNA Polymerase marks a significant leap forward in PCR technology, empowering researchers to explore the intricacies of DNA with unprecedented precision and confidence. By combining exceptional fidelity, remarkable efficiency, and broad applicability, HiFi DNA Polymerase opens doors to new avenues of research and unlocks the hidden secrets of the genetic code.