KOD DNA Polymerase: A High-Fidelity, Thermostable Enzyme for PCR Applications
KOD DNA Polymerase is a highly accurate and fast DNA polymerase derived from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1. It exhibits exceptional amplification efficiency and fidelity, making it an indispensable tool for a wide range of molecular biology applications. With an error rate approximately 1.3 x 10^-6 errors per base pair, KOD DNA Polymerase stands as one of the most accurate DNA polymerases available, ensuring the integrity of amplified sequences and making it ideal for sensitive applications such as cloning, mutagenesis, and gene expression studies. Additionally, its remarkable amplification speed of up to 2 kb/min streamlines PCR protocols and enhances research productivity.
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Product Introduction
KOD DNA Polymerase is a high-fidelity, thermostable DNA polymerase isolated from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1. It is one of the most accurate DNA polymerases available, with an error rate of approximately 1.3 x 10^-6 errors per base pair. This makes it ideal for applications where accurate DNA amplification is critical, such as cloning, mutagenesis, and gene expression studies. KOD DNA Polymerase is also very fast, with an amplification rate of up to 2 kb/min. This makes it a good choice for high-throughput PCR applications.
Key Features
- High fidelity: KOD DNA Polymerase has an error rate of approximately 1.3 x 10^-6 errors per base pair, making it one of the most accurate DNA polymerases available.
- Fast: KOD DNA Polymerase has an amplification rate of up to 2 kb/min, making it a good choice for high-throughput PCR applications.
- Thermostable: KOD DNA Polymerase is stable at high temperatures, with an optimal reaction temperature of 75°C. This makes it suitable for use in PCR applications.
- 3'→5' exonuclease activity: KOD DNA Polymerase has proofreading activity, which allows it to remove incorrectly incorporated nucleotides and thus reduce the error rate of DNA synthesis.
Applications
KOD DNA Polymerase is widely used in a variety of molecular biology applications, including:
- High-fidelity PCR: KOD DNA Polymerase is commonly used for high-fidelity PCR applications, where accurate DNA synthesis is critical.
- Site-directed mutagenesis: KOD DNA Polymerase is used in site-directed mutagenesis, a technique used to introduce specific mutations into DNA sequences.
- DNA sequencing: KOD DNA Polymerase is used in some DNA sequencing methods, such as Sanger sequencing.
- Cloning: KOD DNA Polymerase is used for cloning, a technique used to generate multiple copies of a DNA sequence.
- Gene expression studies: KOD DNA Polymerase is used in gene expression studies, such as RT-PCR, to quantify the expression of genes.
- PCR Cloning: KOD DNA Polymerase is the preferred enzyme for PCR cloning applications due to its high fidelity and blunt-end amplification capabilities.
- Mutation Repair: KOD DNA Polymerase's proofreading activity makes it an ideal choice for mutation repair applications, enabling the correction of DNA sequence errors.
- Sequencing Reactions: KOD DNA Polymerase is used in some DNA sequencing methods, such as Sanger sequencing, due to its high fidelity and ability to amplify long DNA fragments.
- Complex Template Amplification: KOD DNA Polymerase's ability to amplify complex DNA templates makes it suitable for applications such as GC-rich PCR and amplification of repetitive sequences.
Advantages of KOD DNA Polymerase
KOD DNA Polymerase offers several advantages over other DNA polymerases, including:
- High fidelity: KOD DNA Polymerase has one of the lowest error rates of any DNA polymerase, making it ideal for applications where accurate DNA synthesis is critical.
- Fast: KOD DNA Polymerase is one of the fastest DNA polymerases available, making it a good choice for high-throughput PCR applications.
- Thermostability: KOD DNA Polymerase is stable at high temperatures, making it suitable for use in PCR applications.
- Proofreading activity: KOD DNA Polymerase has proofreading activity, which further reduces the error rate of DNA synthesis.
Disadvantages of KOD DNA Polymerase
KOD DNA Polymerase also has some disadvantages, including:
- High cost: KOD DNA Polymerase is more expensive than some other DNA polymerases, such as Taq DNA Polymerase.
- Poor processivity: KOD DNA Polymerase has a lower processivity than some other DNA polymerases, meaning that it falls off of the DNA template more frequently.
Overall, KOD DNA Polymerase is a powerful tool for molecular biology research. Its high fidelity, fast amplification rate, and thermostability make it ideal for a variety of applications, including high-fidelity PCR, site-directed mutagenesis, DNA sequencing, cloning, and gene expression studies.
| Property | Value |
|---|---|
| Product Name | KOD DNA Polymerase |
| Source | Recombinantly expressed in Escherichia coli |
| Catalog Number | CSB-DEM034 |
| Physical Form | Liquid |
| Enzyme Activity | 5 U/μL |
| Storage Conditions | -20 ±5°C |
| Storage Buffer | 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween20, 0.5% NP-40, 50% Glycerol |
| Activity Definition | 1 unit (U) of the enzyme is defined as the amount required to incorporate 10 nmol of deoxynucleotide into acid-insoluble material in 30 min at 72°C. |
| Quality Control | No detectable 3'-5' exonuclease and endonuclease activities |
| Shelf Life | 24 months |
| No. | Component Name | Specifications |
|---|---|---|
| 1 | 5× KOD buffer | 0.6 mL, 3 mL, 6 mL |
| 2 | KOD DNA Polymerase | 100 U, 500 U, 1000 U |
KOD DNA Polymerase: Unleash Precision and Efficiency in PCR
Introducing KOD DNA Polymerase, an exceptional enzyme derived from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1, specifically designed to revolutionize your PCR workflow. Through recombinant expression in Escherichia coli, KOD DNA Polymerase delivers exceptional amplification efficiency, remarkable fidelity, and unparalleled speed, making it an indispensable tool for your molecular biology endeavors.
Unmatched Fidelity for Error-Free PCR
KOD DNA Polymerase stands out from the crowd with its exceptional 3'-5' exonuclease activity, also known as proofreading activity. This unique feature enables the enzyme to meticulously proofread newly synthesized DNA, correcting any misincorporations and dramatically reducing error rates. With KOD DNA Polymerase, you can bid farewell to PCR-induced mutations and confidently rely on the accuracy of your amplified DNA sequences.
Amplification Efficiency: Every Target Molecule Counts
KOD DNA Polymerase epitomizes efficiency, seamlessly amplifying target DNA sequences of up to 6 kb in length. Its remarkable speed of 1 kb/10s ensures rapid PCR reactions, saving you valuable time and effort. Whether you're amplifying short or long DNA fragments, KOD DNA Polymerase delivers consistent, high-yield amplifications, ensuring that every target molecule is captured and amplified with precision.
Blunt-Ended Products: Seamless Cloning and Sequencing
KOD DNA Polymerase generates blunt-ended DNA products, eliminating the need for additional enzymatic modification prior to downstream applications. This feature streamlines your cloning and sequencing workflows, saving you time and minimizing potential errors. With blunt-ended PCR products, you can seamlessly ligate DNA fragments into vectors and directly proceed with sequencing without the hassle of overhang modification.
KOD DNA Polymerase: Your Partner in PCR Excellence
KOD DNA Polymerase is the epitome of precision, efficiency, and convenience in PCR. Its exceptional fidelity ensures error-free amplification, while its remarkable speed and high yield empower you to achieve optimal results in every PCR reaction. With KOD DNA Polymerase, you can confidently tackle a wide range of molecular biology applications, from gene cloning to mutation analysis, with the assurance of unsurpassed accuracy and efficiency. Embrace the power of KOD DNA Polymerase and elevate your PCR experiments to new heights of precision and efficiency.