WSE-1165 Mini-Slab: A Versatile Tool for Protein Electrophoresis

The WSE-1165 Mini-Slab stands out as a compact and efficient gel electrophoresis system, tailored for the rapid separation of proteins in diverse applications. This versatile tool is not only easy to use but also encompasses a range of features and benefits that make it an ideal choice for laboratories of all sizes.

Key Applications:

1. SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis):
   • Separates proteins based on their molecular weight.
2. Native PAGE:
   • Separates proteins according to their native charge and size.
3. 2D PAGE:
   • Combines isoelectric focusing (IEF) and SDS-PAGE for high-resolution protein separation.

Features and Benefits:

The WSE-1165 Mini-Slab incorporates key features that enhance its usability and effectiveness in protein electrophoresis:

• Compact and Portable Design:
  • Its compact and lightweight design facilitates easy transport and versatile use in various settings.
• Durable Construction:
  • Crafted from high-quality materials, the mini-slab ensures durability through repeated use.
• Easy-to-Use Design:
  • The user-friendly design makes assembly and operation straightforward, even for beginners.
• Compatibility:
  • The mini-slab is compatible with a wide range of buffers and gels, making it adaptable to various protein analysis applications.
• Cost-Effectiveness:
  • Providing a cost-effective solution for protein electrophoresis, it is accessible for laboratories with diverse budgets.

Specifications:

• Gel Dimensions: 8x9cm
• Gel Thickness: 0.75mm
• Sample Loading Capacity: Up to 20 samples per gel
• Running Time: Approximately 20-30 minutes

Applications:

The WSE-1165 Mini-Slab finds utility in an array of protein analysis applications, including:

• Purity Analysis: Assessing the purity of protein samples.
• Molecular Weight Determination: Establishing the molecular weight of proteins.
• Protein Identification: Identifying proteins based on their migration pattern.
• Protein Quantification: Measuring the amount of protein in a sample.

• Protein-Protein Interaction Studies: Investigating interactions between proteins.

How to Use:

1. Gel Preparation:
   • Mix acrylamide, bisacrylamide, and Tris-borate-EDTA (TBE) buffer solutions.
   • Pour the gel into the mold and allow polymerization.
2. Sample Loading:
   • Load protein samples into the gel wells.
3. Electrophoresis:
   • Fill electrolyte reservoirs with TBE buffer.
   • Apply an electric voltage for 30-60 minutes until proteins migrate.
4. Visualization:
   • Stop electrophoresis and remove the gel.
   • Visualize proteins using staining methods like Coomassie blue or silver nitrate.

In conclusion, the WSE-1165 Mini-Slab emerges as a valuable and versatile solution for protein electrophoresis. Its compact design, ease of use, and compatibility with various applications make it an optimal choice for laboratories seeking efficient and cost-effective protein analysis.

Objective and Usage

• SDS-PAGE (SDS-Polyacrylamide Gel Electrophoresis)
• Native-PAGE (Non-denatured Polyacrylamide Gel Electrophoresis)
• Protein separation, identification, purity confirmation, and molecular weight estimation, etc.
• Assess the purified or expressed protein levels
• Electrophoresis of DNA or confirmation of PCR products
• Screen your target protein expression
• Protein or nucleic acid electrophoresis for entry-level experiments and student training
• Determination of enzyme types

Features

• Mini PAGE system with a built-in power supply
• High mode
• Reduction of working space
• Automatic polarity switching function
• Quick sealing method
• Prevention of buffer solution leakage with flexible material in a special wave shape; electrophoresis plate and gel are securely retained
• Two-sided constant temperature using a lower buffer solution for reducing the thermostatic smiling
• Compatible caster: AE-6401 1 mm Dual Mini Gel Cast (Holder for Hand-Cast Gel), WSE-1190 Multi Mini-Slab Gel Cast (Holder for PAGEL)
• Compatible precast gel (Holder for PAGEL): e-PAGEL, e-PAGEL HR

Specifications

WSE-1165 Mini Slab (2322198) without built-in Power Supply

Specifications

• Gel size: 8x9cm
• Number of wells: 12
• Voltage: 50-300V
• Current: 10-50mA
• Dimensions: 150x100x100mm
• Weight: 1kg

WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply

Specifications

• Gel size: 8x9cm
• Number of wells: 12
• Voltage: 50-300V
• Current: 10-50mA
• Power supply: DC 50V/5A
• Dimensions: 250x150x150mm
• Weight: 2kg

Key differences

• The WSE-1165 Mini Slab (2322198) without built-in Power Supply requires a separate power supply to operate, while the WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply has a built-in power supply and does not require a separate power supply.
• The WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply is larger and heavier than the WSE-1165 Mini Slab (2322198) without built-in Power Supply.

Which model should you choose?

If you already have a power supply that you can use with the WSE-1165 Mini Slab (2322198) without built-in Power Supply, then there is no need to purchase the WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply. However, if you do not have a power supply, then the WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply is a convenient option.

If you have limited bench space, then the WSE-1165 Mini Slab (2322198) without built-in Power Supply is a better choice, as it is smaller and lighter than the WSE-1165 Mini Slab (2321655/2321659) with built-in Power Supply.

Gel Casting Kit (for hand-made gel)

The Gel Casting Kit (for hand-made gel) : WSE-1190 Multi Mini Slab Gel Cast (2393031) and AE-6401 1mm Dual Mini Gel Cast（2393010） are two different gel casting kits that can be used to create handmade polyacrylamide gels for SDS-PAGE electrophoresis.

WSE-1190 Multi Mini Slab Gel Cast (2393031)

This kit can be used to cast up to four mini slab gels at a time, each with a gel size of 90mm x 83mm and a thickness of 1mm. The kit includes all of the necessary components for gel casting, including:

• Two glass plates (90mm x 102mm)
• Two spacer plates (1mm thick)
• Two clamping bars
• Four sealing gaskets
• Two combs (12-well)

AE-6401 1mm Dual Mini Gel Cast（2393010）

This kit can be used to cast two mini slab gels at a time, each with a gel size of 90mm x 80mm and a thickness of 1mm. The kit includes all of the necessary components for gel casting, including:

• Two glass plates (90mm x 102mm)
• Two spacer plates (1mm thick)
• Two clamping bars
• Two sealing gaskets
• Two combs (12-well)

Both of these kits are easy to use and produce high-quality gels. The main difference between the two kits is that the WSE-1190 Multi Mini Slab Gel Cast (2393031) can be used to cast more gels at a time.

Which kit should you choose?

If you need to cast multiple gels at a time, then the WSE-1190 Multi Mini Slab Gel Cast (2393031) is the better choice. However, if you only need to cast a few gels at a time, then the AE-6401 1mm Dual Mini Gel Cast（2393010） is a good option.

Here is a table that summarizes the key differences between the two kits: