AXIS-SHIELD Density Gradient Media
-a brand of Alere Technologies AS, Oslo, Norway
OPTIPREP APPLICATION SHEET INDEX
EUKARYOTIC AND PROKARYOTIC CELLS
♦ The Index is divided into two sections:
A. General methods for preparing and analysing gradients
B. An alphabetical cell type index. Commonly isolated cells such as mononuclear
cells are often further categorized into species or tissue source. If a cell type is not
in this index, it may be necessary to develop a customized method: in Section A
see “Fractionation of a mixed population of cells” for some guidance.
♦ Click on the relevant [APPLICATION SHEET] for a detailed protocol. In some cases
more than one Application Sheet for a specific cell type may be provided if different
practical strategies are available.
A. GENERAL METHODS
Preparation of gradient solutions [APPLICATION SHEET C01]
Preparation of gradients [APPLICATION SHEET C02]
Fractionation of a mixed population of cells [APPLICATION SHEET C14]
Analysis of gradients [APPLICATION SHEET C49]
B. ALPHABETICAL CELL INDEX
Alveolar cells (see “Pulmonary cells”)
Apoptosis analysis [APPLICATION SHEET C32]
Bacteria
Anaplasma phagocytophilum [APPLICATION SHEET C50]
Chlamydophila abortus [APPLICATION SHEET C50]
Cyanobacteria [APPLICATION SHEET C50]
Listeria monocytogenes [APPLICATION SHEET C50]
Obligate intracellular bacteria [APPLICATION SHEET C50]
Piscirickettsia [APPLICATION SHEET C50]
Rickettsia typhi [APPLICATION SHEET C50]
Spore protoplast density determination [APPLICATION SHEET C50]
Soil, clinical specimens and food, from [APPLICATION SHEET C39]
Cells in suspension, maintenance of [APPLICATION SHEET C38]
Cryptosporidium (see “Protozoa”)
Cyclospora (see “Protozoa”)
Dendritic cells
Barrier flotation [APPLICATION SHEET C20]
Barrier sedimentation [APPLICATION SHEET C46]
Mixer flotation [APPLICATION SHEET C21]
Enterocyozoon bieneusi (see “Protozoa”)
Erythrocytes (normal and sickle cells) [APPLICATION SHEET C35]
Erythrocytes, removal from blood and bone marrow [APPLICATION SHEET C35]
Foam cells [APPLICATION SHEET C46]
Gastric cells
Parietal cells [APPLICATION SHEET C28]
ECL cells [APPLICATION SHEET C31]
Granulocytes (see “Polymorphonuclear leukocytes”)
Hepatic cells
Kupffer cells [APPLICATION SHEET C47]
Non-parenchymal cells [APPLICATION SHEET C24]
Stellate cells [APPLICATION SHEET C33]
Kidney cells (see “Renal”)
Langerhans cells (see “Dendritic cells”)
Lymphocytes
Blood and tissues, from (see “Mononuclear cells”)
Macrophages [APPLICATION SHEET C45]
Mattesia orzaephili (see “Protozoa”)
Monocytes (human)
Leukocyte-rich plasma
Barrier sedimentation [APPLICATION SHEET C51]
Flotation [APPLICATION SHEET C09]
Whole blood, from [APPLICATION SHEET C10]
Mononuclear cells
Bone marrow [APPLICATION SHEET C40]
Equine peripheral blood [APPLICATION SHEET C08]
Human peripheral blood
Barrier flotation [APPLICATION SHEET C05]
Barrier sedimentation [APPLICATION SHEET C03]
Mixer flotation [APPLICATION SHEET C04]
Intestine [APPLICATION SHEET C40]
Liver [APPLICATION SHEET C40]
Mouse blood
Barrier sedimentation [APPLICATION SHEET C43]
Mixer flotation [APPLICATION SHEET C07]
Non-human primate peripheral blood [APPLICATION SHEET C30]
Peritoneal exudates [APPLICATION SHEET C19]
Rabbit blood [APPLICATION SHEET C43]
Rat blood
Barrier sedimentation [APPLICATION SHEET C43]
Mixer flotation [APPLICATION SHEET C06]
Ruminant peripheral blood [APPLICATION SHEET C08]
Spinal cord [APPLICATION SHEET C40]
Spleen [APPLICATION SHEET C40]
Neural cells
Inflammatory cells (spinal cord injury) [APPLICATION SHEET C36]
Microglial cells [APPLICATION SHEET C36]
Motoneurons (brain, various sites) [APPLICATION SHEET C29]
Motoneurons (spinal cord) [APPLICATION SHEET C22]
Oligodendrocytes (see “Microglial cells”)
Neutrophils
see “Polymorphonuclear leukocytes”
Pancreatic islets [APPLICATION SHEET C15]
Pancreatic stellate cells [APPLICATION SHEET C33]
Plant protoplasts [APPLICATION SHEET C18]
Platelets (human) [APPLICATION SHEET C12]
Polymorphonuclear leukocytes
Human peripheral blood [APPLICATION SHEET C11]
Mouse blood [APPLICATION SHEET C44]
Non-human primate peripheral blood (see “Human
peripheral blood”)
Peritoneal exudates [APPLICATION SHEET C19]
Rat blood (see “ Mouse blood”)
Rabbit blood (see “ Mouse blood”)
Spinal injury, quantitative assessment in [APPLICATION SHEET C36]
Progenitor cells (bone marrow and other tissues)
Barrier sedimentation [APPLICATION SHEET C23]
Protozoa
Cryptosporidium [APPLICATION SHEET C27]
Cyclospora [APPLICATION SHEET C27]
Enterocytozoon bieneusi [APPLICATION SHEET C27]
Mattesia orzaephili [APPLICATION SHEET C27]
Plasmodium [APPLICATION SHEET C41]
Sarcocystis neurona [APPLICATION SHEET C27]
Toxoplasma
Purification from cell cultures [APPLICATION SHEET C48]
Separation of sporocysts and oocyst walls [APPLICATION SHEET C34]
Pulmonary cells
Endothelial cells [APPLICATION SHEET C25]
Epithelial cells (Type I) [APPLICATION SHEET C25]
Epithelial cells (Type II) [APPLICATION SHEET C26]
Lymphoid cells [APPLICATION SHEET C26]
Macrophages [APPLICATION SHEET C25]
Myeloid cells [APPLICATION SHEET C26]
Renal cells
Interstitial and thin loop of Henlé cells [APPLICATION SHEET C42]
Proximal tubule [APPLICATION SHEET C42]
Reticulocytes [APPLICATION SHEET C35]
Sarcocystis neurona (see “Protozoa”)
Sea urchin coelomocytes [APPLICATION SHEET C37]
Sickle cells (see “Erythrocytes”)
Sperm cells
Bovine [APPLICATION SHEET C16]
Equine [APPLICATION SHEET C16]
Elephant [APPLICATION SHEET C16]
Gazelle [APPLICATION SHEET C16]
Human [APPLICATION SHEET C17]
Mouse [APPLICATION SHEET C16]
Porcine [APPLICATION SHEET C16]
Turkey/rooster [APPLICATION SHEET C16]
Spleen
Dendritic cells (see “Dendritic cells”)
Mononuclear cells (see “Mononuclear cells”)
Splenocytes (see “Mononuclear cells”)
Stem cells (see “Progenitor cells”)
Thymus
Dendritic cells (see “Dendritic cells”)
Thrombocytes (see “Platelets”)
Viable/non-viable cells [APPLICATION SHEET C13]
September 2016
Latest news
Axis-Shield will exhibit at the ASCB meeting in San Francisco December 3-7. You will find us in booth 418
We have recently posted a new package insert for Polymorphprep (see Products in the main menu). In addition to a new figure, we have added a new paragraph; Important practical and sample notes.
We have decided to discontinue the product NycoPrep 1.077, 4x250 ml. We still have lot no. 12GLS12 in stock, with an extended expiry date of November 2017.