Axis-Shield Density Gradient Media

  -a brand of Alere Technologies AS, Oslo, Norway

OPTIPREP APPLICATION SHEET INDEX

EUKARYOTIC AND PROKARYOTIC CELLS


♦ The Index is divided into two sections:


A. General methods for preparing and analysing gradients

B. An alphabetical cell type index. Commonly isolated cells such as mononuclear

    cells are often further categorized into species or tissue source. If a cell type is not

    in this index, it may be necessary to develop a customized method: in Section A

    see “Fractionation of a mixed population of cells” for some guidance.

 

♦ Click on the relevant [APPLICATION SHEET] for a detailed protocol. In some cases

   more than one Application Sheet for a specific cell type may be provided if different

   practical strategies are available.


A. GENERAL METHODS


Preparation of gradient solutions                                                           [APPLICATION SHEET C01]


Preparation of gradients                                                                        [APPLICATION SHEET C02] 


Fractionation of a mixed population of cells                                           [APPLICATION SHEET C14]


Analysis of gradients                                                                              [APPLICATION SHEET C49]


B. ALPHABETICAL CELL INDEX


Alveolar cells (see “Pulmonary cells”)


Apoptosis analysis                                                                              [APPLICATION SHEET C32]


Bacteria

Anaplasma phagocytophilum                                                                 [APPLICATION SHEET C50]


Chlamydophila abortus                                                                           [APPLICATION SHEET C50]


Cyanobacteria                                                                                        [APPLICATION SHEET C50]

 

Listeria monocytogenes                                                                         [APPLICATION SHEET C50]


Obligate intracellular bacteria                                                                [APPLICATION SHEET C50]


Piscirickettsia                                                                                         [APPLICATION SHEET C50]


Rickettsia typhi                                                                                       [APPLICATION SHEET C50]


Spore protoplast density determination                                                 [APPLICATION SHEET C50]


Soil, clinical specimens and food, from                                                  [APPLICATION SHEET C39]


Cells in suspension, maintenance of                                                 [APPLICATION SHEET C38]


Cryptosporidium (see “Protozoa”)


Cyclospora (see “Protozoa”)


Dendritic cells


Barrier flotation                                                                                       [APPLICATION SHEET C20]


Barrier sedimentation                                                                             [APPLICATION SHEET C46]


Mixer flotation                                                                                         [APPLICATION SHEET C21]


Enterocyozoon bieneusi (see “Protozoa”)


Erythrocytes (normal and sickle cells)                                               [APPLICATION SHEET C35]


Erythrocytes, removal from blood and bone marrow                       [APPLICATION SHEET C35]


Foam cells                                                                                             [APPLICATION SHEET C46]


Gastric cells


Parietal cells                                                                                           [APPLICATION SHEET C28]


ECL cells                                                                                                [APPLICATION SHEET C31]


Granulocytes (see “Polymorphonuclear leukocytes”)


Hepatic cells


Kupffer cells                                                                                           [APPLICATION SHEET C47]


Non-parenchymal cells                                                                          [APPLICATION SHEET C24]


Stellate cells                                                                                          [APPLICATION SHEET C33]


Kidney cells (see “Renal”)


Langerhans cells (see “Dendritic cells”)


Lymphocytes


Blood and tissues, from (see “Mononuclear cells”)


Macrophages                                                                                        [APPLICATION SHEET C45]


Mattesia orzaephili (see “Protozoa”)


Monocytes (human)


Leukocyte-rich plasma


   Barrier sedimentation                                                                          [APPLICATION SHEET C51]


   Flotation                                                                                               [APPLICATION SHEET C09]


Whole blood, from                                                                                  [APPLICATION SHEET C10]

 

Mononuclear cells


Bone marrow                                                                                          [APPLICATION SHEET C40]


Equine peripheral blood                                                                         [APPLICATION SHEET C08]


Human peripheral blood


   Barrier flotation                                                                                    [APPLICATION SHEET C05]


   Barrier sedimentation                                                                          [APPLICATION SHEET C03]


   Mixer flotation                                                                                      [APPLICATION SHEET C04]


Intestine                                                                                                  [APPLICATION SHEET C40]


Liver                                                                                                        [APPLICATION SHEET C40]

 

Mouse blood


   Barrier sedimentation                                                                          [APPLICATION SHEET C43]


   Mixer flotation                                                                                      [APPLICATION SHEET C07]


Non-human primate peripheral blood                                                     [APPLICATION SHEET C30]


Peritoneal exudates                                                                                [APPLICATION SHEET C19]


Rabbit blood                                                                                            [APPLICATION SHEET C43]


Rat blood


   Barrier sedimentation                                                                           [APPLICATION SHEET C43]


   Mixer flotation                                                                                       [APPLICATION SHEET C06]


Ruminant peripheral blood                                                                      [APPLICATION SHEET C08]


Spinal cord                                                                                              [APPLICATION SHEET C40]


Spleen                                                                                                     [APPLICATION SHEET C40]


Neural cells


Inflammatory cells (spinal cord injury)                                                    [APPLICATION SHEET C36]


Microglial cells                                                                                        [APPLICATION SHEET C36]


Motoneurons (brain, various sites)                                                         [APPLICATION SHEET C29]


Motoneurons (spinal cord)                                                                      [APPLICATION SHEET C22]


Oligodendrocytes (see “Microglial cells”)


Neutrophils

 

see “Polymorphonuclear leukocytes”


Pancreatic islets                                                                                   [APPLICATION SHEET C15]


Pancreatic stellate cells                                                                       [APPLICATION SHEET C33]


Plant protoplasts                                                                                  [APPLICATION SHEET C18]


Platelets (human)                                                                                 [APPLICATION SHEET C12]


Polymorphonuclear leukocytes


Human peripheral blood                                                                         [APPLICATION SHEET C11]


Mouse blood                                                                                           [APPLICATION SHEET C44]

 

Non-human primate peripheral blood (see “Human


peripheral blood”)


Peritoneal exudates                                                                                [APPLICATION SHEET C19]


Rat blood (see “ Mouse blood”)


Rabbit blood (see “ Mouse blood”)


Spinal injury, quantitative assessment in                                                [APPLICATION SHEET C36]


Progenitor cells (bone marrow and other tissues)


Barrier sedimentation                                                                              [APPLICATION SHEET C23]


Protozoa


Cryptosporidium                                                                                      [APPLICATION SHEET C27]


Cyclospora                                                                                              [APPLICATION SHEET C27]


Enterocytozoon bieneusi                                                                         [APPLICATION SHEET C27]


Mattesia orzaephili                                                                                  [APPLICATION SHEET C27]


Plasmodium                                                                                            [APPLICATION SHEET C41]


Sarcocystis neurona                                                                               [APPLICATION SHEET C27]


Toxoplasma


   Purification from cell cultures                                                               [APPLICATION SHEET C48]


   Separation of sporocysts and oocyst walls                                          [APPLICATION SHEET C34]


Pulmonary cells


Endothelial cells                                                                                      [APPLICATION SHEET C25]


Epithelial cells (Type I)                                                                            [APPLICATION SHEET C25]


Epithelial cells (Type II)                                                                           [APPLICATION SHEET C26]


Lymphoid cells                                                                                        [APPLICATION SHEET C26]


Macrophages                                                                                          [APPLICATION SHEET C25]


Myeloid cells                                                                                           [APPLICATION SHEET C26]


Renal cells


Interstitial and thin loop of Henlé cells                                                    [APPLICATION SHEET C42]


Proximal tubule                                                                                       [APPLICATION SHEET C42]


Reticulocytes                                                                                         [APPLICATION SHEET C35]


Sarcocystis neurona (see “Protozoa”)


Sea urchin coelomocytes                                                                     [APPLICATION SHEET C37]


Sickle cells (see “Erythrocytes”)


Sperm cells


Bovine                                                                                                     [APPLICATION SHEET C16]


Equine                                                                                                     [APPLICATION SHEET C16]


Elephant                                                                                                  [APPLICATION SHEET C16]


Gazelle                                                                                                    [APPLICATION SHEET C16]


Human                                                                                                     [APPLICATION SHEET C17]


Mouse                                                                                                      [APPLICATION SHEET C16]


Porcine                                                                                                     [APPLICATION SHEET C16]


Turkey/rooster                                                                                          [APPLICATION SHEET C16]


Spleen


Dendritic cells (see “Dendritic cells”)


Mononuclear cells (see “Mononuclear cells”)


Splenocytes (see “Mononuclear cells”)


Stem cells (see “Progenitor cells”)


Thymus


Dendritic cells (see “Dendritic cells”)


Thrombocytes (see “Platelets”)


Viable/non-viable cells                                                                          [APPLICATION SHEET C13]


                                                                                                                               


                                                                                                                                     September 2016