OPTIPREP APPLICATION SHEET INDEX
EUKARYOTIC AND PROKARYOTIC CELLS
♦ The Index is divided into two sections:
A. General methods for preparing and analysing gradients
B. An alphabetical cell type index. Commonly isolated cells such as mononuclear
cells are often further categorized into species or tissue source. If a cell type is not
in this index, it may be necessary to develop a customized method: in Section A
see “Fractionation of a mixed population of cells” for some guidance.
♦ Click on the relevant [APPLICATION SHEET] for a detailed protocol. In some cases
more than one Application Sheet for a specific cell type may be provided if different
practical strategies are available.
A. GENERAL METHODS
Preparation of gradient solutions [APPLICATION SHEET C01]
Preparation of gradients [APPLICATION SHEET C02]
Fractionation of a mixed population of cells [APPLICATION SHEET C14]
Analysis of gradients [APPLICATION SHEET C49]
B. ALPHABETICAL CELL INDEX
Alveolar cells (see “Pulmonary cells”)
Apoptosis analysis [APPLICATION SHEET C32]
Bacteria
Anaplasma phagocytophilum [APPLICATION SHEET C50]
Chlamydophila abortus [APPLICATION SHEET C50]
Cyanobacteria [APPLICATION SHEET C50]
Listeria monocytogenes [APPLICATION SHEET C50]
Obligate intracellular bacteria [APPLICATION SHEET C50]
Piscirickettsia [APPLICATION SHEET C50]
Rickettsia typhi [APPLICATION SHEET C50]
Spore protoplast density determination [APPLICATION SHEET C50]
Soil, clinical specimens and food, from [APPLICATION SHEET C39]
Cells in suspension, maintenance of [APPLICATION SHEET C38]
Cryptosporidium (see “Protozoa”)
Cyclospora (see “Protozoa”)
Dendritic cells
Barrier flotation [APPLICATION SHEET C20]
Barrier sedimentation [APPLICATION SHEET C46]
Mixer flotation [APPLICATION SHEET C21]
Enterocyozoon bieneusi (see “Protozoa”)
Erythrocytes (normal and sickle cells) [APPLICATION SHEET C35]
Erythrocytes, removal from blood and bone marrow [APPLICATION SHEET C35]
Foam cells [APPLICATION SHEET C45]
Gastric cells
Parietal cells [APPLICATION SHEET C28]
ECL cells [APPLICATION SHEET C31]
Granulocytes (see “Polymorphonuclear leukocytes”)
Hepatic cells
Kupffer cells [APPLICATION SHEET C47]
Non-parenchymal cells [APPLICATION SHEET C24]
Stellate cells [APPLICATION SHEET C33]
Kidney cells (see “Renal”)
Langerhans cells (see “Dendritic cells”)
Lymphocytes
Blood and tissues, from (see “Mononuclear cells”)
Macrophages [APPLICATION SHEET C45]
Mattesia orzaephili (see “Protozoa”)
Monocytes (human)
Leukocyte-rich plasma
Barrier sedimentation [APPLICATION SHEET C51]
Flotation [APPLICATION SHEET C09]
Whole blood, from [APPLICATION SHEET C10]
Mononuclear cells
Bone marrow [APPLICATION SHEET C40]
Equine peripheral blood [APPLICATION SHEET C08]
Human peripheral blood
Barrier flotation [APPLICATION SHEET C05]
Barrier sedimentation [APPLICATION SHEET C03]
Mixer flotation [APPLICATION SHEET C04]
Intestine [APPLICATION SHEET C40]
Liver [APPLICATION SHEET C40]
Mouse blood
Barrier sedimentation [APPLICATION SHEET C43]
Mixer flotation [APPLICATION SHEET C07]
Non-human primate peripheral blood [APPLICATION SHEET C30]
Peritoneal exudates [APPLICATION SHEET C19]
Rabbit blood [APPLICATION SHEET C43]
Rat blood
Barrier sedimentation [APPLICATION SHEET C43]
Mixer flotation [APPLICATION SHEET C06]
Ruminant peripheral blood [APPLICATION SHEET C08]
Spinal cord [APPLICATION SHEET C40]
Spleen [APPLICATION SHEET C40]
Neural cells
Inflammatory cells (spinal cord injury) [APPLICATION SHEET C36]
Microglial cells [APPLICATION SHEET C36]
Motoneurons (brain, various sites) [APPLICATION SHEET C29]
Motoneurons (spinal cord) [APPLICATION SHEET C22]
Oligodendrocytes (see “Microglial cells”)
Neutrophils
see “Polymorphonuclear leukocytes”
Pancreatic islets [APPLICATION SHEET C15]
Pancreatic stellate cells [APPLICATION SHEET C33]
Plant protoplasts [APPLICATION SHEET C18]
Platelets (human) [APPLICATION SHEET C12]
Polymorphonuclear leukocytes
Human peripheral blood [APPLICATION SHEET C11]
Mouse blood [APPLICATION SHEET C44]
Non-human primate peripheral blood (see “Human
peripheral blood”)
Peritoneal exudates [APPLICATION SHEET C19]
Rat blood (see “ Mouse blood”)
Rabbit blood (see “ Mouse blood”)
Spinal injury, quantitative assessment in [APPLICATION SHEET C36]
Progenitor cells (bone marrow and other tissues)
Barrier sedimentation [APPLICATION SHEET C23]
Protozoa
Cryptosporidium [APPLICATION SHEET C27]
Cyclospora [APPLICATION SHEET C27]
Enterocytozoon bieneusi [APPLICATION SHEET C27]
Mattesia orzaephili [APPLICATION SHEET C27]
Plasmodium [APPLICATION SHEET C41]
Sarcocystis neurona [APPLICATION SHEET C27]
Toxoplasma
Purification from cell cultures [APPLICATION SHEET C48]
Separation of sporocysts and oocyst walls [APPLICATION SHEET C34]
Pulmonary cells
Endothelial cells [APPLICATION SHEET C25]
Epithelial cells (Type I) [APPLICATION SHEET C25]
Epithelial cells (Type II) [APPLICATION SHEET C26]
Lymphoid cells [APPLICATION SHEET C26]
Macrophages [APPLICATION SHEET C25]
Myeloid cells [APPLICATION SHEET C26]
Renal cells
Interstitial and thin loop of Henlé cells [APPLICATION SHEET C42]
Proximal tubule [APPLICATION SHEET C42]
Reticulocytes [APPLICATION SHEET C35]
Sarcocystis neurona (see “Protozoa”)
Sea urchin coelomocytes [APPLICATION SHEET C37]
Sickle cells (see “Erythrocytes”)
Sperm cells
Bovine [APPLICATION SHEET C16]
Equine [APPLICATION SHEET C16]
Elephant [APPLICATION SHEET C16]
Gazelle [APPLICATION SHEET C16]
Human [APPLICATION SHEET C17]
Mouse [APPLICATION SHEET C16]
Porcine [APPLICATION SHEET C16]
Turkey/rooster [APPLICATION SHEET C16]
Spleen
Dendritic cells (see “Dendritic cells”)
Mononuclear cells (see “Mononuclear cells”)
Splenocytes (see “Mononuclear cells”)
Stem cells (see “Progenitor cells”)
Thymus
Dendritic cells (see “Dendritic cells”)
Thrombocytes (see “Platelets”)
Viable/non-viable cells [APPLICATION SHEET C13]
September 2016