iSeq 100 Sequencing System
makes next-generation sequencing easier and more affordable than ever. Designed for simplicity, it allows labs of all sizes to sequence DNA and RNA at the push of a button.
Specifications for theiSeq 100 System
Lightweight, compact instrument makes sequencing simple while maintaining high data quality.
Instrument Specifications
| Run Time | 9.5–19 hrs |
|---|---|
| Instrument Dimensions | 42.5 cm × 30.5 cm × 33 cm (16.8 in × 12 in × 13 in) |
| Weight | 15.9 kg (35 lb) |
Cluster Generation and Sequencing*
| Read Length | Total Time | Output |
|---|---|---|
| 1 × 36 bp | 9.5 hrs | 144 Mb |
| 1 × 50 bp | 10 hrs | 200 Mb |
| 1 × 75 bp | 11 hrs | 300 Mb |
| 2 × 75 bp | 14 hrs | 600 Mb |
| 2 × 150 bp | 19 hrs | 1.2 Gb |
*Install specifications are based on Illumina PhiX control library at supported cluster densities (174–200 k/mm2 clusters passing filter). Total times include cluster generation, sequencing, and base calling with quality scores on an iSeq 100 System.
Discover
Introduction
In the dynamic landscape of genomics, the iSeq 100 Sequencing System emerges as a transformative force, empowering researchers with a compact yet powerful platform for high-throughput sequencing. This cutting-edge system redefines the boundaries of genomic exploration, offering unparalleled accuracy, flexibility, and scalability for a diverse range of applications.
Key Features
1. Compact Powerhouse
The iSeq 100 Sequencing System proves that big things come in small packages. Its compact design belies its robust capabilities, making it an ideal solution for laboratories with space constraints without compromising on sequencing power.
2. High Throughput, Low Input
Designed for versatility, the iSeq 100 delivers high-throughput sequencing with minimal input requirements. This system maximizes efficiency, enabling researchers to extract meaningful insights from precious samples, even in scenarios with limited starting material.
3. Rapid Turnaround Time
Time is of the essence in genomics research, and the iSeq 100 rises to the challenge. With rapid turnaround times, researchers can accelerate their experiments, from targeted gene panels to whole-genome sequencing, without sacrificing data quality.
4. Seamless Workflow Integration
Integration into existing workflows is seamless with the iSeq 100. Its user-friendly interface, coupled with automated processes, minimizes hands-on time and reduces the potential for experimental variability. Researchers can focus on data analysis and interpretation rather than intricate technicalities.
Advantages
1. Cost-Effective Sequencing
The iSeq 100 is a cost-effective solution that democratizes sequencing capabilities. Researchers can achieve robust results without the prohibitive costs often associated with high-throughput sequencing systems, making genomics research accessible to a broader scientific community.
2. Customizable Applications
This sequencing system caters to a spectrum of applications, from targeted sequencing and microbial genomics to small genome sequencing. Its adaptability ensures that researchers can tailor their experiments to suit the unique requirements of their genomic inquiries.
3. User-Friendly Operation
A user-friendly interface and intuitive controls make the iSeq 100 accessible to researchers of all expertise levels. From experimental setup to data analysis, the system simplifies the sequencing process, allowing users to focus on the scientific questions at hand.
Applications
1. Cancer Genomics
Explore the intricacies of cancer genomes with the iSeq 100, uncovering mutations, structural variations, and insights crucial for personalized medicine and targeted therapies.
2. Microbial Genomics
Unravel the mysteries of microbial communities with high-throughput sequencing. The iSeq 100 is an invaluable tool for metagenomics, providing in-depth analysis of diverse microbial populations.
3. Clinical Diagnostics
In the realm of clinical diagnostics, the iSeq 100 offers a reliable platform for identifying genetic variants associated with inherited disorders, enabling precision medicine approaches in patient care.
Reads or Clusters Passing Filter
| Single Reads | 4 million |
| Paired-End Reads | 8 million |
Quality Scores*
| > 85% of bases higher than Q30 at 1 × 36 bp |
| > 85% of bases higher than Q30 at 1 × 50 bp |
| > 80% of bases higher than Q30 at 1 × 75 bp |
| > 80% of bases higher than Q30 at 2 × 75 bp |
| > 80% of bases higher than Q30 at 2 × 150 bp |
*The percentage of bases > Q30 is averaged across the entire run.
iSeq 100 System: Versatile Sequencing for a Variety of Applications
The iSeq 100 System is a versatile sequencing platform that can be used for a variety of applications, including:
- Small whole-genome sequencing of bacteria, viruses, and plasmids
- Targeted sequencing of a set of genes or gene regions
- Gene expression analysis
- 16S metagenomics
Small whole-genome sequencing
The iSeq 100 System can be used to sequence the entire genome of small organisms, such as bacteria, viruses, and plasmids. This information can be used to study the evolution of these organisms, identify new genes or proteins, and develop new diagnostic tests and treatments.
Targeted sequencing
The iSeq 100 System can also be used to sequence specific genes or regions of interest. This is useful for a variety of applications, such as identifying genetic mutations associated with diseases, studying gene expression in different cells or tissues, and developing new targeted therapies.
Gene expression analysis
The iSeq 100 System can be used to study how genes are expressed in different cells or tissues. This information can be used to understand how genes are regulated and how they contribute to different diseases and conditions.
16S metagenomics
The iSeq 100 System can also be used for 16S metagenomics, which is the study of the different microbes present in a sample. This information can be used to study the diversity of microbial communities in different environments, identify new microbes, and develop new diagnostic tests and treatments for infectious diseases.
The iSeq 100 System is a powerful and versatile sequencing platform that can be used for a wide range of applications. It is a valuable tool for researchers in a variety of fields, including biology, medicine, and environmental science.
| Feature | iSeq 100 | MiniSeq | MiSeq Series | NextSeq 550 Series | NextSeq 1000 & 2000 |
|---|---|---|---|---|---|
| Maximum read length (paired-end) | 150 bp | 150 bp | 250 bp | 300 bp | 300 bp |
| Maximum output per run (paired-end) | 140 Gb | 300 Gb | 600 Gb | 600 Gb | 1200 Gb |
| Typical run time (paired-end) | 26 hours | 20 hours | 26 hours | 18 hours | 16 hours |
| Supported applications | Small whole-genome sequencing, targeted gene sequencing, transcriptome sequencing, targeted gene expression profiling, miRNA & small RNA analysis, 16S metagenomic sequencing | Small whole-genome sequencing, targeted gene sequencing, transcriptome sequencing, targeted gene expression profiling, miRNA & small RNA analysis, 16S metagenomic sequencing, metagenomic profiling, cell-free sequencing & liquid biopsy analysis | Large whole-genome sequencing, exome & large panel sequencing, targeted gene sequencing, single-cell profiling, transcriptome sequencing, targeted gene expression profiling, miRNA & small RNA analysis, DNA-protein interaction analysis, methylation sequencing, 16S metagenomic sequencing, metagenomic profiling, cell-free sequencing & liquid biopsy analysis | Large whole-genome sequencing, exome & large panel sequencing, targeted gene sequencing, single-cell profiling, transcriptome sequencing, targeted gene expression profiling, miRNA & small RNA analysis, DNA-protein interaction analysis, methylation sequencing, 16S metagenomic sequencing, metagenomic profiling, cell-free sequencing & liquid biopsy analysis | Large whole-genome sequencing, exome & large panel sequencing, targeted gene sequencing, single-cell profiling, transcriptome sequencing, targeted gene expression profiling, miRNA & small RNA analysis, DNA-protein interaction analysis, methylation sequencing, 16S metagenomic sequencing, metagenomic profiling, cell-free sequencing & liquid biopsy analysis |
Prep
Prepare libraries for a range of targeted applications, including microbial sequencing and targeted resequencing.
Sequence
Start a run on the iSeq 100 System in less than 5 minutes. Sequencing is complete between 9.5 and 19 hours pending run size.
Analyze
Monitor and analyze sequencing runs using the on-instrument software and touch-screen interface.
Share
Interface with the iSeq 100 System from any web browser to review real-time data and performance metrics.
Conclusion
The iSeq 100 Sequencing System stands at the forefront of genomic innovation, offering a compact yet powerful solution for researchers hungry for high-throughput sequencing capabilities. With its impressive features, advantages, and diverse applications, the iSeq 100 is poised to revolutionize the genomics landscape. Elevate your research, unlock genomic mysteries—choose the iSeq 100 for a future where genomic discovery knows no bounds.