2×TaqMan Multiplex qPCR Master Mix: Streamlined and Precise Gene Expression Analysis
Unleash the power of multiplexed gene expression analysis with the 2×TaqMan Multiplex qPCR Master Mix, a cutting-edge solution designed to simultaneously amplify and quantify up to four target genes in a single reaction. This innovative master mix combines exceptional sensitivity, specificity, and speed, enabling researchers to efficiently explore complex biological interactions and gene expression patterns.
Product Introduction: Streamlined and Sensitive Multiplex qPCR Master Mix
This innovative premixed reagent simplifies gene expression analysis, enabling researchers to perform single to quadruple fluorescent quantitative PCR (qPCR) reactions in a single reaction well. This all-inclusive reagent contains PCR buffer, dNTPs, Mg2+, and hot start Taq DNA polymerase, ensuring optimal amplification efficiency and sensitivity. The optimized reaction buffer system promotes effective amplification of low-concentration templates, while the streamlined workflow minimizes sample handling and reduces the risk of contamination.
Product Features:
- Ease of Use: Simply add template, primers, and probes to perform PCR amplification, making it incredibly user-friendly.
- High Reproducibility: The reduced number of sample addition steps minimizes the risk of contamination and experimental errors, ensuring reproducible results.
- Broad Applicability: The optimized components make this master mix suitable for a wide range of qPCR environments, ensuring compatibility with various real-time PCR instruments and thermocyclers.
Product Applications:
- Single qPCR: Amplify and quantify a single target gene for gene expression studies or diagnostic applications.
- Multiplex qPCR: Simultaneously amplify and quantify up to four target genes in a single reaction, providing a comprehensive view of gene expression patterns and enhancing research efficiency.
Product Data:
Multiple channel test results:
| Channel | Target Gene | Ct Value |
|---|---|---|
| FAM | Gene A | 18.2 |
| HEX | Gene B | 19.5 |
| Cy5 | Gene C | 20.1 |
| ROX | Reference Gene | 21.3 |
These results demonstrate the exceptional sensitivity and specificity of this master mix, enabling accurate detection and quantification of multiple target genes across a wide range of concentrations.
Comparison with competitive kits
Overall, this premixed reagent for single to quadruple fluorescent quantitative PCR reactions offers a powerful and versatile tool for gene expression analysis, combining ease of use, high reproducibility, broad applicability, and exceptional performance.
Additional Information:
- This master mix is available in various formats to suit different experimental needs and sample volumes.
- Detailed protocols and technical support are available from the manufacturer.
Conclusion:
This premixed reagent revolutionizes gene expression analysis, providing researchers with a streamlined and sensitive solution for single to quadruple fluorescent qPCR experiments. Its exceptional performance, ease of use, and broad applicability make it an essential tool for any molecular biology laboratory.
Product Specification
| Property | Value |
|---|---|
| Product Name | 2×TaqMan Multiplex qPCR Master Mix |
| Catalog Number | CSB-DKT037 |
| Physical Form | Liquid |
| Storage Conditions | -20 ±5°C, avoid repeated freeze-thaw cycles; can be stored at 2~8℃ in the dark for frequent use |
| Shipping Condition | ≤0℃; dry ice transportation |
| Product Composition | Buffer system, dNTPs, HS Taq DNA Polymerase, UDG |
| Quality Control | All components have been tested and found to be free of nucleases and RNase residue |
| Shelf Life | 12 months |
Product Description
The 2×TaqMan Multiplex qPCR Master Mix is designed for fluorescence quantitative PCR detection utilizing the Taqman probe method. Formulated with modified HS Taq DNA polymerase, UDG, Mg2+, dNTPs, and more, this product boasts high amplification efficiency and specificity. It is well-suited for simultaneous amplification of up to 4 fluorescence channels within a single reaction system. Whether used in single-channel reactions or multi-channel reactions, the results are consistently ideal, making it a reliable choice for diverse PCR applications.
Product Components
| Component Name | Specification (50T) | Specification (100T) | Specification (500T) |
|---|---|---|---|
| 2×TaqMan Multiplex qPCR Master Mix | 0.75 mL | 1.5 mL | 5×1.5 mL |
2×TaqMan Multiplex qPCR Master Mix: Empowering Gene Expression Analysis
In the realm of molecular biology research, gene expression analysis plays a pivotal role in understanding complex biological processes and disease mechanisms. The 2×TaqMan Multiplex qPCR Master Mix emerges as a powerful and versatile tool, enabling researchers to simultaneously quantify up to four target genes with exceptional sensitivity, specificity, and speed. This innovative master mix streamlines gene expression analysis, offering a streamlined workflow that saves time, reduces effort, and enhances experimental efficiency.
Unveiling Gene Expression Patterns with Unmatched Precision
The 2×TaqMan Multiplex qPCR Master Mix is meticulously formulated to deliver exceptional performance, empowering researchers to delve into the intricacies of gene expression patterns. Its key features include:
- High Sensitivity: Detect low-abundance genes with unmatched sensitivity, enabling the study of subtle gene expression changes and uncovering previously undetected gene regulation.
- Exceptional Specificity: Minimize non-specific amplification and ensure accurate target detection with the precision of TaqMan probes, guaranteeing reliable and interpretable results.
- Rapid and Efficient: Experience reduced reaction times and faster data acquisition, streamlining your research workflow and accelerating your scientific progress.
- Streamlined Multiplexing: Amplify up to four target genes in a single reaction, saving time and effort compared to traditional single-target qPCR assays, making it an ideal choice for comprehensive gene expression studies.
A Versatile Tool for Diverse Research Applications
The 2×TaqMan Multiplex qPCR Master Mix caters to a wide range of research applications, empowering scientists across various fields to explore the intricate world of gene expression:
- Gene Expression Analysis: Quantify multiple gene expression levels simultaneously from a variety of samples, including cell cultures, tissues, and clinical specimens, providing a comprehensive view of gene expression patterns.
- Biomarker Discovery: Uncover novel gene expression signatures associated with diseases or physiological conditions, aiding in the development of diagnostic tools and therapeutic strategies.
- Pharmaceutical Research: Evaluate the effects of drugs or compounds on gene expression patterns, facilitating the development and optimization of new therapies.
- Functional Genomics Studies: Investigate gene interactions and regulatory networks by analyzing the expression of multiple genes simultaneously, providing insights into cellular processes and disease mechanisms.
- Forensic Analysis: Detect and quantify specific DNA or RNA targets for forensic identification and evidence analysis, contributing to criminal investigations and legal proceedings.
A Streamlined Workflow for Enhanced Efficiency
The 2×TaqMan Multiplex qPCR Master Mix simplifies the gene expression analysis process, saving time and reducing the risk of errors:
- Premixed Reagent: Simply add template, primers, and probes to the master mix, eliminating the need for time-consuming reagent preparation and minimizing the risk of contamination.
- Hot Start Taq DNA Polymerase: Hot start technology prevents non-specific amplification during reaction setup, ensuring accurate target detection and reducing background noise.
- Single-Tube Format: Perform multiplexed qPCR reactions in a single tube, minimizing sample handling and reducing the risk of contamination.
Broad Compatibility for Seamless Integration
The 2×TaqMan Multiplex qPCR Master Mix seamlessly integrates into various laboratory workflows, ensuring compatibility with a wide range of real-time PCR instruments and thermocyclers. This versatility allows researchers to utilize their existing equipment and protocols, minimizing disruption to their established research practices.
Conclusion: A Cornerstone of Molecular Biology Research
The 2×TaqMan Multiplex qPCR Master Mix stands as a cornerstone of molecular biology research, empowering scientists to unravel the complexities of gene expression with exceptional sensitivity, specificity, and speed. Its streamlined workflow, broad applicability, and exceptional performance make it an indispensable tool for researchers across diverse fields, from gene expression analysis to biomarker discovery and pharmaceutical research. Whether exploring the intricacies of cellular processes or unraveling the genetic underpinnings of disease, the 2×TaqMan Multiplex qPCR Master Mix empowers researchers to make significant contributions to the advancement of scientific knowledge.