Axis-Shield Density Gradient Media

  -a brand of Alere Technologies AS, Oslo, Norway

OPTIPREP APPLICATION SHEET INDEX

MACROMOLECULES & MACROMOLECULAR COMPLEXES


The Index is divided into two sections:


A. General methods for preparing and analyzing gradients

B. An alphabetical macromolecule/macromolecular complex type index.

 

Click the relevant (Application sheet) for a detailed protocol. More than one Application sheet may be available for a specific macromolecule/macromolecular complex type if different practical strategies are available.


A. GENERAL METHODS


Preparation of gradient solutions                                                   [APPLICATION SHEET M01]


Preparation of discontinuous and continuous gradients                [APPLICATION SHEET M02]


Preparation of self-generated gradients                                        [APPLICATION SHEET M03]


Harvesting gradients                                                                      [APPLICATION SHEET M04]


Analysis of gradients                                                                      [APPLICATION SHEET M05]


B. ALPHABETICAL MACROMOLECULE/COMPLEX INDEX


Cytoskeleton                                                                                [APPLICATION SHEET M09]


Liposome-encapsulated macromoleules, purification             [APPLICATION SHEET M10]


Microtubules                                                                                 [APPLICATION SHEET M09]


Myosin II                                                                                        [APPLICATION SHEET M09]


Nucleic acids                                                                                [APPLICATION SHEET M13]


Nucleic acid-protein complexes                                                 [APPLICATION SHEET M13]


Plasma lipoproteins


     HDL, LDL and VLDL analysis                                                   [APPLICATION SHEET M07]


     Lipoprotein sub-class analysis                                                  [APPLICATION SHEET M08]


Plasmid DNA purification                                                            [APPLICATION SHEET M06]


Polysaccharides (buoyant density banding)                             [APPLICATION SHEET M12]


Prion proteins                                                                               [APPLICATION SHEET M09]


Proteins

 

     Complex formation analysis                                                      [APPLICATION SHEET M09]


     Mitochondrial proteins (see “Nucleic acid-protein complexes”)


     Size analysis in pre-formed gradients                                       [APPLICATION SHEET M11]


     Size analysis in self-generated gradients                                  [APPLICATION SHEET M14]


Proteoliposomes (separation from proteins)                             [APPLICATION SHEET M10]