AXIS-SHIELD Density Gradient Media

  -a brand of Alere Technologies AS, Oslo, Norway

OPTIPREP APPLICATION SHEET INDEX

MACROMOLECULES & MACROMOLECULAR COMPLEXES


The Index is divided into two sections:


A. General methods for preparing and analyzing gradients

B. An alphabetical macromolecule/macromolecular complex type index.

 

Click the relevant (Application sheet) for a detailed protocol. More than one Application sheet may be available for a specific macromolecule/macromolecular complex type if different practical strategies are available.


A. GENERAL METHODS


Preparation of gradient solutions                                                   [APPLICATION SHEET M01]


Preparation of discontinuous and continuous gradients                [APPLICATION SHEET M02]


Preparation of self-generated gradients                                        [APPLICATION SHEET M03]


Harvesting gradients                                                                      [APPLICATION SHEET M04]


Analysis of gradients                                                                      [APPLICATION SHEET M05]


B. ALPHABETICAL MACROMOLECULE/COMPLEX INDEX


Cytoskeleton                                                                                [APPLICATION SHEET M09]


Liposome-encapsulated macromoleules, purification             [APPLICATION SHEET M10]


Microtubules                                                                                 [APPLICATION SHEET M09]


Myosin II                                                                                        [APPLICATION SHEET M09]


Nucleic acids                                                                                [APPLICATION SHEET M13]


Nucleic acid-protein complexes                                                 [APPLICATION SHEET M13]


Plasma lipoproteins


     HDL, LDL and VLDL analysis                                                   [APPLICATION SHEET M07]


     Lipoprotein sub-class analysis                                                  [APPLICATION SHEET M08]


Plasmid DNA purification                                                            [APPLICATION SHEET M06]


Polysaccharides (buoyant density banding)                             [APPLICATION SHEET M12]


Prion proteins                                                                               [APPLICATION SHEET M09]


Proteins

 

     Complex formation analysis                                                      [APPLICATION SHEET M09]


     Mitochondrial proteins (see “Nucleic acid-protein complexes”)


     Size analysis in pre-formed gradients                                       [APPLICATION SHEET M11]


     Size analysis in self-generated gradients                                  [APPLICATION SHEET M14]


Proteoliposomes (separation from proteins)                             [APPLICATION SHEET M10]



 

Latest news

 Axis-Shield will exhibit at the ASCB meeting in San Francisco December 3-7. You will find us in booth 418

 

We have recently posted a new package insert for Polymorphprep (see Products in the main menu). In addition to a new figure, we have added a new paragraph; Important practical and sample notes.

 

We have decided to discontinue the product NycoPrep 1.077, 4x250 ml. We still have lot no. 12GLS12 in stock, with an extended expiry date of November 2017.