Axis-Shield Density Gradient Media

  -a brand of Alere Technologies AS, Oslo, Norway

OPTIPREP APPLICATION SHEET INDEX VIRUSES


Since the first papers reporting the use of density gradients, prepared from OptiPrep were

published in 1998-1999, Application Sheets providing detailed protocols and reviews of

methodologies have been produced and updated, on an ad hoc basis, as new information became

available in scientific journals. Today OptiPrep has been used for the purification and analysis

of over 80 types of virus; we have therefore now reorganized all of these Application Sheets.

 

All of the general information on gradient production and analysis plus pre-gradient virus

concentration are contained in Application Sheets V01-V06.

 

All the virus gradient protocols have been rearranged into the seven groups of the Baltimore

Virus Classification scheme (V07-V36).

 

Within each group, Families (sometimes preceded by the Order name), and where appropriate

Subfamilies, are listed alphabetically in italics. Within each Family or Subfamily individual

viruses are also listed alphabetically.

 

Click on the relevant [APPLICATION SHEET] for a detailed protocol of virus isolation.


In some cases more than one Application Sheet for a specific virus type may be provided, if

significantly different practical strategies are available.


Protocols for one particular virus in a Group may be applicable to other viruses in the same group

with a similar overall structure and composition, but this can only be determined experimentally.


Yeast retrotransposons, although not part of Baltimore Virus Classification system, are

considered similar in structure to retroviruses. They are included in Group VI listings.


Plant Virus and Bacteriophage Application Sheets can accessed from this index after the entry

for Group VII viruses.


1. Gradient preparation and analysis and virus concentration


Preparation of density gradient solutions                                                [APPLICATION SHEET V01]


Preparation of discontinuous and continuous gradients                         [APPLICATION SHEET V02]


Preparation of self-generated gradients                                                  [APPLICATION SHEET V03]


Harvesting gradients                                                                               [APPLICATION SHEET V04]


Analysis of gradients                                                                               [APPLICATION SHEET V05]


Concentration of virus samples                                                               [APPLICATION SHEET V06]


2. Virus purification


Group I (ds)DNA viruses


Adenoviridae


     Adenovirus                                                                                          [APPLICATION SHEET V07]


     Helper-dependent virus, separation from                                            [APPLICATION SHEET V07]


Asfaviridae


     African swine fever virus                                                                     [APPLICATION SHEET V09]


Baculoviridae


     Baculovirus vectors                                                                             [APPLICATION SHEET V08]


Herpesviridae


     Cytomegalovirus


        Self-generated gradient                                                                    [APPLICATION SHEET V08]


        Pre-formed gradient                                                                          [APPLICATION SHEET V09]

 

     Epstein-Barr virus


        Self-generated gradient                                                                    [APPLICATION SHEET V08]


        Pre-formed gradient                                                                          [APPLICATION SHEET V09]


     Herpes simplex virus


        Self-generated gradient                                                                    [APPLICATION SHEET V08]


        Pre-formed gradient                                                                          [APPLICATION SHEET V09]


     Herpes virus vectors                                                                            [APPLICATION SHEET V08]


     Rhadinovirus                                                                                        [APPLICATION SHEET V09]


Iridoviridae


     Iridovirus                                                                                              [APPLICATION SHEET V13]


Papillomaviridae


     Human papillomavirus                                                                         [APPLICATION SHEET V10]


Phycodnaviridae


     Chlorovirus                                                                                          [APPLICATION SHEET V13]


Polyomaviridae


     BK virus                                                                                               [APPLICATION SHEET V11]


     JC virus                                                                                                [APPLICATION SHEET V11]


     Merkel cell carcinoma virus                                                                 [APPLICATION SHEET V11]


     Simian virus 40 (SV40)                                                                        [APPLICATION SHEET V11]


Poxviridae


     Molluscipoxvirus                                                                                   [APPLICATION SHEET V12]


     Vaccinia virus                                                                                       [APPLICATION SHEET V12]


Group II (ss)DNA viruses


Anelloviridae


     Torque teno virus                                                                                 [APPLICATION SHEET V15]


Parvoviridae


Densovirinae


Dependovirus


     Avian adeno-associated virus                                                              [APPLICATION SHEET V14]


     Recombinant adeno-associated virus                                                  [APPLICATION SHEET V14]


Parvovirinae


     Minute virus of mice                                                                             [APPLICATION SHEET V15]


     Parvovirus                                                                                            [APPLICATION SHEET V16]


Group III (ds)RNA viruses


Reoviridae


Birnavirus


     Espirito Santo virus                                                                              [APPLICATION SHEET V17]


Sedoreovirinae


     Rotavirus                                                                                              [APPLICATION SHEET V17]


     Seadornavirus                                                                                      [APPLICATION SHEET V17]


Spinareovirinae


     Dinovernavirus                                                                                     [APPLICATION SHEET V17]


Group IV ((+)ss)RNA viruses


Caliciviridae


Lagovirus


     Rabbit haemorrhagic fever virus                                                          [APPLICATION SHEET V18]


Norovirus


     Norwalk virus                                                                                        [APPLICATION SHEET V18]


Group IV ((+)ss) RNA viruses (contd.)


Flaviviridae


Flavivirus


     Dengue virus                                                                                        [APPLICATION SHEET V19]


     West Nile virus                                                                                     [APPLICATION SHEET V19]


     Yellow fever virus                                                                                 [APPLICATION SHEET V19]


Hepacivirus


     Hepatitis C virus


        Pre-formed gradient                                                                          [APPLICATION SHEET V19]


        Self-generated                                                                                  [APPLICATION SHEET V20]


Hepevirus


     Hepatitis E virus                                                                                   [APPLICATION SHEET V19]


Pestivirus


     Bovine diarrhea virus                                                                           [APPLICATION SHEET V19]


Nidovirales - Arteriviridae


     Porcine reproductive & respiratory syndrome virus                             [APPLICATION SHEET V21]


Nidovirales - Coronaviridae


     SARS coronavirus                                                                               [APPLICATION SHEET V21]


Togaviridae


Alphavirus


     Chikungunya virus                                                                               [APPLICATION SHEET V22]


     Semliki Forest virus                                                                             [APPLICATION SHEET V22]


     Sindbis virus                                                                                        [APPLICATION SHEET V22]


     Venezuelan equine encephalitis virus                                                 [APPLICATION SHEET V22]


Rubivirus


     Rubella virus                                                                                        [APPLICATION SHEET V22]


Tombusviridae


Dianthovirus


     Red clover mosaic virus                                                                       [APPLICATION SHEET V22]


Group V ((-)ss) RNA viruses


Arenaviridae


Arenavirus


     Lassa virus                                                                                           [APPLICATION SHEET V23]


     Tacaribe virus                                                                                       [APPLICATION SHEET V23]


Bunyaviridae


Hantavirus


     Hantavirus                                                                                            [APPLICATION SHEET V24]


Orthobunyavirus


     Bunyamweravirus                                                                                 [APPLICATION SHEET V24]


Phlebovirus


     Rift valley fever virus                                                                            [APPLICATION SHEET V24]


Filoviridae


    Ebola virus                                                                                            [APPLICATION SHEET V25]


Orthomyxoviridae


     Influenza virus                                                                                      [APPLICATION SHEET V26]


Rhabdoviridae


Lyssavirus


     Rabies virus                                                                                          [APPLICATION SHEET V27]


Vesiculovirus


     Vesicular stomatitis virus                                                                      [APPLICATION SHEET V27]


Paramyxoviridae


Paramyxovirinae


Avulavirus


     Newcastle disease virus                                                                       [APPLICATION SHEET V28]


Morbillivirus


     Measles virus                                                                                        [APPLICATION SHEET V28]


Respirovirus


     Swine paramyxovirus                                                                            [APPLICATION SHEET V28]


Pneumovirinae


     Human respiratory syncytial virus                                                         [APPLICATION SHEET V28]


Group VI (ss)RNA-RT viruses


Retroviridae


Alpharetrovirus


     Rous sarcoma virus                                                                              [APPLICATION SHEET V29]


Betaretrovirus


     Mason-Pfizer monkey virus (Simian retrovirus)                                    [APPLICATION SHEET V30]


Deltaretrovirus


     Human T cell lymphotropic virus                                                          [APPLICATION SHEET V31]


Gammaretrovirus


     Moloney murine leukaemia virus                                                          [APPLICATION SHEET V33]


     Murine oncornavirus                                                                             [APPLICATION SHEET V32]


Lentivirus


     Feline immunodeficiency virus                                                             [APPLICATION SHEET V34]


     Human immunodeficiency virus                                                           [APPLICATION SHEET V34]


Spumaretroviridae


Spumavirus


     Foamy virus                                                                                          [APPLICATION SHEET V35]


Yeast retrotransposons                                                                             [APPLICATION SHEET V37]


Group VII (ds)RNA-RT


Hepadnaviridae


Orthohepadnavirus


     Hepatitis B                                                                                            [APPLICATION SHEET V36]


Plant viruses                                                                                            [APPLICATION SHEET V37]


Bacteriophages                                                                                       [APPLICATION SHEET V38]