EzWestBlue W: A Sensitive and Safe Substrate for HRP Detection
EzWestBlue W is a TMB substrate for horseradish peroxidase (HRP) used for the colorimetric detection of immunoblotting. It is a highly sensitive and safe alternative to DAB, producing a bright and clear blue signal. EzWestBlue W is also compatible with total protein normalization, making it a versatile tool for a variety of research applications.
WSE-7140 EzWestBlue W
EzWestBlue W is a colorimetric substrate specifically designed for the detection of horseradish peroxidase (HRP) in immunoblotting applications. It provides a highly sensitive and safe alternative to conventional substrates like DAB, producing a bright and clear blue signal that is easy to distinguish from background noise.
Applications of EzWestBlue W in Immunoblotting
Immunoblotting, also known as Western blotting, is a widely used technique in molecular biology, biochemistry, and immunology to identify and quantify specific proteins from complex samples. EzWestBlue W serves as a valuable substrate in this technique, offering several advantages over traditional substrates like DAB:
1. High Sensitivity Detection:
EzWestBlue W exhibits exceptional sensitivity, comparable to chemiluminescence detection methods. This enhanced sensitivity enables the detection of low-abundance proteins that may be difficult to visualize using other substrates. This capability is particularly useful in various research applications, such as studying protein expression levels under different conditions or detecting proteins with weak antibody binding affinities.
2. Safe Alternative to DAB:
Diaminobenzidine (DAB) has been a commonly used substrate for immunoblotting, but it poses potential health hazards due to its carcinogenic properties. EzWestBlue W, on the other hand, is considered a safe and non-toxic substrate, eliminating the safety concerns associated with DAB. This makes EzWestBlue W a preferred choice for researchers seeking a safer alternative without compromising sensitivity.
3. Clear Blue Signal Visualization:
The blue signal produced by EzWestBlue W is bright and easily distinguishable from the background, providing a clear contrast that facilitates protein detection and analysis. This clarity is particularly advantageous when working with samples containing complex backgrounds or when multiple proteins are being visualized simultaneously.
4. Compatibility with Total Protein Normalization:
Total protein normalization is a technique used to correct for variations in protein loading between samples, ensuring accurate protein quantification. EzWestBlue W is compatible with total protein normalization procedures, allowing researchers to obtain reliable and reproducible results.
5. Absence of Enzyme Inhibition:
The absence of organic solvents in EzWestBlue W eliminates the potential for enzyme inhibition, ensuring optimal horseradish peroxidase (HRP) activity and signal generation. This ensures that the sensitivity of the substrate is not compromised by the presence of inhibitors, leading to accurate protein detection.
In summary, EzWestBlue W offers a combination of high sensitivity, safety, clear signal visualization, compatibility with total protein normalization, and the absence of enzyme inhibition, making it a valuable tool for immunoblotting applications across various scientific disciplines.
| Specifications | WSE-7140 EzWestBlue W |
|---|---|
| Major Components | TMB (TMB 3,3',5,5' tetramethylbenzidine) solution (not containing organic solvents) |
| Package | 200 mL/bottle |
| Preparation | Ready to use |
| Applicable Amount | 0.1 mL/cm² |
| Serve | 2,000 cm² (approximately 25 sheets of mini-size gel) |
| Storage | 1 year when stored in a refrigerator |
| Ordering Information | |
| Code No. | Description |
| 2332456 | WSE-7140 EzWestBlue W |
Applications:
EzWestBlue W is primarily used for immunoblotting, a technique utilized in various fields of research, including molecular biology, biochemistry, and immunology, to identify and quantify specific proteins from complex samples.
Key Features:
- High Sensitivity: EzWestBlue W offers exceptional sensitivity, comparable to that of chemiluminescence detection methods. This allows for the detection of low-abundance proteins that may be difficult to visualize using other substrates.
- Safe Alternative: Unlike DAB, which can pose potential health hazards, EzWestBlue W is considered a safe and non-toxic substrate, making it a preferred choice for many researchers.
- Clear Blue Signal: The resulting blue signal produced by EzWestBlue W is bright and easily distinguishable, providing a clear contrast against the background.
- Total Protein Normalization: EzWestBlue W is compatible with total protein normalization techniques, allowing for the correction of variations in protein loading between samples.
- Absence of Enzyme Inhibition: The absence of organic solvents in EzWestBlue W eliminates the potential for enzyme inhibition, ensuring optimal HRP activity and signal generation.
Overall, EzWestBlue W stands out as a versatile and reliable substrate for immunoblotting, offering high sensitivity, safety, and clear signal visualization, making it a valuable tool for researchers across various scientific disciplines.