CpG Methyltransferase (M.SssI): A Versatile Tool for Epigenetics Research

 

CpG Methyltransferase (M.SssI) is a powerful enzyme that can be used to study DNA methylation, an important epigenetic modification that plays a role in gene expression and disease. This enzyme is derived from the bacterium Spiroplasma sp. strain MQ1 and has been used to study DNA methylation in a variety of organisms, including humans. M.SssI is a useful tool for epigenetics research because it is highly specific for the CpG dinucleotide, which is a common target for methylation in eukaryotic DNA.

 

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What is CpG Methyltransferase(M.SssI)?


CpG Methyltransferase (M.SssI) is an enzyme that catalyzes the transfer of a methyl group from S-adenosylmethionine (SAM) to the C5 position of cytosine residues in DNA. This enzyme is specifically formulated for fast reaction rates and high efficiency. It is used in a variety of molecular biology applications, including:

  • DNA methylation studies
  • Epigenetics research
  • Gene expression analysis
  • Disease research
How does CpG Methyltransferase(M.SssI) work?

CpG Methyltransferase (M.SssI) recognizes and methylates the C5 position of cytosine residues in CpG dinucleotides. CpG dinucleotides are found in all organisms, but they are particularly common in eukaryotic DNA. Methylation of CpG dinucleotides can have a profound effect on gene expression. For example, methylation of a promoter CpG dinucleotide can lead to silencing of the downstream gene.


What are the benefits of using CpG Methyltransferase(M.SssI)?



CpG Methyltransferase (M.SssI) is a powerful tool for epigenetics research because it is:

  • Highly specific for CpG dinucleotides
  • Fast and efficient
  • Stable and active under a variety of conditions
How to use CpG Methyltransferase(M.SssI)

CpG Methyltransferase (M.SssI) is typically used in a two-step reaction. The first step is to incubate the enzyme with SAM and the DNA substrate. The second step is to heat the reaction mixture to inactivate the enzyme.

Applications of CpG Methyltransferase(M.SssI)

CpG Methyltransferase (M.SssI) has a wide range of applications in molecular biology research. Some of the most common applications include:

  • DNA methylation studies
  • Epigenetics research
  • Gene expression analysis
  • Disease research
Safety considerations

CpG Methyltransferase (M.SssI) is an enzyme and should be handled with care. The enzyme is not harmful to humans, but it can be irritating to skin and eyes.

Where to buy CpG Methyltransferase(M.SssI)

CpG Methyltransferase (M.SssI) is available in Gentaur.

Product Introduction


CpG Methyltransferase (M.SssI) is a highly versatile and powerful enzyme that plays a crucial role in epigenetics research. Derived from recombinant E. coli strains harboring the CpG methyltransferase gene from Spiroplasma sp. MQ1 strain, this enzyme exhibits exceptional specificity for the CpG dinucleotide, a common target for methylation in eukaryotic DNA. Its unique properties and diverse applications make it an invaluable tool for exploring the intricate mechanisms of DNA methylation and its profound impact on gene expression.

Features


  • High Specificity: CpG Methyltransferase (M.SssI) demonstrates remarkable specificity towards the CpG dinucleotide, ensuring precise and selective methylation of target sites.
  • Enhanced Efficiency: The enzyme exhibits rapid reaction rates and high efficiency, enabling efficient methylation of DNA substrates.
  • Exceptional Stability: CpG Methyltransferase (M.SssI) maintains its activity and stability under a range of reaction conditions, ensuring consistent and reliable results.
  • Broad Applications: The enzyme finds applications in a wide spectrum of molecular biology research areas, including DNA methylation studies, epigenetics research, gene expression analysis, and disease research.
Benefits


  • Prevents Restriction Endonuclease Cleavage: Methylation of CpG dinucleotides by CpG Methyltransferase (M.SssI) effectively protects DNA from cleavage by restriction endonucleases, allowing for selective cloning and manipulation of DNA fragments.
  • Unveils CpG Methylation-Dependent Gene Expression: The enzyme enables the study of CpG methylation-mediated gene expression regulation, providing insights into the mechanisms of gene silencing and activation.
  • Alters DNA Physical Properties: Methylation of CpG dinucleotides by CpG Methyltransferase (M.SssI) alters the physical properties of DNA, influencing its interaction with DNA-binding proteins and chromatin structure.
  • Uniform Labeling with [3H]: The enzyme facilitates uniform labeling of DNA with [3H], enabling sensitive detection and quantification of methylated DNA sequences.
Applications


  • Methylation Modification: CpG Methyltransferase (M.SssI) is employed to modify DNA methylation patterns, enabling the study of the functional consequences of methylation changes.
  • Epigenetics Research: The enzyme serves as a valuable tool in epigenetics research, facilitating the investigation of DNA methylation dynamics and their role in various biological processes.
  • Gene Expression Analysis: CpG Methyltransferase (M.SssI) plays a crucial role in gene expression analysis, enabling the elucidation of the relationship between DNA methylation and gene regulation.
  • Disease Research: The enzyme finds applications in disease research, allowing for the study of DNA methylation alterations associated with various pathological conditions.

Overall, CpG Methyltransferase (M.SssI) stands as an indispensable tool for epigenetics research and molecular biology applications. Its high specificity, efficiency, and stability, coupled with its wide range of applications, make it a valuable asset for scientists exploring the intricate world of DNA methylation and its profound impact on gene expression and biological processes.