One Step RT-qPCR Kit: Revolutionizing Gene Expression Analysis
The One Step RT-qPCR Kit is an innovative and user-friendly solution for gene expression analysis, combining reverse transcription (RT) and real-time quantitative PCR (qPCR) into a single, contamination-free workflow. With its exceptional sensitivity, rapid performance, and ease of use, the One Step RT-qPCR Kit streamlines gene expression analysis, making it an essential tool for any molecular biology laboratory.
Streamlined and Sensitive One Step RT-qPCR Kit
The One Step RT-qPCR Kit revolutionizes gene expression analysis by seamlessly integrating reverse transcription (RT) and real-time quantitative PCR (qPCR) into a single, time-saving, and contamination-free workflow. This versatile kit utilizes RNA as a template and gene-specific primers to complete both RT and qPCR reactions within a single tube, eliminating the need for multiple opening and pipetting steps. By combining the superior performance of reverse transcriptase and hot start Taq DNA polymerase with an optimized buffer system, the One Step RT-qPCR Kit achieves exceptional sensitivity, enabling detection of as little as 0.5 pg of total RNA template. This streamlined approach significantly enhances detection throughput and reduces the risk of sample contamination, making it an ideal tool for a wide range of molecular biology applications.
Product Features:
- Exceptional Amplification Efficiency and Detection Sensitivity: The One Step RT-qPCR Kit outperforms similar products, delivering lower Ct values and enabling detection of lower starting RNA amounts.
- Ease of Use: The kit's simplified protocol requires only template and primers, eliminating the need for complex reaction setups.
- Rapid and Efficient: The One Step RT-qPCR Kit streamlines the PCR process, reducing sample addition steps and saving valuable time.
- Repeatable and Reliable: The single-tube format minimizes the risk of contamination and sample addition errors, ensuring consistent and reliable results.
Product Applications:
- Rapid Gene Cloning: The One Step RT-qPCR Kit facilitates efficient gene cloning from RNA templates.
- Colony PCR: The kit enables direct PCR amplification from bacterial colonies, eliminating the need for plasmid isolation.
- Genotyping: The kit simplifies genotyping applications by combining RNA-based target identification with direct PCR amplification.
- TA Cloning: The kit streamlines TA cloning procedures by integrating RT and PCR into a single step.
Product Data:
Comparison with similar products:
| Feature | One Step RT-qPCR Kit | Competitive Product 1 | Competitive Product 2 |
|---|---|---|---|
| Ct Value (Lower RNA Amount) | Smaller | Larger | Larger |
| Sensitivity | 0.5 pg total RNA | 1 pg total RNA | 2 pg total RNA |
| Ease of Use | Simpler protocol | More complex protocol | Complex protocol |
| Reaction Time | Faster | Slower | Slower |
| Reproducibility | Higher | Lower | Lower |
The One Step RT-qPCR Kit emerges as a superior choice for researchers seeking a streamlined, sensitive, and reliable tool for gene expression analysis and other molecular biology applications. Its ease of use, rapid performance, and exceptional reproducibility make it an invaluable asset for any laboratory.
Product Specification
| Property | Value |
|---|---|
| Product Name | One Step RT-qPCR Kit |
| Catalog Number | CSB-DKT031 |
| Physical Form | Liquid |
| Storage Conditions | -20 ±5°C, avoid repeated freeze-thaw cycles; can be stored at 2-8°C for frequent use |
| Transportation Conditions | ≤0°C; transport on dry ice |
| Product Components | Buffer system, dNTPs, Taq DNA Polymerase, UDG, M-MLV reverse transcriptase, RNase Inhibitor |
| Quality Control | All components have been tested and found to be free of nucleases, endonucleases, and residual RNases. |
| Shelf Life | 12 months |
Product Description
The One Step RT-qPCR Kit represents an advanced reagent kit designed for quantitative PCR reactions employing RNA as the template. Streamlining the experimental procedure, this kit facilitates both reverse transcription and quantitative PCR within the same reaction tube. The innovative approach eliminates complexities in the workflow. Leveraging the efficiency of heat-resistant M-MLV reverse transcriptase, the kit enables the synthesis of the first strand cDNA, while HS Taq DNA Polymerase drives quantitative amplification. Specifically tailored for systems utilizing fluorescent probes, it excels in one-step amplification scenarios.
Product Components
| Label | Components | Specifications |
|---|---|---|
| 50T | 2× One-step Buffer Mix | 0.75 mL |
| 100T | One Step RT-qPCR Taq Mix | 50 μL |
| 500T | 100 μL |
One Step RT-qPCR Kit: Streamlining Gene Expression Analysis
The One Step RT-qPCR Kit is a game-changing tool for gene expression analysis, seamlessly integrating reverse transcription (RT) and real-time quantitative PCR (qPCR) into a single, streamlined workflow. This innovative kit eliminates the need for multiple steps and reduces the risk of contamination, making it an ideal choice for researchers seeking a rapid, sensitive, and reliable method for quantifying gene expression levels.
Key Features of One Step RT-qPCR Kit:
- Integrated RT and qPCR: Combines RT and qPCR into a single step, saving time and reducing the risk of contamination.
- Exceptional Sensitivity: Detects as little as 0.5 pg of total RNA template, enabling quantification of low-abundance genes.
- High Specificity: Utilizes hot start Taq DNA polymerase to minimize non-specific amplification, ensuring accurate and reliable results.
- Easy to Use: Requires only template and primers, simplifying experimental setup and reducing the potential for errors.
- Fast and Efficient: Streamlined workflow minimizes reaction time and maximizes laboratory efficiency.
- Repeatable and Robust: Single-tube format and optimized buffer system ensure consistent performance across experiments.
Applications of One Step RT-qPCR Kit:
- Gene Expression Analysis: Quantify gene expression levels from a wide range of RNA samples, including cell cultures, tissues, and clinical specimens.
- Rapid Gene Cloning: Efficiently clone genes from RNA templates without the need for intermediate DNA purification steps.
- Colony PCR: Direct PCR amplification from bacterial colonies, enabling rapid screening and identification.
- Genotyping: RNA-based target identification combined with direct PCR amplification for streamlined genotyping procedures.
- TA Cloning: Streamlined TA cloning, integrating RT and PCR into a single step for efficient gene insertion into TA vectors.
Benefits of One Step RT-qPCR Kit:
- Saves time and effort: Streamlined workflow eliminates multiple steps, reducing hands-on time and increasing laboratory efficiency.
- Reduces contamination risk: Single-tube format minimizes sample exposure and prevents contamination during reaction setup.
- Enhances accuracy: Hot start Taq DNA polymerase ensures specific amplification, reducing background noise and improving data quality.
- Improves sensitivity: Detects low-abundance RNA targets, enabling the study of genes with weak expression.
- Increases reproducibility: Consistent results across experiments, ensuring reliable data interpretation.
Overall, the One Step RT-qPCR Kit is an invaluable tool for molecular biology research, offering a streamlined, sensitive, and reliable approach to gene expression analysis. Its ease of use, rapid performance, and wide range of applications make it an essential addition to any laboratory.
Additional Information:
- The One Step RT-qPCR Kit is compatible with a variety of real-time PCR instruments and thermocyclers.
- The kit is available in various formats to suit different experimental needs and sample volumes.
- Detailed protocols and technical support are available from the manufacturer.
Conclusion:
The One Step RT-qPCR Kit represents a significant advancement in gene expression analysis, providing researchers with a powerful tool to accurately quantify gene expression levels from a wide range of samples. Its exceptional performance, ease of use, and versatility make it an indispensable tool for any molecular biology laboratory.