In the realm of molecular biology, the synthesis of RNA through in vitro transcription is a fundamental technique with broad applications, from RNA labeling to the production of RNA probes. Hi-yield T7 In Vitro Transcription Reagent, enriched with N1-Me-pUTP, emerges as a beacon of excellence, promising not just transcription but transcription with enhanced precision and efficiency.
1. N1-Me-pUTP Enrichment
The hallmark of Hi-yield T7 is the incorporation of N1-Me-pUTP, a modified nucleotide that elevates the efficiency and fidelity of transcription. This modification enhances RNA stability and offers increased resistance to degradation, ensuring the synthesis of high-quality RNA transcripts.
2. Exceptional Yield and Purity
Hi-yield T7 stands true to its name, delivering exceptional yields of RNA transcripts without compromising on purity. The reagent's optimized formulation minimizes non-specific transcription products, providing researchers with a higher concentration of the desired RNA.
3. Broad Transcription Range
Whether you are transcribing short RNA sequences or long transcripts, Hi-yield T7 In Vitro Transcription Reagent adapts to your experimental needs. The reagent exhibits versatility in accommodating a broad range of templates, making it a go-to solution for diverse research applications.
4. Ease of Use
Designed with the end-user in mind, the reagent boasts a user-friendly formulation, streamlining the in vitro transcription process. Researchers can execute transcription reactions with ease, reducing hands-on time and increasing overall experimental efficiency.
1. Increased Sensitivity in Downstream Applications
The incorporation of N1-Me-pUTP enhances the sensitivity of downstream applications, such as RNA-protein interaction studies, RNA structural analysis, and the generation of RNA for functional assays. Researchers can trust Hi-yield T7 to provide transcripts that excel in diverse applications.
2. Reduced Background Noise
Hi-yield T7's optimized composition minimizes background noise, ensuring that the transcribed RNA is clean and pure. This feature is particularly crucial for applications where low background is essential, such as in RNA hybridization studies and microarray analyses.
3. Compatibility with Various Templates
Whether transcribing from plasmid DNA, PCR products, or synthetic DNA templates, Hi-yield T7 demonstrates exceptional compatibility. Researchers have the flexibility to choose the template that best suits their experimental design without compromising on transcription efficiency.
1. RNA Labeling and Probing
Hi-yield T7 is an ideal choice for researchers engaged in RNA labeling and probing studies, offering enhanced efficiency and precision for the synthesis of labeled RNA molecules.
2. In Vitro Translation
For experiments involving in vitro translation, Hi-yield T7 ensures the production of high-quality RNA transcripts that serve as reliable templates for protein synthesis.
3. Functional RNA Studies
In the realm of functional RNA studies, Hi-yield T7's N1-Me-pUTP enrichment provides researchers with a powerful tool to generate RNA transcripts for investigations into RNA function and structure.
Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP)
|Application||Production of large quantities of RNA for downstream applications, such as in vitro translation, RNA interference, and RNA sequencing|
|Advantages||High yield of RNA, high purity of RNA, easy to use|
|Kit contents||T7 RNA polymerase, NTP mix (including N1-Me-pUTP), reaction buffer, salts, and RNase inhibitor|
|Protocol||1. Mix the following components in a sterile tube:|
- Production of large quantities of RNA for downstream applications, such as in vitro translation, RNA interference, and RNA sequencing.
- Synthesis of modified RNAs, such as capped RNAs and biotinylated RNAs.
- Production of RNA for use in RNA therapeutics and vaccines.
- High yield of RNA: The kit is optimized to produce high yields of RNA, typically in the range of 150-200 μg per reaction.
- High purity of RNA: The transcribed RNA is pure and free of contaminating DNA and proteins.
- Easy to use: The kit is ready-to-use and does not require any specialized equipment or training.
The protocol for using the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP) kit is as follows:
- Mix the following components in a sterile tube:
- 1 μg of DNA template
- 10 μL of reaction buffer
- 1 μL of T7 RNA polymerase
- 1 μL of NTP mix (including N1-Me-pUTP)
- 1 μL of RNase inhibitor
- Incubate the reaction at 37°C for 30-60 minutes.
- Stop the reaction by adding EDTA to a final concentration of 10 mM.
- Purify the transcribed RNA using a suitable method, such as column chromatography or gel electrophoresis.
If the yield of RNA is low, it may be due to one of the following reasons:The DNA template is not of good quality.The DNA template is not properly linearized.The reaction is not incubated for long enough.The T7 RNA polymerase is not active.
To troubleshoot the yield of RNA, you can try the following:
- Use a different DNA template.
- Linearize the DNA template with a restriction enzyme.
- Increase the incubation time.
- Test the activity of the T7 RNA polymerase by transcribing a control template.
In the realm of molecular biology research, where efficiency and precision are paramount, Gentaur stands as the premier distributor, ensuring you receive the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP) with unparalleled speed and reliability.
Empowering researchers to achieve high-yield, high-quality RNA transcripts, the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP) from Gentaur stands as an indispensable tool for gene expression studies. This exceptional reagent features modified N1-Me-pUTP nucleotides that enhance transcription efficiency and reduce the risk of premature termination, resulting in significantly higher RNA yields compared to standard T7 transcription kits.
Gentaur understands that time is of the essence in scientific research. That's why we prioritize expedited delivery of the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP), ensuring you have the tools you need to conduct your experiments with minimal delay. Our streamlined distribution network and dedicated customer service team work tirelessly to get your reagent delivered promptly and securely, minimizing disruptions to your research workflow.
Beyond speedy delivery, Gentaur provides comprehensive support throughout your Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP) usage. Our team of experienced professionals is readily available to assist you with protocol optimization, troubleshooting, and interpretation of results, ensuring you can maximize the reagent's capabilities and achieve your research goals.
Partner with Gentaur and experience the difference that efficiency, precision, and unwavering support can make in your laboratory. With the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP) and Gentaur's expedited distribution, you're well-equipped to conduct groundbreaking research with confidence and speed.
Key benefits of partnering with Gentaur for the Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP):
- Unparalleled speed and reliability: Receive your reagent promptly and securely, minimizing disruptions to your research workflow.
- Expert technical support: Access a team of experienced professionals for guidance and troubleshooting.
- Comprehensive resources: Utilize a wealth of online resources, including protocols and FAQs.
- Commitment to quality: Experience the Gentaur difference, ensuring the highest quality reagents and services.