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How to use Nycodenz?
OptiPrep™ Application Sheet S47a
V18
S29.pdf
v33
S57
S10
The ideal density gradient medium for isolation of cells
Nycograde Polysucrose
iodixanol barrier
Polymorphprep
Isolation of viruses
Isolation of Macromolecules
Slide shows
Links to Axis-Shield Sites
Certificates
Events
Brochures and leaflets
Canine Brucella
Cell Culture Media
Blog
How to use Fractionation Syringe Filter ?
mNGS in clinical microbiology laboratories
Protocols
Protocl LAb
dsRNA ELISA kit
Rnase assay kit (Fluorescence)
N-Glycan Kit
UltraNuclease (EC 3.1.30.2)
Recombinant Trypsin Digestion 10X (with EDTA, without phenol red)
Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP)
DNase assay Kit (Fluorescence)
UltraNuclease
Trypsin Assay Kit(ELISA)
single cell sequencing
Illumina NGS
Principal Applications and Approaches Of NovaSeq 6000
iSeq 100
MGI SP 960
T7 RNA Polymerase
The NextSeq 2000 Sequencing System illumina
Vaccinia Capping Enzyme
mRNA BsaI / BspQI
RNase inhibitor
DNase I
Pyrophosphatase Inorganic (yeast)
Molecular Biology Reagents
Molecular Biology
NGS Library Prep
Protein & Immunity
Cell Biology
mRNA-Based Therapy
SDS-PAGE electrophoresis
DNA Electrophoresis
Precast Gel
Courses
Luminometer
NEST
Quantitative Fluorescent PCR Diagnostic Kits
Fluorescent Precision
FluoroProbe Analyzer
Appointment
Immunoassay
Tn5 Transposase
pG-Tn5 Transposome
pAG-Tn5 Transposase for CUT&Tag
pAG-Tn5 Transposome
CUT&Tag Assay Kit (pAG-Tn5) for illumina
Nuclease Precision
GMP T7
GMP DNase I
GMP RNase Inhibitor
GMP Inorganic Pyrophosphatase
GMP Vaccinia Capping Enzyme
GMP CapMTase
GMP PolyA
CRISPR-Cas13a Protein
LbCas12a Editor
AlkB Demethylase
M.CviPI Methyltransferase
M.SssI CpG Methyltransferase
Thermostable Polymerase
High-Fidelity Taq
High-Fidelity DNA Polymerase
High-fidelity thermostable DNA polymerase
High-Fidelity Rapid DNA Polymerase
Pfu II: The Pinnacle of PCR Fidelity
Hemo Taq: Blood-Borne PCR Perfection
Klen Taq: The Epitome of Fidelity in PCR
ARMS-Taq
Bst DNA Polymerase
Protease K
Liquid Protease Precision
MagBead Nucleic Acid Kit
M-MLV RTase
Taq Polymerase
High-fidelity Taq
Unified RT-qPCR
Streamlined Multiplexing
Polymerase Sentinel
Molecular Kit
Enzyme Toolkit for Precision Molecular Biology Applications
molecular biology system
Laboratory Accessories
DNA & Protein Tools and Kits
RNA Accessory Reagents
PCR Mixes & Kits
Dehydrated Culture Media
Biochemicals & Bioreagents
Enzymes
DNA reagents
Thermostable Taq
HS Taq: Enhanced PCR
Precise qPCR
Microfluidic Pipette
High-Performance Centrifuge
Urine Preservation Tube
cfDNA Blood Tube
CTB Tube
WestLumiOne
WestLumi plus
Blue HRP Substrate
Stripping Solution
Robust EzBlot
Versatile Transfer Buffer
High-Throughput
Membrane Blotting
Unlocking the Future of Cell Biology: Precision and Scalability in Digital Droplet Systems
Mini Submerge
Multi-Submerge
Advanced Submerge
Rapid and Accurate Diagnostic Test for Tuberculosis
Rapidly Detecting Human Metapneumovirus
RSV Neo
Influenza Diagnosis
MAC-Ab
RapidStrep
Mycapil
Electrophoresis
Peristaltic
Customization of Vectors
0
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Home
Shop
Products
Contact us
Distributors
News
How to use Nycodenz?
OptiPrep™ Application Sheet S47a
V18
S29.pdf
v33
S57
S10
The ideal density gradient medium for isolation of cells
Nycograde Polysucrose
iodixanol barrier
Polymorphprep
Isolation of viruses
Isolation of Macromolecules
Slide shows
Links to Axis-Shield Sites
Certificates
Events
Brochures and leaflets
Canine Brucella
Cell Culture Media
Blog
How to use Fractionation Syringe Filter ?
mNGS in clinical microbiology laboratories
Protocols
Protocl LAb
dsRNA ELISA kit
Rnase assay kit (Fluorescence)
N-Glycan Kit
UltraNuclease (EC 3.1.30.2)
Recombinant Trypsin Digestion 10X (with EDTA, without phenol red)
Hi-yield T7 in vitro transcription reagent (N1-Me-pUTP)
DNase assay Kit (Fluorescence)
UltraNuclease
Trypsin Assay Kit(ELISA)
single cell sequencing
Illumina NGS
Principal Applications and Approaches Of NovaSeq 6000
iSeq 100
MGI SP 960
T7 RNA Polymerase
The NextSeq 2000 Sequencing System illumina
Vaccinia Capping Enzyme
mRNA BsaI / BspQI
RNase inhibitor
DNase I
Pyrophosphatase Inorganic (yeast)
Molecular Biology Reagents
Molecular Biology
NGS Library Prep
Protein & Immunity
Cell Biology
mRNA-Based Therapy
SDS-PAGE electrophoresis
DNA Electrophoresis
Precast Gel
Courses
Luminometer
NEST
Quantitative Fluorescent PCR Diagnostic Kits
Fluorescent Precision
FluoroProbe Analyzer
Appointment
Immunoassay
Tn5 Transposase
pG-Tn5 Transposome
pAG-Tn5 Transposase for CUT&Tag
pAG-Tn5 Transposome
CUT&Tag Assay Kit (pAG-Tn5) for illumina
Nuclease Precision
GMP T7
GMP DNase I
GMP RNase Inhibitor
GMP Inorganic Pyrophosphatase
GMP Vaccinia Capping Enzyme
GMP CapMTase
GMP PolyA
CRISPR-Cas13a Protein
LbCas12a Editor
AlkB Demethylase
M.CviPI Methyltransferase
M.SssI CpG Methyltransferase
Thermostable Polymerase
High-Fidelity Taq
High-Fidelity DNA Polymerase
High-fidelity thermostable DNA polymerase
High-Fidelity Rapid DNA Polymerase
Pfu II: The Pinnacle of PCR Fidelity
Hemo Taq: Blood-Borne PCR Perfection
Klen Taq: The Epitome of Fidelity in PCR
ARMS-Taq
Bst DNA Polymerase
Protease K
Liquid Protease Precision
MagBead Nucleic Acid Kit
M-MLV RTase
Taq Polymerase
High-fidelity Taq
Unified RT-qPCR
Streamlined Multiplexing
Polymerase Sentinel
Molecular Kit
Enzyme Toolkit for Precision Molecular Biology Applications
molecular biology system
Laboratory Accessories
DNA & Protein Tools and Kits
RNA Accessory Reagents
PCR Mixes & Kits
Dehydrated Culture Media
Biochemicals & Bioreagents
Enzymes
DNA reagents
Thermostable Taq
HS Taq: Enhanced PCR
Precise qPCR
Microfluidic Pipette
High-Performance Centrifuge
Urine Preservation Tube
cfDNA Blood Tube
CTB Tube
WestLumiOne
WestLumi plus
Blue HRP Substrate
Stripping Solution
Robust EzBlot
Versatile Transfer Buffer
High-Throughput
Membrane Blotting
Unlocking the Future of Cell Biology: Precision and Scalability in Digital Droplet Systems
Mini Submerge
Multi-Submerge
Advanced Submerge
Rapid and Accurate Diagnostic Test for Tuberculosis
Rapidly Detecting Human Metapneumovirus
RSV Neo
Influenza Diagnosis
MAC-Ab
RapidStrep
Mycapil
Electrophoresis
Peristaltic
Customization of Vectors
+32 1 658 90 45
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Axis Shield
Plant Media
Antibodies
Affigen Inc.
Lymphoprep 500 ml
Isolate mononuclear cells from peripheral blood, cord blood, or bone marrow with Lymphoprep™—a cost-effective alternative to Ficoll-Paque™.
126.00
€
126.0
EUR
Nycodenz
Product name Nycodenz AG®
It’s a gradient medium derivate of benzoic acid with three aliphatic hydrophilic side chains.
372.00
€
372.0
EUR
OptiPrep 250mL
Simplify the isolation and purification of macromolecules; viruses; a wide range of cell types; and organelles such as nuclei, mitochondria, endosomes, or exosomes by using OptiPrep™ medium. This flexible, versatile, and gentle density gradient medium is non-ionic, iodixanol-based (60% w/v) and has a density of 1.320 ± 0.001 g/mL. OptiPrep™ has been used clinically in X-ray contrast imaging and has been shown to have lower toxicity when compared to ionic gradient compounds such as metrizamide.
172.00
€
172.0
EUR
Polymorphprep, 250 ml
Room temperature
Storage condition
Storage and Stability Conditions of Polymorphprep™
• Polymorphprep™ should be stored at room temperature (20°C).
• It’s stable for 3 years provided the solution is kept sterile and protected from light.
• Prolonged exposure to direct sunlight leads to release of iodine from the Sodium Diatrizoate molecule (this effect is negligible when working with the solution on a day to day basis.)
Brand Axis-shield
Polymorphprep is a type of density gradient medium that is used in laboratory settings to separate cells or other biological particles based on their size and density. It is commonly used in a technique called density gradient centrifugation, which involves spinning a tube of Polymorphprep and other components at high speeds to create a gradient of increasing density. This gradient allows cells or other particles to be separated based on their density and size, with heavier or denser particles settling towards the bottom of the tube and lighter or less dense particles remaining towards the top.
Polymorphprep is a specific brand of density gradient medium that is made by Sigma-Aldrich, a company that specializes in supplying chemical, biological, and pharmaceutical products to researchers and other professionals. It is commonly used in a variety of applications, including blood transfusion, tissue culture, and gene expression analysis. Polymorphprep is also used in research and diagnostic laboratories to purify or isolate cells or other biological materials, such as viruses or proteins.
Applications Isolation of human polymorphonuclear cells
Composition The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations:
• Sodium diatrizoate 13.8% (w/v)
• Dextran 500 8.0 (w/v)
Principle of the separation procedure
How Polymorphprep™ works
Polymorphonuclear leukocytes (PMNs) has a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than the mononuclear cells (higher than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a density barrier similar to that used for mononuclear cell. Several procedures have been developed to overcomes these issues.
The high osmolality of Polymorphprep™ causes erythrocytes to lose water and shrink, thus increasing their effective buoyant densities. This allows the dextran aggregated erythrocytes to sediment rapidly through the dense medium.
As the cells sediment further into the medium, the osmotic gradient between the medium and the erythrocytes declines. This means that water loss from the erythrocytes is greater at the top of the Polymorphprep™ and progressively decreases as they sediment further. A gradient of diatrizoate is formed within the density barrier. The PMNs band within this density gradient while the mononuclear cells remain at the sample/medium interface.
Advantages
Polymorphprep™ vs Ficoll-Paque
• Ficoll-Paque (GE Health Care) is not endotoxin tested at a base level and higher price
• Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.
Alternatives Polymorphprep™ can be a good substitute for Ficoll-Paque
143.00
€
143.0
EUR
Lymphoprep 250ml
Dr. Arne Bøyum reported in 1968 a simple and efficient method for the isolation of mononuclear cells from human blood. For more than 45 years, a commercial medium known as Lymphoprep has been widely used to isolate these cells.
78.00
€
78.0
EUR
InstantBlue
inst-novexin
155.00
€
155.0
EUR
PPM Plant Preservative Mixture
PPM is a type of Plant Preservative Mixture
sold by Maxanim in volunes of 100ml with the product code PPM.0100
95.00
€
95.0
EUR
PPM Plant Preservative Mixture - 30 mL
Plant Preservation Mixture (PPMTM) is a robust broad-spectrum biocide formulated for use in plant tissue culture. PPMTM targets bacteria and fungi in plant tissue culture growth media as well as contaminated tissue. It affects key enzymes in the Krebs cycle and in the Electron Transport Chain. Depending on the dose and the level of contamination, PPM is a biocidal component in plant culture medium. In addition, it may also function as a biostatic compound as a preventative measure. When diluted with plant growth media it is effective as a microbiocide (i.e. bactericide and fungicide) against non-human health pathogenic organisms component of liquid or semi-solid plant culture media.
https://maxanim.com/
Plant Preservative Mixture, also known as PPM, is a solution that is used to preserve plant tissues for scientific research or other purposes. It is typically made up of several chemicals that help to prevent the tissues from deteriorating or becoming contaminated during storage or transportation.
There are many different formulations of PPM, and the specific composition of the mixture can vary depending on the specific needs of the research or application. Common ingredients in PPM formulations include chemicals that help to inhibit the growth of microbes (such as antibiotics or antimicrobial agents), protect against oxidative damage (such as antioxidants), and maintain the structural integrity of the plant tissues (such as tissue fixation agents).
PPM is commonly used in research and diagnostic laboratories to preserve plant tissues for analysis or other purposes. It is also used in a variety of other applications, including horticulture and agriculture. It is important to follow the manufacturer's instructions and safety precautions when handling PPM, as some of the chemicals it contains may be toxic or hazardous.
PPMTM is effective for most seed baring plants - angiosperm, as well as gymnosperm,- however, it is not recommended for use in ferns, mosses, algae and aquatic plants. Optimization may be required to maximize potency. While PPM is an excellent tool in the prevention and elimination of culture contamination it is not a substitute for asceptic laboratory techniques and appropriate air handling systems are recommended.
95.00
€
95.0
EUR
Neusilin, 5 gram
85.00
€
85.0
EUR
AffiELISA® Human chemerin ELISA Kit
CAT# AFG-YBE-02412
Size: 96T
725.00
€
725.0
EUR
AffiELISA® Human cholecystokinin, CCK ELISA Kit
CAT# AFG-YBE-02436
Size: 96T
725.00
€
725.0
EUR
AffiELISA® Human Interleukin 1, IL-1 ELISA Kit
CAT#AFG-YBE-00001
Size: 96T
725.00
€
725.0
EUR
AffiAB® Rabbit Anti-Goat IgG (H&L) HRP
CAT# AFG-YBS-0012
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Duck IgY (H&L) HRP
CAT# AFG-YBS-0011
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Donkey IgG (H&L) HRP
CAT# AFG-YBS-0010
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Dog IgG (H&L) HRP
CAT# AFG-YBS-0009
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Chicken IgY (H&L) HRP
CAT# AFG-YBS-0008
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Cat IgG (H&L) HRP
CAT# AFG-YBS-0007
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Rabbit Anti-Bovine IgG (H&L) HRP
CAT# AFG-YBS-0006
Size: 100 µL
320.00
€
320.0
EUR
AffiAB® Goat Anti-Rabbit IgG (H&L) HRP
CAT# AFG-YBS-0005
Size: 100 µL
320.00
€
320.0
EUR
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