Tryptone Type I (Casitose) BactoBio: Empowering Bacterial Growth and Culture
Enhance your bacteriological culture media with the exceptional properties of Tryptone Type I (Casitose) BactoBio, a high-quality peptone designed to nourish and support a wide range of bacteria. This versatile peptone provides a rich source of essential amino acids, nitrogen, and energy, fostering robust bacterial growth and enabling effective culture.
Tryptone Type I (Casitose) BactoBio: The Essential Peptone for Effective Bacterial Growth
In the realm of bacteriology, the selection of appropriate culture media is crucial for successful bacterial cultivation and analysis. Among the essential components of these media, peptones play a pivotal role in providing the necessary nutrients for bacterial growth and proliferation. Tryptone Type I (Casitose) BactoBio stands out as a premier peptone, offering a wealth of benefits for bacteriological research.
A Rich Nutrient Source for Bacterial Growth
Tryptone Type I (Casitose) BactoBio is a peptone derived from casein, a milk protein, resulting in a highly nutritious substance for bacteria. This peptone is a rich source of essential amino acids, nitrogen, and energy, providing the building blocks and fuel necessary for bacterial growth and metabolism.
Exceptional Solubility and Compatibility
Tryptone Type I (Casitose) BactoBio exhibits excellent solubility in aqueous solutions, ensuring its uniform distribution throughout culture media. This property facilitates easy preparation of media and promotes consistent bacterial growth. Additionally, the peptone's compatibility with a wide range of antibiotics allows for selective culture of specific bacterial strains.
Versatile Applications in Bacteriological Culture Media
The versatility of Tryptone Type I (Casitose) BactoBio makes it a valuable component in various bacteriological culture media. It serves as a key ingredient in nutrient broths, providing the essential nutrients for the growth of a diverse range of bacteria. The peptone's effectiveness extends to selective media, where it aids in the selective proliferation of desired bacterial strains, while inhibiting the growth of unwanted microorganisms.
Enhanced Bacterial Growth and Culture
The incorporation of Tryptone Type I (Casitose) BactoBio into bacteriological culture media promotes robust bacterial growth and facilitates effective culture. The peptone's nutritional richness ensures that bacteria have the necessary resources to thrive, leading to healthy and abundant bacterial cultures.
A Cornerstone of Bacteriological Research
With its exceptional nutritional value, excellent solubility, and wide-ranging applications, Tryptone Type I (Casitose) BactoBio has established itself as an essential peptone in bacteriological research. Its ability to promote robust bacterial growth and facilitate effective culture makes it an invaluable tool for microbiologists and scientists working with bacteria.
Summary of Key Features
- Rich source of essential amino acids, nitrogen, and energy for bacterial growth
- Excellent solubility in aqueous solutions, ensuring uniform distribution in culture media
- Compatibility with a wide range of antibiotics for selective bacterial culture
- Versatile applications in nutrient broths, selective media, and differentiation media
- Promotes robust bacterial growth and facilitates effective culture
Tryptone Type I (Casitose) BactoBio stands as a testament to the importance of peptones in bacteriological research. Its ability to provide the necessary nutrients for bacterial growth and its versatility in various culture media make it an indispensable tool for microbiologists and scientists alike.
Tryptone Type I Specifications
| Property | Specification |
|---|---|
| Appearance (Colour) | Cream |
| Appearance (Form) | Free-flowing, homogeneous fine powder |
| Solubility (Colour) 2% aq. solution | Light to medium amber, clear |
| pH (25°C) | 6.2 - 7.2 |
| Stability (After Autoclaving) | Light to medium amber, clear |
| Loss on Drying | ≤ 5.0% |
| Nitrogen Content (N) | 12.0-13.5% |
| NaCl | < 5.0% |
| Ash | < 15% |
| Total Aerobic Microbial Count (Plate Count Method) | ≤ 10,000 cfu/gm |
| Indole Production (0.1%) | Escherichia coli ATCC 25922 - Growth: Good, Result: Positive |
| Fermentable Carbohydrates (2%) | Escherichia coli ATCC 25922 - Growth: Good, Result: Negative |
| Acetylmethylcarbinol Production | Enterobacter aerogenes ATCC13048 - Growth: Good, Result: Positive |
| Hydrogen Sulphide Production (1.0%) | Salmonella typhimurium ATCC 14028 - Growth: Good, Result: Positive |
| Cultural Response | Inoculate and incubate at 37°C for 18-24 hours with the following organisms: |
| Organism | Staphylococcus aureus ATCC 25922 - Inoculum (cfu): 10-100, Growth: Good |
| Organism | Escherichia coli ATCC 25922 - Inoculum (cfu): 10-100, Growth: Good |
Applications: Tryptone Type I is an enzymatic digest of milk protein, rich in amino nitrogen sources, and is commonly used in microbiological and fermentation media. Its specified properties make it suitable for a variety of applications in laboratory and industrial settings.
Tryptone Type I, also known as Casitose, is a peptone produced from casein, a milk protein. It is a yellow-brown, water-soluble powder with a slightly fishy odor. Tryptone Type I is a high-quality peptone that is commonly used in bacteriological culture media.
Composition
Tryptone Type I is a complex mixture of amino acids, peptides, and carbohydrates. The exact composition of Tryptone Type I varies depending on the manufacturer, but it typically contains approximately 70% amino acids, 25% carbohydrates, and 5% other compounds.
Applications
Tryptone Type I is a versatile peptone that is used in a wide variety of bacteriological culture media. It is a good source of nitrogen, essential amino acids, and energy for bacteria. Tryptone Type I is commonly used in:
- Nutrient broths: Tryptone Type I is a key ingredient in many nutrient broths, which are used to support the growth of a wide variety of bacteria.
- Selective media: Tryptone Type I can be used in selective media to inhibit the growth of unwanted bacteria while allowing the growth of desired bacteria.
- Differentiation media: Tryptone Type I can be used in differentiation media to distinguish between different species of bacteria based on their ability to utilize certain nutrients.
- Anaerobic media: Tryptone Type I can be used in anaerobic media to support the growth of anaerobic bacteria.
Benefits
Tryptone Type I offers several benefits for bacteriological culture media, including:
- High nutritional value: Tryptone Type I is a rich source of nitrogen, essential amino acids, and energy for bacteria.
- Good solubility: Tryptone Type I is a water-soluble powder that dissolves easily in media.
- Low toxicity: Tryptone Type I is generally non-toxic to bacteria.
- Compatible with a wide range of antibiotics: Tryptone Type I is compatible with a wide range of antibiotics, which allows for the selection of specific bacteria.
Storage and Handling
Tryptone Type I should be stored in a cool, dry place away from direct sunlight. It is hygroscopic, so it is important to keep it tightly sealed to prevent moisture absorption. Tryptone Type I has a shelf life of several years when stored properly.
Safety
Tryptone Type I is generally considered safe to handle. However, it is important to avoid contact with eyes, skin, and mucous membranes. In case of contact, flush with plenty of water. If swallowed, seek medical attention.
Tryptone Soya Agar, TSA
Soybean Casein Digest Agar (SCDA), also known as Tryptone Soya Agar (TSA), is a general-purpose nutrient agar medium used for the isolation and cultivation of a wide variety of fastidious and non-fastidious microorganisms. It is a versatile and widely used medium that supports the growth of a broad spectrum of bacteria, including both aerobic and facultative anaerobic species.
Composition and Properties
SCDA is composed of a combination of soybean meal digest, casein hydrolysate, agar, and sodium chloride. The soybean meal digest and casein hydrolysate provide a rich source of amino acids, peptides, and other nutrients that are essential for bacterial growth. Agar, a polysaccharide derived from red algae, serves as the gelling agent, providing a solid surface for bacterial colonies to form. Sodium chloride maintains the osmotic balance of the medium.
| Property | Specification |
|---|---|
| Appearance (Colour) | Light Yellow |
| Appearance (Form) | Free-flowing, homogeneous powder |
| Solubility | 40.00 gm/liter |
| Solubility before Autoclaving (Clarity) | Clear to slightly opalescent |
| Gel Strength (1.5% gel) | Firm, comparable with 1.5% gel |
| pH (25°C) | 7.3 ± 0.2 |
| Prepared Medium Appearance after Autoclaving (Clarity) | Clear to very slightly opalescent |
| Prepared Medium Appearance after Autoclaving (Colour) | Light Yellow (Note: If desired, add defibrinated blood. After addition of 5% sterile defibrinated blood: Cherry red, opaque.) |
| Cultural Response | Inoculate and incubate at 30 ± 2°C for 18-24 hours |
Organism: Candida albicans ATCC 10231
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Hemolysis |
|---|---|---|---|
| 10-100 | Good | Good | --- |
Organism: Staphylococcus aureus ATCC 25923
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate | Hemolysis |
|---|---|---|---|---|
| 10-100 | Good | Good | >=70.0% | Beta |
Organism: Streptococcus pyogenes ATCC 19615
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate | Hemolysis |
|---|---|---|---|---|
| 10-100 | Good | Good | >=70.0% | Beta |
Organism: Bacillus subtilis ATCC 6633
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate | Hemolysis |
|---|---|---|---|---|
| 10-100 | Good | Good | >=70.0% | None |
Organism: Bacteroides vulgatus ATCC 8482
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate | Hemolysis |
|---|---|---|---|---|
| 10-100 | Good | Good | >=70.0% | None |
Organism: Pseudomonas aeruginosa ATCC 27853
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate |
|---|---|---|---|
| 10-100 | Good | Good | >=70.0% |
Organism: Salmonella typhimurium ATCC 14028
| Inoculum (cfu) | Growth w/blood | Growth w/o blood | Recovery Rate |
|---|---|---|---|
| 10-100 | Good | Good | >=70.0% |
Applications: Used for the isolation and cultivation of both fastidious and non-fastidious microorganisms.
Composition:
| Ingredients | gm/lt. |
|---|---|
| Papaic digest of soybean meal | 15.00 |
| Agar | 15.00 |
| Pancreatic digest of casein | 15.00 |
| Sodium chloride | 5.00 |
Directions:
- Add 40.00 gm powder to 1.0 liter distilled/deionized water and mix thoroughly.
- Gently heat and bring to boiling.
- Autoclave at 15 psi pressure at 121°C for 15 minutes.