The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. Its tunable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with streamlined workflows. Configure the system to sequence a trio in one day or up to 48 genomes in ~2 days for comprehensive coverage.
Prep
3-5hr
Illumina DNA PCR-Free Prep
A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing.
llumina DNA Prep
A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.
Sequence
≤ 44hr
NovaSeq 6000 Reagent Kits v1.5
Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.
Analyze
<25min*
*Time per sample, assuming 200x coverage/sample
DRAGEN Germline Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)
The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both.
DRAGEN Somatic Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)
The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF.
Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq 6000 system offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell.
Prep
6hrs*
*2.5 days total prep time, 6 hours hands-on timeIllumina DNA Prep with Enrichment
This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.
Sequence
~25hrs
NovaSeq 6000 Reagent Kits v1.5
Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.
Analyze
<8min*
*Time per sample, assuming 100x coverage/sample
DRAGEN Enrichment
This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both.
Prep
~ 7hrs
Illumina Stranded Total RNA Prep
Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.
Sequence
~ 25hrs
NovaSeq 6000 Reagent Kits v1.5
Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.
Analyze
<15min*
*Time per sample, assuming 100x coverage/sample
DRAGEN RNA Pipeline
Performs alignment, quantification, and fusion detection.
Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. With the NovaSeq 6000 system, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell.