The NovaSeq 6000 system performs whole-genome sequencing efficiently and cost-effectively. Its tunable output generates up to 6 Tb and 20 billion reads in dual flow cell mode with streamlined workflows. Configure the system to sequence a trio in one day or up to 48 genomes in ~2 days for comprehensive coverage.

 


Prep

  3-5hr


Illumina DNA PCR-Free Prep

A high-performing, fast, and integrated workflow for sensitive applications such as human whole-genome sequencing or tumor-normal sequencing.

llumina DNA Prep

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species.

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Sequence

 ≤ 44hr 


NovaSeq 6000 Reagent Kits v1.5

Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.


Analyze

 <25min*

 *Time per sample, assuming 200x coverage/sample ​


DRAGEN Germline Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)

The DRAGEN Germline Pipeline v3 aligns and optionally variant calls FASTQ files, outputting a BAM, VCF, or both.

DRAGEN Somatic Pipeline (on BaseSpace Sequence Hub or on DRAGEN server)

The DRAGEN Somatic Pipeline aligns and variant calls Tumor-only or Tumor/Normal FASTQ files, outputting BAM(s) and a VCF.

Representing less than 2% of the genome, WES is a cost-effective alternative to WGS. The NovaSeq 6000 system offers sequencing of up to 500 exomes in a single run using a dual S4 flow cell.

 

Prep

  6hrs*

 *2.5 days total prep time, 6 hours hands-on time

Illumina DNA Prep with Enrichment

This kit uses a fast, user-friendly workflow. On-bead tagmentation chemistry is combined with a simplified, single hybridization protocol to reduce total workflow time.

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Sequence

 ~25hrs


NovaSeq 6000 Reagent Kits v1.5

Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.




Analyze

 <8min*

  *Time per sample, assuming 100x coverage/sample  ​


DRAGEN Enrichment

This app aligns and optionally variant calls FASTQ, BAM or CRAM files, outputting a BAM, VCF, or both.




Prep

~ 7hrs


Illumina Stranded Total RNA Prep

Rapid library preparation from a broad range of sample types for studying the coding and non-coding transcriptome with unparalleled study flexibility.


Sequence

  ~ 25hrs 


NovaSeq 6000 Reagent Kits v1.5

Ready-to-use, cartridge-based reagents with multiple flow cell configurations create a combination of scalable output and proven performance for reliable high-throughput sequencing.

Analyze

 <15min*

  *Time per sample, assuming 100x coverage/sample 


DRAGEN RNA Pipeline

Performs alignment, quantification, and fusion detection.

Detect coding and multiple forms of noncoding RNA in normal or low-quality samples. With the NovaSeq 6000 system, you can sequence up to 400 transcriptomes in a single run using a dual S4 flow cell.