Highlights of PrecisionPro™ Recombinant Trypsin Digestion 10X: Empowering High-Throughput Proteomic Analysis with Unmatched Efficiency

Noteworthy Aspects

1. Enhanced Digestion Efficiency

PrecisionPro™ Recombinant Trypsin Digestion 10X is engineered to deliver optimum digestion efficiency. Its recombinant nature ensures consistent and robust cleavage, providing researchers with the assurance that every sample will undergo thorough and precise digestion.

2. High Specificity

Crafted with a focus on high specificity, this 10X formulation of recombinant trypsin excels at cleaving peptide bonds at specific amino acid residues. This results in a more accurate representation of the proteome, with minimized nonspecific cleavage events.

3. Accelerated Workflows

Designed for high-throughput applications, PrecisionPro™ expedites proteomic sample processing. Researchers can achieve rapid digestion without compromising result quality, allowing for increased sample throughput in time-sensitive experiments.

4. Versatility in Sample Types

From complex biological matrices to purified protein samples, PrecisionPro™ Recombinant Trypsin Digestion 10X exhibits versatility in handling various sample types. This adaptability ensures that researchers can apply this enzymatic tool across a wide range of experimental setups.

Advantages

1. Consistent Performance

PrecisionPro™ guarantees consistent performance batch after batch, minimizing experimental variability and ensuring reproducible results for longitudinal studies and large-scale experiments.

2. Reduced Autolysis

The recombinant nature of the trypsin in this formulation significantly reduces autolysis, enhancing the stability of the enzyme and extending its shelf life. Researchers can confidently plan experiments without concerns about enzyme degradation.

3. Cost-Effective 10X Concentration

The 10X concentration of PrecisionPro™ Recombinant Trypsin Digestion minimizes the amount of enzyme required per reaction, resulting in cost savings for high-throughput laboratories without compromising on performance.

Applications

1. Shotgun Proteomics

PrecisionPro™ is an ideal choice for shotgun proteomics, enabling comprehensive analysis of complex protein mixtures with unparalleled speed and accuracy.

2. Quantitative Proteomics

For researchers engaged in quantitative proteomics, PrecisionPro™ Recombinant Trypsin Digestion 10X ensures precise and consistent cleavage for accurate quantification of proteins across samples.

3. Proteogenomics

In the evolving field of proteogenomics, PrecisionPro™ plays a crucial role by facilitating efficient and reliable protein digestion for subsequent analysis of protein-coding genes.

Comparison of Recombinant Trypsin Digestion 10X to Traditional Trypsin:

Troubleshooting:

If the cells are not dislodging after 5 minutes, it may be due to one of the following reasons:

• The cells are not properly attached to the dish.
• The trypsin solution is too old or has been diluted too much.
• The cells are resistant to trypsin digestion.

If you are having trouble dissociating the cells, you can try the following:

• Increase the incubation time up to 10 minutes.
• Use a fresh batch of trypsin solution.
• Try using a different type of trypsin, such as TrypLE Express.

Safety:

Recombinant Trypsin Digestion 10X is a hazardous material and should be handled with caution. It should be used in a fume hood and gloves should be worn when handling it. If the trypsin solution comes into contact with skin or eyes, rinse the affected area immediately with water and seek medical attention.

Applications of Recombinant Trypsin Digestion 10X: