In the fast-paced realm of proteomics, where precision and efficiency are paramount, PrecisionPro™ Recombinant Trypsin Digestion 10X emerges as a game-changer. This cutting-edge enzymatic solution is tailor-made for high-throughput proteomic analysis, offering researchers an unparalleled tool to accelerate workflows while maintaining the utmost precision in protein digestion.
Highlights of PrecisionPro™ Recombinant Trypsin Digestion 10X: Empowering High-Throughput Proteomic Analysis with Unmatched Efficiency
1. Enhanced Digestion Efficiency
PrecisionPro™ Recombinant Trypsin Digestion 10X is engineered to deliver optimum digestion efficiency. Its recombinant nature ensures consistent and robust cleavage, providing researchers with the assurance that every sample will undergo thorough and precise digestion.
2. High Specificity
Crafted with a focus on high specificity, this 10X formulation of recombinant trypsin excels at cleaving peptide bonds at specific amino acid residues. This results in a more accurate representation of the proteome, with minimized nonspecific cleavage events.
3. Accelerated Workflows
Designed for high-throughput applications, PrecisionPro™ expedites proteomic sample processing. Researchers can achieve rapid digestion without compromising result quality, allowing for increased sample throughput in time-sensitive experiments.
4. Versatility in Sample Types
From complex biological matrices to purified protein samples, PrecisionPro™ Recombinant Trypsin Digestion 10X exhibits versatility in handling various sample types. This adaptability ensures that researchers can apply this enzymatic tool across a wide range of experimental setups.
1. Consistent Performance
PrecisionPro™ guarantees consistent performance batch after batch, minimizing experimental variability and ensuring reproducible results for longitudinal studies and large-scale experiments.
2. Reduced Autolysis
The recombinant nature of the trypsin in this formulation significantly reduces autolysis, enhancing the stability of the enzyme and extending its shelf life. Researchers can confidently plan experiments without concerns about enzyme degradation.
3. Cost-Effective 10X Concentration
The 10X concentration of PrecisionPro™ Recombinant Trypsin Digestion minimizes the amount of enzyme required per reaction, resulting in cost savings for high-throughput laboratories without compromising on performance.
1. Shotgun Proteomics
PrecisionPro™ is an ideal choice for shotgun proteomics, enabling comprehensive analysis of complex protein mixtures with unparalleled speed and accuracy.
2. Quantitative Proteomics
For researchers engaged in quantitative proteomics, PrecisionPro™ Recombinant Trypsin Digestion 10X ensures precise and consistent cleavage for accurate quantification of proteins across samples.
In the evolving field of proteogenomics, PrecisionPro™ plays a crucial role by facilitating efficient and reliable protein digestion for subsequent analysis of protein-coding genes.
Maximizing Enzymatic Activity
Unveiling the Potency of Recombinant Trypsin Digestion 10X
Enhanced Performance and Purity
Exploring the Benefits of EDTA and Phenol Red-Free Solutions
Streamlined Proteolytic Processes
Simplifying Digestion Protocols with Recombinant Trypsin 10X
Precision in Cell Culture Applications
Achieving Consistent Results with Trypsin Digestion 10X and EDTA
Comparison of Recombinant Trypsin Digestion 10X to Traditional Trypsin:
|Feature||Recombinant Trypsin Digestion 10X||Traditional Trypsin|
|Gentleness||Gentle||Can be harsh on cells|
|EDTA||Contains EDTA||Does not contain EDTA|
|Phenol red||Does not contain phenol red||Contains phenol red|
Storage and Stability:
Recombinant Trypsin Digestion 10X should be stored at 2-8°C. It is stable for up to 12 months at this temperature.
Preparation of a Working Solution:
To prepare a working solution of Recombinant Trypsin Digestion 10X, dilute it 1:10 with culture medium. For example, to prepare 10mL of a working solution, dilute 1mL of Recombinant Trypsin Digestion 10X with 9mL of culture medium.
The incubation time for Recombinant Trypsin Digestion 10X will vary depending on the type of cells being dissociated. For adherent mammalian cells, the incubation time is typically 2-5 minutes at 37°C.
Monitoring Cell Dissociation:
To monitor cell dissociation, periodically aspirate the trypsin solution and observe the cells under a microscope. When the cells are detached from the dish, they will appear round and floating in the medium.
Neutralization of Trypsin Activity:
Once the cells are detached from the dish, the trypsin activity should be neutralized to prevent further cell lysis. This can be done by adding an equal volume of serum or culture medium to the cell suspension.
If the cells are not dislodging after 5 minutes, it may be due to one of the following reasons:
- The cells are not properly attached to the dish.
- The trypsin solution is too old or has been diluted too much.
- The cells are resistant to trypsin digestion.
If you are having trouble dissociating the cells, you can try the following:
- Increase the incubation time up to 10 minutes.
- Use a fresh batch of trypsin solution.
- Try using a different type of trypsin, such as TrypLE Express.
Recombinant Trypsin Digestion 10X is a hazardous material and should be handled with caution. It should be used in a fume hood and gloves should be worn when handling it. If the trypsin solution comes into contact with skin or eyes, rinse the affected area immediately with water and seek medical attention.
Applications of Recombinant Trypsin Digestion 10X:
|Cell dissociation for passaging||Passaging HEK 293 cells|
|Primary cell culture||Dissociating primary keratinocytes from skin|
|Cell harvesting||Harvesting CHO cells for protein production|
|Preparation of single cell suspensions for flow cytometry||Flow cytometric analysis of T cells|
|Preparation of single cell suspensions for cell sorting||Cell sorting of hematopoietic stem cells|
- Convenient to use: ready-to-use solution
- Gentle on cells: recombinant trypsin is less harsh than traditional trypsin preparations
- Contains EDTA to chelate calcium and magnesium ions, which are required for cell adhesion
- Does not contain phenol red, which can interfere with some downstream assays
- Cell dissociation for passaging, primary cell culture, and cell harvesting
- Preparation of single cell suspensions for flow cytometry, cell sorting, and other downstream applications