Pfu II High Fidelity DNA Polymerase: Unleash Precision in PCR
Experience the pinnacle of PCR fidelity with Pfu II High Fidelity DNA Polymerase, an exceptional enzyme renowned for its remarkable accuracy, efficiency, and convenience. Achieve error-free amplification of even the most challenging targets and streamline your downstream applications with blunt-ended PCR products. Elevate your molecular biology endeavors to new heights with Pfu II High Fidelity DNA Polymerase.
Pfu II High Fidelity DNA Polymerase: The Epitome of Precision in PCR
Introduction
In the realm of molecular biology, the polymerase chain reaction (PCR) stands as a cornerstone technique, enabling the amplification of minute quantities of DNA for diverse applications. However, the fidelity of PCR, a measure of its accuracy in replicating DNA sequences, can vary among different DNA polymerases. To address this critical aspect, Pfu II High Fidelity DNA Polymerase emerges as a game-changer, offering exceptional accuracy and efficiency for demanding PCR applications.
Unveiling the Power of Pfu II
Derived from the hyperthermophilic archaeon Pyrococcus furiosus, Pfu II High Fidelity DNA Polymerase embodies an exceptional enzyme with remarkable attributes. Its exceptional fidelity stems from its robust 3'-5' exonuclease activity, also known as proofreading activity. This intrinsic proofreading mechanism meticulously scrutinizes newly synthesized DNA, correcting any misincorporations and ensuring the highest level of accuracy.
Achieving Unparalleled Fidelity
Pfu II High Fidelity DNA Polymerase boasts an error rate of approximately 4.5 x 10^-5, significantly lower than that of conventional Taq DNA Polymerase. This remarkable fidelity translates into error-free amplification of even the most challenging templates, making Pfu II the ideal choice for PCR applications where accuracy is paramount.
Amplification Efficiency: Every Target Molecule Counts
Alongside its exceptional fidelity, Pfu II High Fidelity DNA Polymerase excels in amplification efficiency. Its remarkable speed of 1 kb/10s ensures rapid PCR reactions, enabling the efficient amplification of target DNA sequences up to 6 kb in length. Whether you're amplifying short or long DNA fragments, Pfu II High Fidelity DNA Polymerase consistently delivers high-yield amplifications, ensuring that every target molecule is captured and amplified with precision.
Streamlined Downstream Applications
A notable feature of Pfu II High Fidelity DNA Polymerase is its ability to generate blunt-ended DNA products. This eliminates the need for additional enzymatic modification prior to downstream applications, such as cloning and sequencing. With blunt-ended PCR products, you can seamlessly ligate DNA fragments into vectors and directly proceed with sequencing without the hassle of overhang modification.
A Versatile Tool for Diverse Applications
Pfu II High Fidelity DNA Polymerase finds wide-ranging applications in molecular biology, from gene cloning and mutation analysis to sequencing and diagnostic PCR. Its exceptional fidelity, efficiency, and convenience make it an indispensable tool for researchers seeking the highest level of accuracy in their PCR experiments.
Pfu II High Fidelity DNA Polymerase stands as a testament to scientific innovation, offering unparalleled accuracy, efficiency, and convenience in PCR. With its ability to generate error-free amplification of even the most challenging targets, Pfu II empowers researchers to tackle a wide range of molecular biology applications with confidence. As the epitome of precision in PCR, Pfu II High Fidelity DNA Polymerase is poised to revolutionize the field of molecular biology, paving the way for groundbreaking discoveries.
Introducing Pfu II High-Fidelity DNA Polymerase: A Beacon of Precision and Efficiency in PCR
Product Introduction
Emerging from the realm of genetic engineering, Pfu II High-Fidelity DNA Polymerase stands as a remarkable enzyme, meticulously crafted to redefine the standards of precision and efficiency in PCR. Derived from the renowned Pfu II DNA Polymerase, this advanced enzyme boasts superior thermostability, resulting from its recombinant expression in E. coli. Further enhanced by the fusion of a continuous capacity-enhancing structural domain, Pfu II High-Fidelity DNA Polymerase delivers exceptional amplification efficiency and fidelity, proving to be an invaluable tool for amplification of challenging templates.
Product Features: Unleashing the Power of Precision
- Exceptional Purity: With a purity exceeding 95%, Pfu II High-Fidelity DNA Polymerase ensures minimal interference from contaminants, guaranteeing the integrity of your PCR results.
- Unparalleled Fidelity: Surpassing the fidelity of Taq DNA Polymerase, Pfu II High-Fidelity DNA Polymerase minimizes error rates, ensuring the accuracy of your amplified DNA sequences.
- Enhanced Sensitivity: Strategically modified to enhance template affinity, Pfu II High-Fidelity DNA Polymerase elevates the detection sensitivity of low-concentration templates, enabling the amplification of even the faintest traces of DNA.
- Long-Fragment Amplification Prowess: Capable of amplifying λ-DNA target fragments up to 10 kb in length, Pfu II High-Fidelity DNA Polymerase proves its prowess in amplifying large DNA sequences.
Product Applications: A Versatile Tool for Diverse Molecular Biology Needs
- Long-Fragment PCR Amplification: Pfu II High-Fidelity DNA Polymerase excels in amplifying long DNA fragments, making it an indispensable tool for a wide range of molecular biology applications.
- Cloning of DNA Fragments: With its ability to generate high-fidelity DNA fragments, Pfu II High-Fidelity DNA Polymerase streamlines the process of cloning DNA fragments into vectors.
Product Data: Unveiling the Molecular Architecture
The SDS-PAGE electrophoresis image showcases the distinct bands corresponding to Pfu II High-Fidelity DNA Polymerase, providing visual confirmation of its purity and molecular integrity.
Amplification effect at different template concentrations
Comparison test with competitive enzymes
A Paradigm Shift in PCR
Pfu II High-Fidelity DNA Polymerase represents a significant leap forward in PCR technology, offering a unique blend of exceptional fidelity, efficiency, and versatility. By empowering researchers to tackle even the most challenging PCR applications with confidence, Pfu II High-Fidelity DNA Polymerase paves the way for groundbreaking discoveries and advancements in the field of molecular biology.
| Property | Value |
|---|---|
| Product Name | Pfu II High-Fidelity DNA Polymerase |
| Source | Recombinant expression in E. coli |
| Catalog Number | CSB-DEM043 |
| Physical Form | Liquid |
| Enzyme Activity | 1 U/μL |
| Storage Conditions | -20 ±5°C |
| Storage Buffer | 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween20, 0.5% NP-40, 50% Glycerol |
| Activity Definition | The amount of enzyme required to incorporate 10 nmol of deoxynucleotides into acid-insoluble material in 30 minutes at 75°C is defined as 1 unit (U). |
| Quality Control | No detectable exonuclease and endonuclease activities |
| Shelf Life | 24 months |
Pfu II High-Fidelity DNA Polymerase: Elevating PCR to New Heights of Precision and Efficiency
In the realm of molecular biology, the polymerase chain reaction (PCR) stands as an indispensable technique, enabling the amplification of minute quantities of DNA for a vast array of applications. However, the fidelity of PCR, a measure of its accuracy in replicating DNA sequences, can vary considerably among different DNA polymerases. To address this critical aspect, Pfu II High-Fidelity DNA Polymerase emerges as a game-changer, offering exceptional accuracy, efficiency, and convenience for demanding PCR applications.
A Fusion of Precision and Speed
Pfu II High-Fidelity DNA Polymerase embodies an exceptional enzyme, meticulously crafted through the fusion of a proofreading domain and an enhanced processivity domain. This synergistic combination results in a remarkable enzyme that delivers both increased fidelity and speed, empowering researchers to achieve error-free amplification in a time-efficient manner.
Standalone Enzyme or Master Mix Format
Pfu II High-Fidelity DNA Polymerase provides the flexibility to be utilized as either a standalone enzyme or in a convenient master mix format. This versatility allows researchers to tailor their PCR protocols according to their specific needs and preferences.
Highly Specific Amplification of Diverse Templates
Pfu II High-Fidelity DNA Polymerase excels in amplifying a wide range of templates, exhibiting remarkable specificity even with challenging targets. Its ability to accurately amplify diverse DNA sequences makes it an invaluable tool for a broad spectrum of PCR applications.
An Ideal Choice for Cloning and Long/Challenging Amplicons
Pfu II High-Fidelity DNA Polymerase stands out as an ideal choice for cloning applications, generating high-fidelity DNA fragments that seamlessly integrate into vectors. Additionally, it proves its prowess in amplifying long or challenging amplicons, enabling the successful amplification of DNA sequences that may pose difficulties for other DNA polymerases.
Surpassing Expectations with Stable Performance and Reduced Enzyme Usage
Compared to conventional DNA polymerases, Pfu II High-Fidelity DNA Polymerase delivers exceptional stability, ensuring consistent and reliable performance across a range of experimental conditions. Moreover, it requires significantly lower enzyme concentrations, leading to reduced costs and increased cost-effectiveness.
Shorter Experimental Protocols and Higher Yield
Pfu II High-Fidelity DNA Polymerase streamlines PCR workflows, enabling shorter experimental protocols and higher yield. This translates into faster turnaround times and greater efficiency in DNA amplification.
Pfu II High-Fidelity DNA Polymerase: The Epitome of PCR Excellence
Pfu II High-Fidelity DNA Polymerase represents a paradigm shift in PCR technology, offering a unique blend of exceptional fidelity, efficiency, and convenience. By empowering researchers to tackle even the most demanding PCR applications with confidence, Pfu II High-Fidelity DNA Polymerase paves the way for groundbreaking discoveries and advancements in the field of molecular biology.
Embrace the power of Pfu II High-Fidelity DNA Polymerase and elevate your PCR experiments to new heights of precision, efficiency, and productivity.
| No. | Component Name | Specifications |
|---|---|---|
| 1 | 5× Pfu buffer | 0.6 mL, 3 mL, 6 mL |
| 2 | Pfu II High-Fidelity DNA Polymerase | 100 U, 500 U, 1000 U |