M-MLV Reverse Transcriptase: A Versatile Enzyme for cDNA Synthesis

M-MLV Reverse Transcriptase is an efficient and widely used enzyme for cDNA synthesis from RNA templates. It features high processivity and fidelity, making it suitable for a wide range of applications, including gene expression analysis, cloning, and sequencing.

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M-MLV Reverse Transcriptase: An Enzyme for cDNA Synthesis

M-MLV Reverse Transcriptase (RTase) is an enzyme derived from the Moloney murine leukemia virus (M-MLV) that catalyzes the synthesis of complementary DNA (cDNA) from RNA templates. It is a versatile and widely used enzyme in molecular biology research due to its high processivity, fidelity, and ability to handle long RNA molecules.

Properties and Applications

M-MLV RTase possesses several key properties that make it a valuable tool for cDNA synthesis:

  1. High Processivity: The enzyme can efficiently elongate cDNA strands, synthesizing long stretches of DNA without pausing or detaching from the RNA template.
  2. High Fidelity: M-MLV RTase incorporates nucleotides accurately, minimizing errors during cDNA synthesis. This fidelity is crucial for maintaining the integrity of the genetic information being copied.
  3. Ability to Handle Long RNA Molecules: Unlike some other RTases, M-MLV RTase can effectively reverse transcribe long RNA molecules, making it suitable for cDNA synthesis from a wide range of RNA sources.
  4. Thermostability: M-MLV RTase is relatively stable at elevated temperatures, allowing for cDNA synthesis under mild conditions.

M-MLV RTase has a broad range of applications in molecular biology research, including:

  1. Gene Expression Analysis: cDNA synthesized using M-MLV RTase can be used to quantify gene expression levels using techniques such as real-time PCR or qPCR.
  2. Cloning: cDNA generated with M-MLV RTase can be inserted into cloning vectors for gene cloning and expression studies.
  3. Sequencing: cDNA derived from M-MLV RTase can be used for DNA sequencing to determine the nucleotide sequence of genes or transcripts.
  4. Synthesis of cDNA Libraries: M-MLV RTase is a key component in the construction of cDNA libraries, which represent the entire mRNA population of a cell or organism.

Advantages over Other RTases

M-MLV RTase offers several advantages over other RTases commonly used for cDNA synthesis:

  1. Higher Processivity: Compared to AMV RTase, M-MLV RTase can synthesize longer cDNA strands without disengaging from the RNA template.
  2. Reduced RNase H Activity: Unlike some other RTases, M-MLV RTase has minimal RNase H activity, which helps preserve the integrity of the RNA template during cDNA synthesis.
  3. Tolerance to Impurities: M-MLV RTase is relatively tolerant of impurities that may be present in RNA samples, making it less susceptible to inhibition.
  4. Availability of Modified Versions: Modified versions of M-MLV RTase, such as RNase H-deficient and thermostable variants, are available for specific applications.

M-MLV Reverse Transcriptase is a versatile and powerful enzyme that has become an essential tool in molecular biology research. Its high processivity, fidelity, ability to handle long RNA molecules, and relatively low RNase H activity make it a preferred choice for cDNA synthesis in a wide range of applications. With its well-established properties and extensive track record, M-MLV RTase is likely to continue playing a significant role in molecular biology research for years to come.


Product Overview

MMLV Reverse Transcriptase is an enzyme derived from the Moloney murine leukemia virus (M-MLV) that has been engineered through mutation screening to exhibit enhanced performance and stability. Unlike conventional M-MLV RTases, this modified version offers superior thermostability, enabling efficient reverse transcription at elevated temperatures of 50-60°C. This high-temperature tolerance effectively eliminates the adverse effects of RNA secondary structures and non-specific factors on cDNA synthesis, resulting in more robust and reliable cDNA generation.

Key Features
  • High Thermostability: Performs reverse transcription at high temperatures (50-60°C), ensuring stable reaction conditions.
  • Enhanced Reverse Transcription Efficiency: Guarantees efficient cDNA synthesis and high success rates, even with low-concentration RNA templates.
  • First-strand cDNA synthesis for RT-PCR and RT-qPCR: Generates high-quality cDNA from RNA templates for downstream applications such as real-time PCR and quantitative PCR.
  • Synthesis of cDNA for cloning and expression studies: Produces cDNA suitable for gene cloning and expression studies, enabling the study of gene function and protein production.
Product Comparison

SDS-PAGE electrophoresis analysis demonstrates the purity and integrity of MMLV Reverse Transcriptase compared to similar products. The enzyme exhibits a single distinct band at the expected molecular weight, indicating its high purity and lack of contaminating proteins.

Comparative testing with competitive enzymes


Accelerated thermal stability

Product Specification

Product NameM-MLV Reverse Transcriptase
SourceRecombinant expression in Escherichia coli
Catalog NumberCSB-DEM025
Physical FormLiquid
Enzyme Activity200 U/µL
Storage Conditions-20 ±5°C
Molecular Weight74 kDa
Storage Buffer20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% NP-40, 50% Glycerol
Activity Definition1 unit (U) is the amount required to incorporate 1 nmol of dTTP into acid-insoluble material in a 50 μL reaction system with Poly(rA)•Oligo(dT)15 as the template at 37°C for 10 minutes.
Quality ControlNo detectable endonuclease, DNase, and RNase activities. PCR testing shows no residual host genomic DNA.
Shelf Life24 months

Product Description

Elevate your reverse transcription with M-MLV Reverse Transcriptase, a meticulously engineered enzyme that not only reduces RNase H activity but also enhances thermal stability. Compared to wild-type M-MLV, this variant can synthesize the first strand cDNA at elevated temperatures, showcasing its versatility and adaptability. It operates with normal activity at 50-60°C, offering increased specificity, higher yield, and the remarkable ability to synthesize cDNA up to 12 kb in length.

Product Components
Component No.Component NameSpecifications
15× First-Strand Buffer0.4 mL, 2 mL, 4 mL
2M-MLV Reverse Transcriptase20000 U, 100000 U, 200000 U