UltraNuclease / Benzonase: A Powerful Tool for Nucleic Acid Purification

UltraNuclease / Benzonase is a highly purified endonuclease enzyme that can be used to efficiently and completely degrade DNA and RNA. It is a valuable tool for nucleic acid purification and preparation, and it is widely used in molecular biology research and clinical applications.

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Advanced Nuclease Technology: Unleashing Precision in Nucleic Acid Removal

Omnipotent Nuclease, renowned as a non-restrictive and broad-spectrum enzymatic solution, exhibits unparalleled efficacy in cleaving at any nucleotide position within the chain. This genetic engineering marvel, expressed and purified in E. coli under GMP conditions, offers a dynamic approach to digesting various forms of DNA and RNA. Whether dealing with double-stranded or single-stranded nucleic acids, linear or circular configurations, natural or denatured states, Omnipotent Nuclease excels under diverse conditions. Its versatility finds widespread utility in scientific research, where it efficiently reduces viscosity in cell supernatants and lysates, thereby enhancing protein purification and functional studies.

Beyond the realms of research, Omnipotent Nuclease emerges as a crucial tool in industries such as virus purification, vaccine production, and pharmaceuticals. Functioning as a potent host residual nucleic acid removal reagent, it diminishes host nucleic acids to picogram levels, elevating the efficacy and safety of biological products. Moreover, in cell therapy and vaccine research, Omnipotent Nuclease plays a pivotal role in averting the aggregation of peripheral blood mononuclear cells (PBMC), thereby contributing to the advancement of these critical fields.

Product Highlights:
Crafted with Precision: 

Engineered under the exacting standards of Good Manufacturing Practice (GMP), our product stands as a testament to uncompromising quality and reliability. Every step in its production adheres rigorously to the principles and guidelines set forth by GMP, ensuring a final product of the highest caliber.

Elevating Research Standards:

Our product finds its niche in the critical domain of nucleic acid removal, where its application goes beyond mere procedural steps. By implementing our solution, researchers can substantially enhance the outcomes of their investigations. The meticulous removal of nucleic acids not only refines research protocols but also contributes significantly to the safety profile of the end product. In the intricate landscape of scientific inquiry, where precision and purity are paramount, our product stands as a dependable ally for achieving superior research standards.

Product Name: UltraNuclease (GMP grade)

SourceRecombinant expression in Escherichia coli
Catalog NumberCSB-DEM077
Physical FormLiquid
Enzyme Activity250-300 U/μL
Storage Conditions-20±5°C
Storage Buffer20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol
Activity DefinitionThe amount of enzyme required to cause a change of 1.0 in ΔA260 absorbance value within 30 minutes in a 2.625 mL reaction system at 37°C, pH 8.0, equivalent to complete digestion of 37 μg salmon sperm
Shelf Life24 months

Product Overview:

UltraNuclease, an exceptional non-specific endonuclease, is synonymous with broad-spectrum nucleases. Isolated from Serratia marcescens, this enzymatic marvel exhibits unparalleled efficacy in cleaving between any nucleotides within DNA or RNA chains. The digestion process results in the formation of 2-5 base length 5'-monophosphate oligonucleotides. Operating seamlessly across diverse DNA and RNA forms, including double-stranded, single-stranded, linear, circular, natural, or denatured, UltraNuclease stands as a cornerstone in nucleic acid removal from biological products. Genetically engineered and meticulously expressed in Escherichia coli (E. coli), this product attains a purity level of ≥ 99%, ensuring optimal performance.

Professional Insights:

 UltraNuclease's robust capabilities extend to reducing the viscosity of cell supernatants and lysates, thereby enhancing efficiency in protein purification and functional studies. Notably, it plays a crucial role in preventing the clustering of peripheral blood mononuclear cells (PBMC) in the realms of cell therapy and vaccine research. The purity level of ≥ 99% underscores its suitability for applications demanding precision and reliability.


Product Components:

1UltraNuclease (GMP grade)25KU, 125KU, 250KU