RNA Mini Spin Column Set: A Comprehensive Solution for High-Purity RNA Purification
Unleash the power of gene expression analysis and cDNA synthesis with the RNA Mini Spin Column Set, your gateway to high-quality RNA purification from diverse biological samples. Experience the efficiency, convenience, and broad compatibility of this innovative silica membrane-based kit.
Product Overview
The RNA Mini Spin Column Set (2ml SC w/ CT each) is a versatile and reliable tool for purifying high-quality RNA from various biological samples. This comprehensive kit utilizes silica membrane technology to effectively capture and purify RNA molecules, providing a rapid and efficient method for gene expression studies, cDNA synthesis, and other RNA-based applications.
Key Features
- Exceptional RNA Purity: The silica membrane effectively removes contaminants, including proteins, DNA, and salts, resulting in high-quality RNA suitable for downstream applications.
- High Yield: The kit efficiently purifies RNA from a wide range of sample types, ensuring maximum recovery of RNA molecules.
- Fast and Convenient Protocol: The streamlined protocol minimizes hands-on time and allows for processing multiple samples simultaneously.
- Broad Compatibility: The kit is compatible with a variety of biological samples, including animal tissues, cultured cells, and plant tissues.
Applications
- Gene Expression Studies: Purify RNA for downstream applications such as qPCR, RT-PCR, and Northern blotting.
- cDNA Synthesis: Generate high-quality cDNA templates for various molecular biology techniques.
- RNA Sequencing: Prepare RNA samples for next-generation RNA sequencing analysis.
Kit Components
- 2ml Spin Columns (SC)
- 2ml Collection Tubes (CT)
- RNA Binding Buffer
- Washing Buffer
- RNase-Free DNase I
- Elution Buffer
Storage Conditions
Store the kit components at room temperature (15-25°C) for long-term storage. The RNase-Free DNase I should be stored at -20°C for extended stability.
Product Specifications
- Column Capacity: 20µg of total RNA
- Binding Capacity: 100µg of total RNA
- Processing Time: Approximately 30 minutes per sample
- Elution Volume: 50-100µl of Elution Buffer
Ordering Information
- Product Code: RNA Mini Spin Column Set (2ml SC w/ CT each)
- Available Package Sizes: 50, 100, 250, 500, and 1000 reactions
Please contact us for pricing and availability.
The Nucleic Acid Purification Kit features circular columns and collection tubes designed for the efficient purification and extraction of high-quality nucleic acids. Utilizing a silica membrane in column form, this kit ensures compatibility with both in-house and store-bought buffers. To eliminate the risk of nuclease contamination, nucleic acids are incubated in the tubes at 37°C for 1 hour. The recommended absorbance range for optimal results is 20-40µg. The entire set is supplied with 2ml collection tubes, and the stability of the kit is maintained when stored as per the recommended guidelines. This kit serves as a reliable and versatile tool for nucleic acid purification processes.
Property | Specification |
---|---|
Appearance (Form) | Circular Columns and Collection Tubes |
Absence of Nucleases | Nucleic acids incubated at 37°C for 1 hour |
Absorbance | 20-40µg |
Stability | Stable if stored as recommended |
RNA Mini Spin Column Set: Streamlined RNA Isolation for Molecular Research
Overview: The RNA Mini Spin Column Set stands as an indispensable tool for molecular biologists, providing a streamlined and efficient solution for the isolation and purification of high-quality RNA. Designed for versatility, scalability, and ease of use, this set offers researchers a reliable method to extract RNA from diverse biological samples.
Key Features:
- Spin Column Technology:
- Rapid RNA purification through centrifugation, ensuring a straightforward and time-efficient workflow.
- High Yield and Purity:
- Consistently delivers high yields of pure RNA, free from contaminants, making it suitable for a range of downstream applications.
- Versatility:
- Accommodates various starting materials, including cells, tissues, and blood, catering to the diverse needs of molecular biology experiments.
- Simple Workflow:
- User-friendly protocol with easy-to-follow steps, making RNA extraction accessible for both novice and experienced researchers.
- Optimized Binding Conditions:
- Columns designed with optimized binding conditions for selective and efficient RNA capture.
- Elution Flexibility:
- Facilitates RNA elution in a small volume, concentrating purified RNA for downstream applications like RT-PCR and qPCR.
- Scalability:
- Suited for both small-scale experiments and high-throughput workflows, ensuring consistent results across varying sample volumes.
- Quality Control:
- Manufactured under stringent quality control standards to ensure batch-to-batch consistency, reliability, and the preservation of RNA integrity.
Additional Benefits:
- RNA Stability:
- Preserves RNA stability during extraction, minimizing the risk of degradation for accurate downstream analyses.
- Inhibitor Removal:
- Efficient removal of potential inhibitors, enhancing the purity of the isolated RNA.
- Quality RNA for Various Applications:
- Suitable for a broad range of applications, including RT-PCR, Northern blotting, microarray analysis, and RNA sequencing.
- Time-Efficient Protocols:
- Designed for time-efficient RNA extraction, ideal for time-sensitive experiments and high-throughput settings.
- Compact Design:
- Compact design for efficient use of bench space and convenient storage.
- Cost-Effective:
- Provides cost-effective RNA isolation without compromising quality.
- Compatibility with Downstream Analysis Kits:
- Compatible with a variety of downstream analysis kits, ensuring seamless integration into existing experimental workflows.
- User Support and Documentation:
- Comprehensive user manuals and technical support for a confident and successful RNA extraction experience.
- Consistent Performance:
- Rigorous quality assurance measures for consistent and reproducible RNA isolation results.
In summary, the RNA Mini Spin Column Set offers researchers a robust, user-friendly, and cost-effective solution for high-quality RNA isolation, supporting a wide array of molecular biology applications and contributing to the advancement of scientific research.
Storage:
The product is recommended to be stored at room temperature, ensuring optimal conditions for its stability and efficacy.
Shelf Life:
The product has a shelf life of 36 months from the date of manufacture, providing an extended duration of reliability.
Transportation Information:
The product is not regulated for transport according to the IMDG (International Maritime Dangerous Goods) classification, confirming its non-hazardous nature during transportation.
HSN Code:
- For 100 units: 38229090 (GST 12%)
- For 50 units: 38229090 (GST 12%)
The RNA Mini Spin Column Set is an essential laboratory tool designed to streamline and simplify the isolation and purification of high-quality RNA molecules from various biological samples. This versatile set is widely utilized in molecular biology and genetic research applications, providing researchers with a reliable and efficient method for extracting RNA with minimal contamination.
Key Features:
- Spin Column Technology:
- The RNA Mini Spin Column Set operates on the principle of spin column technology, allowing for rapid and convenient RNA purification through centrifugation.
- High Yield and Purity:
- Engineered to deliver consistently high yields of pure RNA, the set effectively removes contaminants such as genomic DNA, proteins, and inhibitors, ensuring the obtained RNA is suitable for downstream applications.
- Versatility:
- Compatible with a diverse range of starting materials, including cultured cells, tissues, blood, and other biological samples, the RNA Mini Spin Column Set accommodates the needs of various experimental setups.
- Simple Workflow:
- The user-friendly protocol involves a few easy-to-follow steps, making the RNA extraction process straightforward and accessible for both experienced researchers and those new to molecular biology techniques.
- Optimized Binding Conditions:
- The columns are designed with optimized binding conditions to selectively capture RNA molecules, promoting efficiency and specificity in the isolation process.
- Elution Flexibility:
- The set allows for elution of RNA in a small volume of elution buffer, concentrating the purified RNA and facilitating its use in downstream applications such as reverse transcription and quantitative PCR.
- Scalability:
- Whether processing a small number of samples or working with high-throughput workflows, the RNA Mini Spin Column Set is designed for scalability, providing consistent results across varying sample volumes.
- Quality Control:
- Manufactured under stringent quality control standards, each set undergoes rigorous testing to ensure batch-to-batch consistency, reliability, and the preservation of RNA integrity.
The RNA Mini Spin Column Set is an indispensable tool for researchers working in molecular biology, genetics, and related fields, offering a rapid and efficient solution for obtaining high-quality RNA for applications ranging from gene expression analysis to functional genomics studies. With its simplicity, versatility, and reliability, this spin column set contributes to advancing research by enabling researchers to obtain pure and intact RNA with ease.