Unlocking Genomic Potential: Comprehensive Guide to pG-Tn5 Transposome

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Introducing pG-Tn5 Transposase

Innovative Fusion for Unparalleled Efficiency

pG-Tn5 Transposase is an advanced fusion of Protein G and a highly active mutant form of Tn5 transposase. This unique product integrates the functionalities of Tn5 transposase and Protein G, presenting a remarkable leap forward in genomic research capabilities. The mutant Tn5 transposase within this complex demonstrates an outstanding 1000-fold increase in in vitro transposition efficiency compared to the wild type.

Key Product Features

  • Dual Functionality: Possesses both the functions of Tn5 transposase and Protein A/Protein G.
  • Enhanced Efficiency: Exhibits a remarkable 1000 times higher in vitro transposition efficiency than the wild type.

Versatile Applications for Cutting-Edge Research

This groundbreaking product finds its utility across various genomic research applications, providing a versatile toolkit for scientists and researchers.

Product Applications:

  • Second-generation Sequencing Library Construction
  • ATAC-seq (Assay for Transposase-Accessible Chromatin using Sequencing)
  • CUT&Tag (Cleavage Under Targets & Tagmentation)

Why Choose pG-Tn5 Transposase?

In comparison to the conventional ChIP-seq method for protein-genome interaction research, CUT&Tag offers distinct advantages. Its shortened experimental cycle, high signal-to-noise ratio, excellent repeatability, and minimal cell input requirement make it particularly well-suited for cutting-edge studies in early embryonic development, stem cells, tumors, and epigenetics.

Elevate your genomic research with the unparalleled capabilities of pG-Tn5 Transposase – a catalyst for advancements in molecular exploration.

Product Components

Unlocking Precision with Unique Labeling

Embark on a journey of precision with our meticulously crafted label components. Each label is a testament to our commitment to excellence in molecular exploration.

Distinctive Label Components:
1pG-Tn5 Transposome (4 µM)12µL
Why Choose Our Labels?

Our labels go beyond conventional standards, delivering uniqueness in every component. Precision, efficiency, and innovation converge to redefine your molecular studies. Elevate your experiments with labels designed for excellence

Product Manual

Protein G-Tn5 is a hybrid protein that combines the features of Protein G and Tn5 transposase, offering a versatile and powerful tool in molecular biology applications. Protein G, known for its high affinity to immunoglobulins, is often used in antibody-related experiments, while Tn5 transposase is utilized for its ability to catalyze the precise insertion of DNA sequences into target genomes.

The fusion of Protein G and Tn5 transposase capitalizes on the strengths of both components. This chimeric protein maintains the antibody-binding capability of Protein G, allowing for efficient and specific capture of antibody-DNA complexes. Simultaneously, the Tn5 transposase portion facilitates the targeted integration of the captured DNA fragments into a host genome, enabling streamlined and controlled genetic manipulations.

Protein G-Tn5 finds applications in various molecular biology techniques, including chromatin immunoprecipitation sequencing (ChIP-seq), ATAC-seq (Assay for Transposase Accessible Chromatin with high-throughput sequencing), and other genomic studies. By combining the antibody recognition capabilities of Protein G with the precision of Tn5 transposase-mediated DNA integration, this hybrid protein simplifies experimental workflows and enhances the efficiency of genetic and epigenetic research. Researchers can leverage Protein G-Tn5 to gain insights into gene regulation, chromatin accessibility, and other aspects of genome dynamics with greater accuracy and reproducibility.

Product NamepG-Tn5 Transposome
SourceRecombinant expression in Escherichia coli
Catalog NumberCSB-DEM069
Physical FormLiquid
Storage Conditions-20°C
Molecular Weight70 kDa
Storage Buffer50mM HEPES (pH 7.2), 100mM NaCl, 0.1mM EDTA, 1mMDTT, 0.1% Triton X-100, 50% (v/v) Glycerol
Quality ControlNo residual nucleases and exonucleases
Shelf Life12 months

Revolutionizing Genomic Research: Unveiling pG-Tn5 Transposase

Innovative Fusion for Precision Genome Studies

Introducing pG-Tn5 Transposase, an ingenious fusion enzyme meticulously crafted by marrying Protein G with a modified, high-activity Tn5 transposase. This groundbreaking fusion seamlessly integrates the functionalities of transposase and Protein G, presenting a multifaceted solution tailored for the intricate demands of modern genomics.

Versatile Applications for CUT&Tag Technology

Unlock the potential of CUT&Tag technology, a cutting-edge approach for unraveling the intricacies of protein-genome interactions. pG-Tn5 Transposase's dual capabilities make it a powerhouse for applications in CUT&Tag, a revolutionary method that redefines protein-DNA interaction studies.

Advantages Over Traditional Methods

CUT&Tag stands out with a myriad of advantages over conventional ChIP-seq methods:

  • Time Efficiency: Streamlined experimental procedures for quicker results.
  • Low Cell Requirement: Minimized cellular input, optimizing resource utilization.
  • Low Background Signal: Achieves superior signal-to-noise ratios for robust data.
  • Good Reproducibility: Consistent and reliable results across experiments.
  • Single-Cell Sequencing Applicability: Pioneering capabilities for single-cell genomic exploration.

Unleashing the Power of Protein-Chromatin Interactions

CUT&Tag powered by pG-Tn5 Transposase emerges as a potent tool for unraveling protein-chromatin DNA interactions. This methodology holds immense promise in advancing our understanding of gene regulation and epigenetics.

Protein G's Dynamic Binding Capability

The Protein G component in pG-Tn5 Transposase exhibits remarkable versatility by primarily interacting with the Fc region of immunoglobulins. Its binding ability extends across IgG from a diverse array of mammalian species.

Elevate your genomic studies to unprecedented heights with pG-Tn5 Transposase – a catalyst for precision, efficiency, and innovation in the dynamic realm of genomics.