Protocols
- Large scale Int purification (3-100 liters)
- Small scale Int purification (0.25-3 liters)
- IHF purification protocol
- Xis purification protocol
- Western protocol PDF
- Int recombination and bandshift buffers PDF
- Making electrocompetent cells PDF
- Buffers PDF
- PFGE PDF
- MIC PDF
- Microscopy PDF
- TUNEL DNA Damage Detection Assay (updated) DOC
- 96 Well Template (updated) XLS
- Patching Grid (updated) PPT
- Small scale electroporation PDF
- Viability Experiment PDF
- Antibiotic Information (updated) DOC
- 6X Loading Dye for DNA DOC
- Alkaline lysis plasmid miniprep DOC
- B-Galactosidase assay - Microtiter plate format DOC
- Genomic DNA preparation (CTAB method) DOC
- Chemically-Competent Cell Recipe DOC
- EBU plates DOC
- Electrocompetent cells DOC
- Protocol for removing markers flanked by FRT sites DOC
- NCE Medium DOC
- P1 Transduction (E. coli) DOC
- DNase-free RNase DOC
- Sam's Red Swap Guide DOC
- Arbitrary PCR DOC
- 4X SDS-PAGE Sample Loading Buffer DOC
- Pfx50 Polymerase Formula XLS
- Taq Formula XLS