WSE-7160 EzStain AQua MEM: A Rapid and Reversible Protein Stain for Membrane Blotting


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WSE-7160 EzStain AQua MEM

WSE-7160 EzStain AQua MEM is a total protein stain kit for transfer membranes. It is a quick and easy-to-use kit that can be used to stain and destain proteins on PVDF and nitrocellulose membranes. The kit is also compatible with antibody reactions, so you can stain your membrane, destain it, and then use it for antibody binding.  

EzStain AQua MEM: A Rapid and Reversible Protein Stain for Membrane Blotting

Purpose and Application:

Versatile Staining and Destaining Solution Kit for Protein Detection

Our staining and destaining solution kit is designed for the precise detection of proteins on membranes post-blotting. The comprehensive features of this kit facilitate efficient antibody reactions by ensuring optimal blotting efficiency.

Key Applications:

  • Blotting Confirmation: Conduct staining prior to antibody reactions to validate the effectiveness of the blotting process.
  • Normalization Tool: Utilize results for normalizing target proteins after antibody reactions, enhancing accuracy in protein analysis.


  • Efficient Protein Detection:
    • The kit enables the reliable detection of proteins on membranes, providing crucial insights into the success of the blotting procedure.
  • Rapid Staining and Destaining:
    • Achieve quick and efficient staining and destaining processes, streamlining the overall workflow.
  • Distinctive Blue Band:
    • The solution produces a vivid and easily distinguishable bright blue band, ensuring clarity in visual analysis.
  • Post-Destaining Reactions:
    • Antigens and antibodies can seamlessly undergo reactions even after the destaining process, offering flexibility in experimental design.
  • Supports Total Protein Normalization:
    • Facilitates normalization of total proteins, contributing to accurate quantification and analysis.
  • Enhanced Sensitivity:
    • The kit exhibits higher sensitivity compared to traditional Ponceau S staining methods, enhancing the precision of protein detection.
  • Odor-Free Formulation:
    • Delivers a pleasant laboratory experience with no acetic acid odor, ensuring a comfortable working environment.

Elevate your protein detection processes with our staining and destaining solution kit, combining efficiency, accuracy, and user comfort for a seamless laboratory experience.


  • Membrane Staining Post-Blotting:After the completion of the blotting process, the transferred membrane underwent further analysis with two staining methods:
    • Conventional Staining (Ponceau S): One half of the membrane was subjected to staining using the traditional Ponceau S method.
    • WSE-7160 EzStain AQua MEM Staining and Destaining: The other half of the membrane underwent staining and subsequent destaining using the innovative WSE-7160 EzStain AQua MEM.
    The sample used for this analysis consisted of a 3/4 serial dilution of Hela cell extract. Notably, WSE-7160 EzStain AQua MEM successfully detected signals at all concentrations, showcasing its efficacy across a range of sample concentrations.

  • Antibody Reaction Following Staining:

Post-blotting, the membrane underwent a dual evaluation. One portion was subjected to a standard antibody reaction under normal conditions for chemiluminescence detection (A). Simultaneously, the other section of the membrane was treated with staining and destaining using WSE-7160 EzStain AQua MEM, followed by an antibody reaction for chemiluminescence detection (B). The results indicated that the antibody reaction remained unaffected by the staining process, demonstrating consistent sensitivity and patterns across both membranes.


WSE-7160 EzStain AQua MEM is a rapid and reversible protein stain for membrane blotting. It is a safe and environmentally friendly alternative to traditional stains such as Ponceau S and Coomassie Brilliant Blue (CBB). EzStain AQua MEM can be used to stain a variety of proteins, including large proteins (> 250 kDa). It is also compatible with a variety of membranes, including PVDF, nitrocellulose, and nylon. EzStain AQua MEM is an ideal choice for Western blotting, Northern blotting, and Southern blotting applications.

Unlocking Precision in DNA Staining with WSE-7160 EzStain AQua MEM

In the dynamic landscape of molecular biology, achieving precise and reliable DNA staining is paramount for accurate research outcomes. Introducing the WSE-7160 EzStain AQua MEM, a cutting-edge solution designed to redefine your DNA staining experience.

Key Features:

  1. Advanced Membrane Compatibility:
    • The WSE-7160 EzStain AQua MEM is engineered to excel in staining nucleic acids on various membranes, providing versatility in applications ranging from Southern and Northern blotting to Western blotting.
  2. Ultra-Sensitivity and High Resolution:
    • Benefit from exceptional sensitivity and resolution with the WSE-7160, ensuring that even the faintest DNA bands are vividly visualized. Capture the nuances of your experiments with unparalleled clarity.
  3. Rapid Staining Process:
    • Accelerate your workflow with the rapid staining capabilities of WSE-7160. Achieve brilliant results in a fraction of the time, enhancing efficiency without compromising on quality.
  4. Optimized for AQua MEM Gel Electrophoresis:
    • Tailored to complement AQua MEM gel electrophoresis systems, the WSE-7160 ensures seamless integration, facilitating a streamlined and efficient staining process.
  5. Safe and User-Friendly:
    • Prioritizing user safety, WSE-7160 eliminates the need for toxic ethidium bromide. The staining process is user-friendly, minimizing handling risks and promoting a safer laboratory environment.
  6. Quantitative and Reproducible Results:
    • Experience the reliability of quantitative results with WSE-7160. Achieve reproducibility in your experiments, empowering you with confidence in the accuracy of your findings.
  7. Wide Range of Applications:
    • Whether you are working with DNA fragments, RNA, or proteins, WSE-7160 adapts to diverse applications, providing a versatile solution for researchers across different fields of molecular biology.

Elevate Your DNA Staining Experience:

WSE-7160 EzStain AQua MEM is not just a staining solution; it's a catalyst for precision and efficiency in your laboratory endeavors. Embrace the future of DNA staining and unlock a new level of accuracy with the WSE-7160 EzStain AQua MEM.

Experience the Difference. Empower Your Research.

**WSE-7160 EzStain AQua MEM: A Paradigm-Shifting Solution for Protein Detection and Analysis

In the intricate realm of protein analysis, membrane blotting techniques have emerged as indispensable tools for researchers seeking to elucidate the intricate workings of biological systems. However, the traditional methods for protein visualization on membranes have often been hampered by time-consuming processes and the use of hazardous chemicals. WSE-7160 EzStain AQua MEM, a groundbreaking protein stain, revolutionizes the field of protein analysis by delivering a rapid, reversible, and user-friendly solution that seamlessly integrates with a wide range of research applications.

**Unveiling Protein Profiles with Unparalleled Efficiency

WSE-7160 EzStain AQua MEM stands as a testament to innovation, streamlining the protein detection process and delivering results with remarkable speed. The kit's innovative formulation facilitates rapid staining and destaining protocols, significantly reducing the overall time required for protein visualization. This enhanced efficiency proves invaluable in high-throughput experiments or when handling multiple samples, ensuring that researchers can rapidly gather the insights they need to drive their investigations forward.

**Unearthing Faint Protein Signals with Unparalleled Sensitivity

Beyond its rapid action, WSE-7160 EzStain AQua MEM distinguishes itself through its exceptional sensitivity, ensuring that even the faintest protein signals can be reliably detected. This enhanced sensitivity surpasses that of conventional staining methods, empowering researchers to detect proteins with greater precision and confidence. As a result, researchers can uncover subtle protein expression patterns and gain a deeper understanding of biological processes, leading to groundbreaking discoveries.

**Unleashing Flexibility and Versatility for Diverse Research Needs

WSE-7160 EzStain AQua MEM demonstrates remarkable versatility, seamlessly compatible with a wide range of membranes, including PVDF, nitrocellulose, and nylon. This adaptability extends to the detection of a diverse array of proteins, including large proteins (> 250 kDa), catering to a broad spectrum of research applications. Researchers can confidently utilize the kit across various experimental setups, assured of its ability to reliably detect a wide range of target proteins, regardless of their size or characteristics.

**Championing Sustainability and Environmental Stewardship

In line with the growing emphasis on sustainable laboratory practices, WSE-7160 EzStain AQua MEM stands as an environmentally friendly alternative to traditional stains such as Ponceau S and Coomassie Brilliant Blue (CBB). The kit's formulation eliminates the use of hazardous chemicals, minimizing environmental impact and promoting a safer working environment for researchers. This commitment to sustainability aligns with the growing movement towards eco-conscious laboratory practices and contributes to a healthier planet.

**Empowering Research Discovery and Advancement

WSE-7160 EzStain AQua MEM revolutionizes protein staining and destaining, offering a combination of speed, sensitivity, flexibility, and environmental friendliness that empowers researchers to achieve high-quality results with ease and confidence. Whether you're a seasoned scientist or a novice researcher, WSE-7160 EzStain AQua MEM is a powerful tool that will elevate your protein analysis endeavors, enabling you to unravel the intricate mysteries of protein biology and make groundbreaking discoveries.

**A Commitment to Scientific Excellence and Environmental Responsibility

Beyond its scientific contributions, WSE-7160 EzStain AQua MEM also promotes a safer and more sustainable laboratory environment, aligning with the growing focus on eco-conscious research practices. By minimizing the use of hazardous chemicals, the kit contributes to a healthier workplace and a more environmentally responsible approach to scientific research.

**Conclusion: A Transformative Force in Protein Analysis

In conclusion, WSE-7160 EzStain AQua MEM stands as a transformative tool in the arsenal of protein analysis, revolutionizing the way researchers visualize and analyze proteins. Its remarkable combination of speed, sensitivity, flexibility, and environmental friendliness makes it an indispensable resource for scientists across a wide range of disciplines. As researchers continue to push the boundaries of knowledge in molecular biology and beyond, WSE-7160 EzStain AQua MEM will undoubtedly remain at the forefront of protein analysis, enabling researchers to make groundbreaking discoveries that shape our understanding of the world around us.


WSE-7160 EzStain AQua MEM is a highly sensitive and easy-to-use protein stain kit for PVDF and nitrocellulose membranes. It provides a quick and convenient way to detect total protein levels, normalize protein levels, confirm protein transfer efficiency, visualize protein bands, and troubleshoot protein blotting experiments. The kit is compatible with antibody reactions, so you can stain your membrane, destain it, and then use it for antibody binding.

Overall, the WSE-7160 EzStain AQua MEM is an excellent choice for researchers who need a quick, sensitive, and easy-to-use protein stain kit for PVDF and nitrocellulose membranes.