GMP RNase Inhibitor: Protect Your RNA with the Highest Quality
GMP RNase Inhibitor is the highest quality RNase Inhibitor available, providing the ultimate protection for your RNA. With its high purity and specificity, GMP RNase Inhibitor can completely inhibit the activity of RNases, preventing RNA degradation
RNase Inhibitor (GMP-grade) - Advanced Protection Against RNase Activity
This product stands as a recombinant murine RNase inhibitor, meticulously expressed and purified in E. coli in its soluble form. It presents a formidable capacity to inhibit various RNase types, including A, B, and C, proving invaluable for applications involving RT-PCR and RT-qPCR. Distinguished from its human counterparts, this inhibitor lacks two cysteines highly prone to oxidation, ensuring heightened antioxidant efficacy. Manufactured in adherence to GMP process requirements, this inhibitor is conveniently supplied in liquid form.
Key Attributes:
- Potent Antioxidant Capacity: Bolstered defense against oxidative influences.
- Effective Inhibition: Demonstrates superior inhibition effects with heightened overall activity.
- GMP-Compliant Production: Conforms to stringent GMP standards, ensuring quality and reliability.
Versatile Applications:
- RNase Inactivation: Safeguard your samples against RNase degradation.
- PCR and qPCR: Enhance the precision and reliability of your PCR and qPCR experiments.
RNase Inhibitor (GMP-grade) - Superior Quality in a Liquid Formulation
| Product Name | RNase Inhibitor (GMP grade) |
|---|---|
| Source | Recombinant expression in Escherichia coli |
| Catalog Number | CSB-DEM080 |
| Physical Form | Liquid |
| Enzyme Activity | 40 U/L |
| Storage Conditions | -20±5°C |
| Molecular Weight | 50 kDa |
| Storage Buffer | 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol, pH 7.6 |
| Activity Definition | One unit (U) is defined as the amount of enzyme that inhibits 50% of the activity of 5 ng RNase A. |
| Quality Control | Free from endonucleases, exonucleases, and RNase contamination |
| Shelf Life | 24 months |
RNase Inhibitor - Unrivaled RNase Protection
Efficiently and non-covalently inhibiting the activity of RNase A, B, and C in a 1:1 ratio, our RNase Inhibitor boasts a binding constant exceeding 10^14. This exceptional product leaves RNase 1, RNase T1, S1 nuclease, RNaseH, and RNase derived from Aspergillus species unaffected. Furthermore, when paired with polymerases like Taq DNA polymerase, AMV or M-MuLV reverse transcriptase, and bacteriophage RNA polymerases (SP6, T7, or T3), Mammalian RNase Inhibitor does not compromise their activity.
Product Components
| Label | Components | Specifications |
|---|---|---|
| 1 | RNase Inhibitor | 4000 U, 20000 U, 40000 U |
Elevate your experiments with the unmatched RNase protection provided by our premium RNase Inhibitor.
Operational Guidelines
Utilize this inhibitor in cDNA first-strand synthesis, RT-PCR, and RT-qPCR systems to ensure optimal results. Safeguard RNA integrity during in vitro transcription/translation processes. Employ it to inhibit RNase activity effectively during RNA isolation and purification procedures. Note that the RNase Inhibitor retains its inhibitory activity within the pH range of 5-8, exhibiting maximum inhibition at pH 7-8. Additionally, it is recommended to use the RNase Inhibitor at temperatures below 50°C for reliable outcomes.
GMP RNase Inhibitor: A Pure and Reliable Tool for RNA Protection
GMP RNase Inhibitor is a highly purified enzyme that potently inhibits the activity of RNases, a class of enzymes that degrade RNA. It is manufactured under strict Good Manufacturing Practices (GMP) guidelines, ensuring the highest quality and purity. GMP RNase Inhibitor is an essential tool for a wide range of research and clinical applications, including:
- mRNA vaccine production: GMP RNase Inhibitor is used to protect the mRNA during production and storage, ensuring its integrity and efficacy.
- In vitro transcription: GMP RNase Inhibitor is used to protect the RNA transcripts during in vitro transcription reactions, guaranteeing their high quality and utility for downstream applications.
- RNA probe preparation: GMP RNase Inhibitor is used to protect RNA probes during preparation, safeguarding their integrity and ensuring their accurate performance in target detection and quantification assays.
- RNA sequencing: GMP RNase Inhibitor is used to protect RNA samples during RNA sequencing library preparation and sequencing, guaranteeing the accuracy and reliability of the data obtained.
- Proteomics: GMP RNase Inhibitor is used to protect RNA during proteomics workflows, such as ribosome profiling and RNA-protein interaction studies, enabling accurate and reliable results.
GMP RNase Inhibitor is easy to use and can be added to RNA samples at any time to protect them from degradation. It is also compatible with a wide range of buffers and reagents, making it a versatile tool for your research needs.
Additional Benefits of GMP RNase Inhibitor:
- High efficiency and specificity: GMP RNase Inhibitor completely inhibits the activity of RNases, even in the presence of high levels of RNases.
- Broad range of activity: GMP RNase Inhibitor is effective against a wide range of RNases, including RNase A, RNase H, and RNase I.
- High stability: GMP RNase Inhibitor is stable at a wide range of temperatures and pH values.
- Low toxicity: GMP RNase Inhibitor is non-toxic and can be used safely in a variety of applications.
GMP RNase Inhibitor works by binding to RNases and preventing them from interacting with RNA. This prevents the RNases from cleaving the RNA molecules, thereby protecting them from degradation.
GMP RNase Inhibitor is a highly specific enzyme, meaning that it only binds to RNases and does not bind to other proteins or nucleic acids. This makes it a safe and effective tool for protecting RNA in a variety of applications.
GMP RNase Inhibitor is also very efficient at inhibiting the activity of RNases. Even in the presence of high levels of RNases, GMP RNase Inhibitor can completely inhibit their activity. This makes it a very reliable tool for protecting RNA from degradation.
How GMP RNase Inhibitor works:
- GMP RNase Inhibitor binds to the active site of the RNase enzyme.
- Once bound, GMP RNase Inhibitor blocks the RNase from binding to RNA.
- This prevents the RNase from cleaving the RNA molecules.
- As a result, the RNA molecules are protected from degradation.
GMP RNase Inhibitor is a valuable tool for a wide range of research and clinical applications. It is essential for protecting RNA during mRNA vaccine production, in vitro transcription, RNA probe preparation, RNA sequencing, and proteomics workflows. GMP RNase Inhibitor is also used to protect RNA in other applications, such as forensic science and diagnostic testing.