mRNA Cap 2'-O-Methyltransferase (GMP-grade): The Purest and Most Reliable CapMTase for Your RNA Research
mRNA Cap 2'-O-Methyltransferase (GMP-grade) is the purest and most reliable CapMTase available, making it the ideal choice for a wide range of RNA research applications. With its high efficiency and specificity, mRNA Cap 2'-O-Methyltransferase (GMP-grade) can completely methylate the 2'-O position of the first nucleotide of the Cap0 structure at the 5' end of RNA in minutes, without damaging other biomolecules.
mRNA Cap 2'-O-Methyltransferase (GMP-grade): The Purest and Most Reliable CapMTase for Your RNA Research
mRNA Cap 2'-O-Methyltransferase (GMP-grade) is the purest and most reliable CapMTase available, making it the ideal choice for a wide range of RNA research applications. It catalyzes the methylation of the 2'-O position of the first nucleotide of the Cap0 structure at the 5' end of RNA, which is essential for RNA stability, translation, and splicing.
GMP mRNA Cap 2'-O-Methyltransferase is produced under the strictest Good Manufacturing Practices (GMP) guidelines, ensuring the highest quality and purity. It is also highly efficient and specific, and can completely methylate RNA transcripts in minutes, without damaging other biomolecules.
GMP mRNA Cap 2'-O-Methyltransferase is widely used in a variety of RNA research applications, including:
- mRNA vaccine production: mRNA Cap 2'-O-Methyltransferase is used to cap mRNA transcripts during the production of mRNA vaccines. This ensures that the mRNA transcripts are stable and can be efficiently translated into proteins by the body.
- In vitro transcription: GMP mRNA Cap 2'-O-Methyltransferase is used to cap RNA transcripts during in vitro transcription reactions. This ensures that the RNA transcripts are stable and can be used for downstream applications, such as RNA sequencing and protein expression.
- RNA probe preparation: GMP mRNA Cap 2'-O-Methyltransferase is used to cap RNA probes during RNA probe preparation. This ensures that the RNA probes are stable and can be used accurately to detect and quantify specific RNA targets.
- RNA sequencing: GMP mRNA Cap 2'-O-Methyltransferase is used to cap RNA transcripts during RNA sequencing library preparation. This ensures that the RNA transcripts are stable and can be efficiently sequenced.
- Proteomics: GMP mRNA Cap 2'-O-Methyltransferase is used to cap RNA transcripts during proteomics workflows, such as ribosome profiling and RNA-protein interaction studies. This ensures that the RNA transcripts are stable and can be accurately analyzed.
If you are working with RNA in your research, GMP mRNA Cap 2'-O-Methyltransferase is an essential tool for ensuring the stability, quality, and reliability of your results.
Order your GMP mRNA Cap 2'-O-Methyltransferase today and experience the difference!
Advanced mRNA Capping with Precision: mRNA Cap 2´-O-Methyltransferase (GMP-grade)
Welcome to the pinnacle of mRNA modification! Our mRNA Cap 2´-O-Methyltransferase, a stellar recombinant protein sourced from the vaccinia virus, stands as a groundbreaking enzyme in the world of molecular biology. This exceptional catalyst orchestrates the addition of a methyl group to the 2´-O position of the initial nucleotide adjacent to the cap structure at the 5´ end of RNA.
Harnessing SAM (S-adenosylmethionine) as its methyl donor, this enzyme skillfully methylates capped RNA, crafting the sophisticated Cap1 structure. The Cap1 structure emerges as a linchpin, elevating the translation efficiency of mRNA. In practical terms, this translates into an augmentation of expression in mRNA transfection and microinjection experiments.
Distinctive Features:
- Exemplary performance with complex structures
- High efficiency in methyl group addition
- Robust stability in diverse experimental conditions
GMP-Certified Excellence: Embrace the confidence that comes with a GMP-compliant production system, ensuring not just quality but a paradigm of excellence.
Applications Beyond Boundaries: Engage in the art of RNA capping methylation, unlocking new dimensions in your molecular explorations.
Elevate your research with the precision of mRNA Cap 2´-O-Methyltransferase – where innovation meets reliability.
Product Name | mRNA Cap 2´-O-Methyltransferase (GMP-grade) |
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Source | Recombinant expression in Escherichia coli |
Catalog Number | CSB-DEM083 |
Physical Form | Liquid |
Enzyme activity | 50 U/L |
Storage Conditions | -20±5°C |
Storage Buffer | 20 mM Tris-HCl pH 8.0, 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 50%(v/v) glycerol, 0.1% (v/v) Triton X-100 |
Activity definition | The enzyme amount required to methylate 10 pmol of 80 nt capped RNA transcript within 1 hour at 37°C is defined as one unit (U) |
Shelf Life | 12 months |
Product Description:
mRNA Cap 2´-O-Methyltransferase, a recombinant protein derived from the cowpox virus, exhibits the capability to add a methyl group specifically to the 2´-O position of the initial nucleotide adjacent to the cap structure at the 5´ end of RNA. Employing SAM as a methyl donor, this enzyme methylates capped RNA, generating the Cap1 structure. The Cap1 structure significantly enhances mRNA translation efficiency, proving advantageous in mRNA transfection and microinjection experiments. Notably, the enzyme requires RNA with an m7GpppN cap structure as a substrate.
Product Components:
Product Components | Specifications |
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mRNA Cap 2´-O-Methyltransferase (GMP-grade) | 10KU, 50KU, 250KU |
10×Capping Reaction Buffer | 0.4mL, 2mL, 10mL |