"Gentaur Molecular" operates five major technical platforms concurrently, supporting a cutting-edge special enzyme research and development as well as production system. We offer:

  • A complete range of mRNA raw materials and various mRNA stock solutions, continually optimizing and enhancing our products, with key quality indicators of synthetic products at the forefront.
  • End-to-end CRO services, encompassing template production, in vitro transcription, capping and tailing, LNP encapsulation, and quality control.
  • Comprehensive and top-notch mRNA quality inspection platforms, supported by methods such as capillary electrophoresis, high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and more."

 mRNA BsaI 


Bsa I , an IIs restriction endonuclease restriction endonuclease, is derived from a recombinant E.coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus. which have the same specificity as native enzymes and reduced star activity. 

Its recognition sequence and cleavage sites are as follows:





Catalog Size
HBP001306 1KU
HBP001307 5KU
HBP001308 200KU
HBP001309 2000KU

Product component

BsaI(20 U/μL)50 μL250 μL
10 x BsaI Buffer1.25 mL1.25 mL
BsaI (20 U/μL)10 mL100 mL
10 x BsaI Buffer5×10 mL5×100 mL


Store at -20 ℃, valid for 1 year( Avoid repeated freeze-thaw cycles)

Storage buffer

10 mM Tris-HCl, 200 mM NaCl, 1 mM DTT 0.1 mM EDTA, 200 µg/ml Recombinant Albumin,50% Glycerol. (pH 7.4 @ 25°C).

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of Internal control DNA in 1 hour at 37°C in a total reaction volume of 50 µL.

Quality Control

Protein Purity Assay (SDS-PAGE): The purity of Bsa I was ≥ 95% determined by SDS-PAGE analysis using Coomassie Blue detection.

RNase: 20 U of Bsa I with 1.6 μg MS2 RNA for 16 hours at 37 ℃ yields no degradation as determined by agarose gel electrophoresis.

Non-Specific DNase Activity: 20 U of Bsa I with 1 μg PhiX174 DNA for 16 hours at 37 ℃ yields no excess DNA as determined by agarose gel electrophoresis.

Star activity: 40 U of Bsa I with 1 μg λ DNA/HindIII for 16 hours at 37 ℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.

Ligation and Recutting: After digestion of 1 μg λDNA/HindIII with 20 U BsaI, DNA fragments can be ligated with T4 DNA ligase at 16ºC. And these ligated fragments can be recut with BsaI.  

E.coli DNA: 2 U of Vaccinia virus Capping Enzyme is screened for the presence of E. coli genomic DNA using TaqMan qPCR with primers specific for the E. coli 16S rRNA locus. The E. coli genomic DNA contamination is≤1 E.coli genome.

Bacterial Endotoxin: LAL-test, according to Chinese Pharmacopoeia IV 2020 edition, gel limit test method, the general rule (1143). Bacterial endotoxin content should be ≤10 EU/mg.

Reaction system and conditions

BsaI(20 U/μL)1 μL
DNA1 μg
10 x BsaI Buffer5 μL
dd H2OUp to 50 μL

Incubate at 37°C for 15-30 minutes Heat Inactivation:80°C for 20 min

Product features

1.High activity 

2. Fast digestion

3.Low star activity , ensuring accurate cutting like “scalpel”; Without BSA and animal-origin free.

Methylation Sensitivity

dam methylation: Not Sensitive;

dcm methylation: Impaired by Some Combinations of Overlapping;

CpG Methylation: Blocked by Some Combinations of Overlapping.

Notes on use

  1. The volume of enzyme ≤ 1 / 10 of the reaction volume.
  2. Star activity may occur when glycerol concentration is more than 5%.
  3. Cleavage activity may occur when Substrate below the recommended ratio.



 BspQI can be recombinantly expressed in E. coli that can recognize specific sites and are produced under GMP standards. It is widely used in gene mapping, cloning and other fields. This enzyme rapidly cuts DNA for efficient gene linearization. 


Cat. No. Size
HBP001401 0.5 KU
HBP001402 2.5 KU
HBP001403 100 KU
HBP001404 1000 KU

Product Component

BspQI (10 U/μL)50 μL250 μL
10 x Buffer1.25 mL1.25 mL
BspQI (10 U/μL)10 mL100 mL
10 x Buffer12.5 mL125 mL

Shipping and Storage conditions

The product should be shipped ≤ 0℃; Stored at -20℃ condition.

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 µl.

Recognition Site

Quality Control

Purity≥95%, no DNase, RNase activity, Host DNA residue≤100pg/mg, Host protein residue ≤50ppm, Endotoxin residue≤10EU/mg, No protease activity, sterile, no mycoplasma.

Reaction system and conditions

BspQI(10 U/μL)1 μL
DNA1 μg
10 × Buffer5 μL
dd H2OUp to 50 μL

Reaction time:50℃15~30 min。

Product Features

  • The restriction endonuclease has high enzymatic activity and completes the digestion quickly.
  • Low non-specific endonuclease activity to ensure precise “scalpel” cutting.
  • No BSA system, reducing heat source pollution.


  • RNA Vaccine Preparation
  • Restriction Enzyme Digestion
  • Fast Cloning.


Separate email addresses with a comma.