Lymphoprep is a ready-made, sterile and endotoxin
tested solution with the following specifications:

• Sodium diatrizoate 9.1% (w/v)
  Polysucrose 400 5.7% (w/v)
  Density 1.077 ± 0.001 g/ml
  Osmolality 290 ± 15mOsm
  Endotoxins < 1.0
  Iodixanol
  Mw = 1550 g/mol

Optiprep

                                                                                                                                                                                                   

                                                                                                                                                                                                              OptiPrep™

                                                                                                                                                                                                                                   AXS-1114542

OptiPrep is a sterile and endotoxin tested solution of 60% iodixanol in water with a density of 1.320 g/ml. Iodixanol was
developed as an X-ray contrast medium an has therefore been subjected to rigorous clinical testing. It is non-ionic, non-toxic
to cells and metabolically inert. Iodixanol solutions can be made isoosmotic at all useful densities, this solutions have low
viscosity and osmolality.

The high density of OptiPrep facilitates the fractionation of cells by flotation from a dense load zone through either
a continuous or discontinuous gradient or through a simple density barrier.
OptiPrep
The optimum density gradient medium

Applications

• Viruses
• Plasma lipoproteins
• Proteins and protein complexes
• Plasmid DNA
• Ribonucleoproteins
• Mammalian and non-mammalian cells
• Subcellular organelles
• Plasma membranes and domains
• Membrane vesicles and cytosol
• Organelles from non-mammalian sources
Isolation of:
• Membrane trafficking and cell signalling
Analysis of:
• Endocytosis and exocytosis

Lymphoprep Tube

Lymphoprep™ Tube

    

AXS-1019818/AXS-1019817

1. Separation of leucocytes from blood and bone marrow by
   Lymphoprep Tube
   
   

2. Isolation of human mononuclear cells – easy handling
   
   

3. Each batch of Lymphoprep is checked on the level of
   endotoxins using a specific LAL test. 

4. Our goal is to produce
   batches with an endotoxin level lower or equal to
   0.13 IU/ml.

5. For every batch produced a Certificate of Analysis showing
   the actual values of density, osmolality and endotoxins are measured.

 

Polymorp ​ hprep

Behaviour of Erythrocytes during centrifugation with Polymorphprep

With the exception of the basophils, polymorphonuclear leukocytes (PMNs) have a much greater buoyant density than the
mononuclear cells, >1.085 g/ml. Unfortunately, the buoyant density of the erythrocytes tends to be from 1.09 – 1.11 g/ml,
this makes a separation from whole blood using a density barrier similar to that used for mononuclear cells more difficult.
A number of procedures have been developed in an effort to overcome these difficulties.
The high osmolality of Polymorphprep causes erythrocytes to lose water and shrink, thus increasing their effective
buoyant densities. This allows the aggregated erythrocytes to sediment rapidly through the dense medium.
Each batch of Polymorphprep is checked on the level of endotoxins using a specific LAL test. Our goal is to produce
batches with an endotoxin level lower or equal to 0.13 IU/ml.
The method is effective only with whole undiluted human blood not with a leukocyterich fraction or blood from
animal species.
The temperature is important to obtain optimal results, as changes in temperature effect the density and viscosity of the
Polymorphprep solution. The temperature of the blood sample and the medium should be kept between 18 - 22 °C.
Analysis of the top and bottom bands from the Polymorphprep separation using a Coulter STKR Cell Analyser is shown in
the figure on the right. The analyzer determines the number of cells in the sample (ordinate) as a function of cell volume
(abscissa). Relative cell number is the number of cells of a particular volume expressed as a fraction of the total in each
sample.

Polymorphprep
Isolation of human polymorphonuclear cells
Polymorphprep is supplied as
a sterile solution in the following package sizes:
Prod. No. 1895 1 x 250ml

 

Nycodenz

Nycodenz is an off-white powder, freely soluble in water. 

                                                                                                                                                                                                                                         

Nycodenz AXS-1002424

1. Solution up to 80% (w/v) with a density of 1.426 g/ml can be prepared.

2. It was originally developed as an X-ray contrast medium and have therefore been subjected to rigorous clinical testing.

• non-ionic, non-toxic to cells and metabolically inert
• can be used for the isolation of cells, subcellular organelles and membranes, macromolecules and viruses
• forms true solutions. It is therefore easy to remove the medium from the cells after fractionation
• is resistant to bacterial degradation

• solutions can be autoclaved

 

 Gradients of Nycodenz can be generated by:

• centrifugation in situ (self-forming gradients).
• diffusion. Using Nycodenz, linear gradients can be simply prepared within 45 minutes.
• freezing and thawing.
• tilted tube rotation (Gradient Master™).

The density of Nycodenz in solution can be determined by
measuring the refractive index. The density can also be
determined spectrophotometrically.
Nycodenz is a non-particulate medium idem to Gentodenz therefore the
distribution of cells in gradients can be determined using a
haemocytometer, electronic particle counter or by light
scattering measurements using a spectrophotometer.
Nycodenz does not interfere with the orcinol and
diphenylamine reactions for the estimation of nucleic
acids nor with the very sensitive dye binding assays for
protein and DNA.

Polysaccharides and sugars can be determined in the presence of Nycodenz using the phenol/H2
SO4 assay. Fluorimetric assays of nucleic acids and proteins can also be carried out in the presence of Nycodenz. Nycodenz does not interfere
with most assays for the marker enzymes of subcellular components, also commercial scintillants are compatible with Nycodenz.
Nycodenz can be removed from samples by dialysis, ultrafiltration or gel filtration. Cells, subcellular organelles and
other particulate matter can be isolated from Nycodenz by centrifugation without the risk of contaminating the pellet
with Nycodenz.

Nycodenz
A universal density gradient medium
Nycodenz is supplied as
a powder in the following package size:
Prod. No. 18003 1 x 500 g

Applications

Isolation of:
• Mammalian and non-mammalian cells
• Subcellular organelles
• Organelles from non-mammalian sources
• Subcellular membranes
• Protein and protein complexes
• Ribonucleoproteins
• Viruses

Polysucrose

Polysucrose 400 is a synthetic high molecular weight polymer made by the copolymerization of sucrose and
epichlorohydrin. The molecules have a branched structure with a high content of hydroxyl groups giving a good solubility in
aqueous solutions.
The reactivity and stability of Polysucrose 400 are determined by its hydroxyl groups and the glycosidic bonds in the
sucrose residues. 

Polysucrose 400 is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly
hydrolysed, especially at elevated temperatures. In neutral solutions, Polysucrose 400 can be sterilized by autoclaving at
110 °C for 30 minutes without any degradation.
Polysucrose 400 is readily soluble in aqueous solutions when added slowly to the liquid with constant stirring.
Concentrations up to 50% (w/v) can easily be obtained.

• Polysucrose 400 A universal density gradient medium
• Prod. No. 7828 20 kg

Technical data

White odourless powder
Absorption <0.45
Specific optical rotation (α)
d 53° – 59°
Intrinsic viscosity (20 °C) 0.14 – 0.20
Average molecular weight (Mw) 450,000 ± 100,000 g/mol
Mw distribution by GPC conforms to standard
Loss on drying (%) <5.0%
pH (10% w/v aqueous solution) 7.0 – 9.0
Sulphated ash <0.3%
Content of chloride (ppm) <500 ppm
Sterilization test conforms
Microbiological contamination
<100CFU/g
<10 yeasts and mould/g
Bacterial endotoxins <10.0 IU/g