Molecular Kits: Unleashing the Power of Genetic Analysis
Explore the vast world of molecular biology with our comprehensive range of Molecular Kits, designed to empower researchers and clinicians with cutting-edge tools for genetic analysis, diagnostics, and research.
Product Name: One Step RT-qPCR Kit
Physical Form: Liquid
Storage Conditions: -20 ±5°C, avoid repeated freeze-thaw cycles; can be stored at 2-8°C for frequent use
Transportation Conditions: ≤0°C; transport on dry ice
Product Components: Buffer system, dNTPs, Taq DNA Polymerase, UDG, M-MLV reverse transcriptase, RNase Inhibitor
Quality Control: All components have been tested and found to be free of nucleases, endonucleases, and residual RNases.
Shelf Life: 12 months
The One Step RT-qPCR Kit is a comprehensive reagent kit designed for quantitative PCR reactions with RNA as a template. In the experimental process, reverse transcription and quantitative PCR are seamlessly integrated into a single reaction tube, streamlining the experimental procedure. The kit leverages the capabilities of heat-resistant M-MLV reverse transcriptase to efficiently synthesize the first strand cDNA. It also incorporates HS Taq DNA Polymerase for robust and quantitative amplification. Specifically tailored for systems utilizing fluorescent probes, this kit facilitates one-step amplification, making it a powerful tool for precise and sensitive detection.
|50T||2×One-step buffer Mix||0.75mL, 1.5mL, 7.5mL|
|100T||One Step RT-qPCR Taq Mix||50μL, 100μL, 500μL|
SYBR PCR Mix (2x SYBR Green qPCR MiX) is a pre-mixed, 2x concentrated master mix for real-time PCR (qPCR) using SYBR Green I dye for detection of double-stranded DNA (dsDNA). It contains all the necessary components for qPCR, including:
- Taq DNA Polymerase
- dNTPs (including dUTP)
- SYBR Green I dye
- Optimized PCR buffer
- High sensitivity and specificity
- Broad detection range
- Convenient 2x pre-mixed format
- Room temperature stability
- Compatible with a wide range of real-time PCR instruments
- Gene expression analysis
- cDNA synthesis
- miRNA detection
- SNP genotyping
- Pathogen detection
- Forensic analysis
Product Storage and Stability
Store SYBR PCR Mix (2x SYBR Green qPCR MiX) at -20°C. Upon thawing, the mix is stable for up to 30 days at 4°C or up to 1 year at -20°C.
- Sensitivity: 10 copies of target DNA
- Detection range: 20 log copies of target DNA
- Linearity: 4-5 logs
- Compatibility: A wide range of real-time PCR instruments
- SYBR PCR Mix (2x SYBR Green qPCR MiX) is a high-performance, easy-to-use qPCR master mix that delivers sensitive and specific detection of dsDNA.
- The pre-mixed format saves time and reduces pipetting errors.
- The mix is room temperature stable, making it convenient for everyday use.
- The mix is compatible with a wide range of real-time PCR instruments, making it a versatile tool for a variety of applications.
Product Ordering Information
SYBR PCR Mix (2x SYBR Green qPCR MiX) is available in a variety of sizes to meet your needs. Please contact your local distributor for more information.
Overall, SYBR PCR Mix (2x SYBR Green qPCR MiX) is a valuable tool for researchers who need a reliable and easy-to-use qPCR master mix for detection of dsDNA.
2×Taq PCR premix is a ready-to-use, optimized solution for conventional PCR amplification reactions. It streamlines the PCR workflow by eliminating the need for individual component preparation, minimizing the risk of contamination during PCR setup. The premix comes in two variants: regular and fast-loading. Extensive testing has demonstrated that the presence of tracking dyes does not interfere with PCR performance, allowing direct visualization of amplified DNA fragments on agarose gels following PCR completion, saving valuable time.
- Simplified Operation: Requires only the addition of template DNA and primers for PCR amplification.
- Enhanced Reproducibility: Reduces the number of manual pipetting steps, minimizing contamination and experimental errors.
- Time-Saving Efficiency: Contains electrophoresis dyes, enabling direct gel electrophoresis after PCR completion, simplifying the overall workflow.
- Broad Applicability: Optimized components ensure compatibility with a wide range of conventional PCR reactions.
- Conventional PCR amplification/identification
- TA cloning
|Dye||Blue and yellow tracking dyes for accurate and convenient gel electrophoresis|
|Compatibility||Compatible with a wide range of conventional PCR instruments|
|Storage||Store at -20°C|
|Shelf life||Stable for up to one year at -20°C|
Gel Electrophoresis Detection
Note: 1-3, 4-6, 7-9, 10-12, 13-15 are respectively: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5. Line1, 4, 7, 10, 13: 2×Taq PCR Mix (with dye).
Line2, 5, 8, 11, 14: 2×Taq PCR Mix (without dye, add dye after PCR).
Line3, 6, 9, 12, 15: 2×Taq PCR Mix (without dye, add regular loading buffer after PCR).
M: Trans2K DNA Marker.
Note: 1-4, 5-8, 9-12, 13-16, 17-20: Primer 1, Primer 2, Primer 3, Primer 4, Primer 5
Line1, 5, 9, 13, 17: Placed at 37°C for 7 days
Line2, 6, 10, 14, 18: Placed at 37°C for 14 days
Line3, 7, 11, 15, 19: Frozen at -20°C
Line4, 8, 12, 16, 20: 2×Taq PCR Mix (no dye) + dye (after PCR completion)
M: Trans2K DNA Marker
Comparison test with competitor’s kit
Line1-4：CSB-DKT033 M：Trans2K DNA Marker
Overall, 2×Taq PCR premix offers a convenient, reliable, and efficient solution for conventional PCR amplification, making it an essential tool for molecular biology research.
Seamless Cloning Kit: A Versatile Tool for Efficient and Precise DNA Assembly
The Seamless Cloning Kit provides a powerful and straightforward approach for directional cloning of DNA fragments into a linearized vector. This kit eliminates the need for restriction enzyme digestion, streamlining the cloning process and enhancing efficiency.
- Flexibility and Specificity: Enables gene cloning at any desired position within the target vector, ensuring precise insertion of DNA fragments.
- Rapid and Convenient: The seamless cloning process can be completed within a single hour, minimizing experimental time and effort.
- High Cloning Efficiency: Achieve positive cloning rates of up to 90% or higher, ensuring successful gene transfer and expression.
- Multiplex Cloning Capability: Simultaneously recombine multiple target genes into a single vector, facilitating efficient multigene cloning projects.
- Rapid Cloning: Streamline the construction of genetic constructs for functional studies, protein expression, and gene therapy applications.
- High-Throughput Cloning: Efficiently create large libraries of genetic variants for high-throughput screening and functional genomics studies.
- Seamless Cloning: Achieve seamless integration of DNA fragments without introducing unwanted scar sequences, preserving the integrity of the cloned genes.
- DNA Site-Directed Mutagenesis: Introduce precise mutations into genes of interest for functional analysis and protein engineering.
Seamless Cloning Kit: Your Gateway to Efficient and Precise Gene Assembly
With its simplicity, efficiency, and versatility, the Seamless Cloning Kit has become an invaluable tool for molecular biologists, enabling them to seamlessly integrate DNA fragments into vectors for a wide range of research applications.
Blood Direct PCR Kit: A Robust Tool for Direct PCR Amplification from Whole Blood
The Blood Direct PCR Kit introduces a novel anti-inhibitory DNA polymerase, a next-generation hot start Taq DNA polymerase engineered using antibody modification technology. This enhanced Taq DNA polymerase maintains its exceptional performance even in the presence of commonly used anticoagulants, exhibiting remarkable resistance to a range of potent PCR inhibitors.
Leveraging the capabilities of this innovative DNA polymerase, the Blood Direct PCR Kit enables direct PCR amplification from whole blood samples without the need for prior DNA purification or sample pretreatment. It can amplify DNA fragments from whole blood concentrations of up to 30%, offering a convenient and efficient solution for PCR analysis of blood samples.
- Maintains Activity in the Presence of Anticoagulants: The modified Taq DNA polymerase effectively functions in the presence of various anticoagulants, including heparin, sodium citrate, and EDTA, ensuring compatibility with a wide range of blood sample preparation protocols.
- Amplification of Blood Extraction Samples and Blood Lysis Crude Products: The kit is capable of amplifying DNA from both blood extraction samples and blood lysis crude products, providing flexibility in sample preparation and analysis.
- High Tolerance to Impurities: The Blood Direct PCR Kit exhibits exceptional tolerance to impurities, making it suitable for a variety of detection scenarios, including those involving contaminated or challenging blood samples.
- PCR Amplification from Whole Blood: The kit is specifically designed for direct PCR amplification from whole blood samples, eliminating the need for DNA purification and simplifying the overall workflow.
- Genomic Fragment Amplification: The Blood Direct PCR Kit can effectively amplify genomic fragments of up to 6kb from whole blood, making it suitable for a wide range of genetic studies and diagnostic applications.
Line 1, 2: Brand Y blood-resistant enzyme
Line 3, 4: Brand S blood-resistant enzyme
Line 5、6：Hemo Taq DNA Polymerase
Blood direct amplification fragment length