Pinnacle of Genomic Precision: Unveiling pAG-Tn5 Transposase
Innovative Fusion Redefining Genomic Research
Introducing pAG-Tn5 Transposase, a groundbreaking fusion of Protein A/Protein G with a potent, mutant form of Tn5 transposase. This unique product seamlessly integrates the dual functionalities of Tn5 transposase and Protein A/Protein G, setting new standards in genomics. The incorporated mutant Tn5 transposase boasts an unprecedented 1000-fold increase in in vitro transposition efficiency compared to the wild type.
Key Features Setting the Benchmark
- Dual Functionality: Proficiently encompasses both the functions of Tn5 transposase and Protein A/Protein G.
- Enhanced Efficiency: Showcases an extraordinary 1000 times higher in vitro transposition efficiency than the wild type.
Tailored for CUT&Tag Excellence in Research
This product presents a dynamic duo of pAG-Tn5 transposase and its associated assembly buffer, meticulously crafted for precision in CUT&Tag technology. Ideal for protein-genome interaction studies, this fusion promises unparalleled efficiency in your genomic explorations.
Versatile Applications for Cutting-Edge Research
Product Applications:
- Second-generation Sequencing Library Construction
- ATAC-seq (Assay for Transposase-Accessible Chromatin using Sequencing)
- CUT&Tag (Cleavage Under Targets & Tagmentation)
Why Choose pAG-Tn5 Transposase?
In a league of its own, pAG-Tn5 Transposase offers a transformative approach to genomic studies. From dual functionality to specific suitability for CUT&Tag technology, it redefines the landscape of protein-genome interaction research.
Elevate your research to unparalleled heights with pAG-Tn5 Transposase – where innovation meets precision in the dynamic realm of genomics.
| Property | Value |
|---|---|
| Product Name | pAG-Tn5 Transposase for CUT&Tag |
| Source | Recombinant expression in Escherichia coli |
| Catalog Number | CSB-DEM070 |
| Physical Form | Liquid |
| Storage Conditions | -20°C |
| Molecular Weight | 75 kDa |
| Storage Buffer | 50mM HEPES (pH 7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol |
| Quality Control | No residual nucleases and exonucleases |
| Shelf Life | 12 months |
Product Overview
Introducing our cutting-edge pAG-Tn5 Transposase, an innovative fusion enzyme seamlessly merging Protein A/G with a modified, high-activity Tn5 transposase. This unique combination yields a novel enzyme with dual functionality, possessing both transposase and Protein A/G activities. Tailored for the revolutionary CUT&Tag technology, specifically designed for protein-genome interaction studies, this fusion enzyme unlocks unparalleled potential in your research endeavors.
CUT&Tag stands as a beacon of advancement in the realm of protein-DNA interaction studies, offering a myriad of advantages over traditional ChIP-seq methods. Embracing features such as time efficiency, minimal cell requirements, low background signal, high reproducibility, and applicability in single-cell sequencing, CUT&Tag emerges as a powerful and versatile tool. Its application extends to the intricate exploration of protein-chromatin DNA interactions, providing profound insights into gene regulation and epigenetics.
The Protein A/G fusion within this exceptional product exhibits a predominant interaction with the Fc region of immunoglobulins, facilitating binding to IgG from a diverse array of mammals. Elevate your research capabilities with pAG-Tn5 Transposase, where innovation converges with precision for groundbreaking discoveries in the field of molecular biology.
| Label | Components | Specifications |
|---|---|---|
| 1 | pAG-Tn5 Transposase (2 mg/mL) | 10µL |
| 2 | Annealing Buffer | 500µL |
| 3 | Tagment Buffer (5X) | 300µL |
| 4 | Stop Buffer (5X) | 300µL |
pAG-Tn5 Transposase for CUT&Tag is a unique fusion protein that combines the DNA transposition activity of Tn5 transposase with the protein A and protein G domains. This fusion protein enables the targeted integration of DNA adapters into genomic DNA at specific sites defined by antibodies, a process known as Cleavage Under Targets and Tagmentation (CUT&Tag).
CUT&Tag is a powerful tool for studying chromatin dynamics and protein-DNA interactions. It is based on the principle that antibodies can be used to target specific proteins bound to DNA. Once the target protein is bound by the antibody, pAG-Tn5 Transposase is used to integrate DNA adapters into the genomic DNA at the nearby nucleosome. The DNA adapters can then be used to amplify and sequence the genomic DNA, providing insights into the chromatin landscape and protein-DNA interactions at specific loci.