GMP Poly(A) Polymerase
GMP Poly(A) Polymerase is the purest and most reliable Poly(A) Polymerase available, making it the ideal choice for a wide range of RNA research applications. With its high efficiency and specificity, GMP Poly(A) Polymerase can efficiently add poly(A) tails to RNA transcripts of any length, without damaging other biomolecules.
GMP Poly(A) Polymerase: The Purest and Most Reliable Poly(A) Polymerase for Your RNA Research
Poly(A) Polymerase (GMP-grade) is a highly purified enzyme that catalyzes the addition of a poly(A) tail to the 3' end of RNA. Poly(A) tails are essential for RNA stability, translation, and splicing. GMP Poly(A) Polymerase is produced under the strictest Good Manufacturing Practices (GMP) guidelines, ensuring the highest quality and purity.
GMP Poly(A) Polymerase offers a number of benefits for RNA research, including:
- High purity and reliability: GMP Poly(A) Polymerase is the purest and most reliable Poly(A) Polymerase available, ensuring consistent and reproducible results.
- High efficiency and specificity: GMP Poly(A) Polymerase can efficiently add poly(A) tails to RNA transcripts of any length, without damaging other biomolecules.
- Versatility: GMP Poly(A) Polymerase is compatible with a wide range of buffers and reagents, making it ideal for use in a variety of RNA research applications.
GMP Poly(A) Polymerase is used in a wide range of RNA research applications, including:
- mRNA vaccine production: GMP Poly(A) Polymerase is used to add poly(A) tails to mRNA transcripts during the production of mRNA vaccines. This ensures that the mRNA transcripts are stable and can be efficiently translated into proteins by the body.
- In vitro transcription: GMP Poly(A) Polymerase is used to add poly(A) tails to RNA transcripts during in vitro transcription reactions. This ensures that the RNA transcripts are stable and can be used for downstream applications, such as RNA sequencing and protein expression.
- RNA probe preparation: GMP Poly(A) Polymerase is used to add poly(A) tails to RNA probes during RNA probe preparation. This ensures that the RNA probes are stable and can be used accurately to detect and quantify specific RNA targets.
- RNA sequencing: GMP Poly(A) Polymerase is used to add poly(A) tails to RNA transcripts during RNA sequencing library preparation. This ensures that the RNA transcripts are stable and can be efficiently sequenced.
- Proteomics: GMP Poly(A) Polymerase is used to add poly(A) tails to RNA transcripts during proteomics workflows, such as ribosome profiling and RNA-protein interaction studies. This ensures that the RNA transcripts are stable and can be accurately analyzed.
If you are working with RNA in your research, GMP Poly(A) Polymerase is an essential tool for ensuring the stability, quality, and reliability of your results.
Order your GMP Poly(A) Polymerase today and experience the difference!
Poly(A) Polymerase (GMP-grade) - Advancing RNA Modification
Poly(A) polymerase, also recognized as PolyA tailing enzyme, stands as a catalyst for the meticulous addition of AMP, derived from ATP, to the 3' end of single-stranded RNA in a template-independent fashion, culminating in the formation of a Poly(A) tail. Distinguished by its adeptness, Poly(A) polymerase exhibits compatibility with various single-stranded RNAs as substrates. However, it refrains from entertaining DNA as a substrate, and caution is advised against deploying double-stranded RNA and oligonucleotides that fall beneath a certain length as substrates for this intricate process. Importantly, the catalytic Poly(A) tailing reaction exclusively employs ATP, dismissing the usage of ADP or dATP.
Product Features:
- Exceptional template compatibility
- Elevated efficiency coupled with robust stability
- Compliant with GMP standards for reliable production
Product Application:
- Precision Poly(A) tailing for refined RNA modification
Product Manual
| Product Name | Poly(A) Polymerase (GMP-grade) |
|---|---|
| Source | Recombinant expression in Escherichia coli |
| Catalog Number | CSB-DEM084 |
| Physical Form | Liquid |
| Enzyme Activity | 5 U/L |
| Storage Conditions | -20±5°C |
| Storage Buffer | 20mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerol |
| Activity Definition | In a 20 uL system, the amount of enzyme required to allow 1 nmol of AMP to be incorporated into RNA for 10 min at 37°C is 1 unit. |
| Shelf Life | 24 months |
Product Description:
Poly(A) Polymerase, also recognized as Poly(A) Polymerase, facilitates the addition of AMP, derived from ATP, to the 3' end of single-stranded RNA, resulting in the formation of a template-independent Poly(A) tail. This enzyme, expressed in Escherichia coli, exhibits versatility by utilizing various single-stranded RNAs as substrates, excluding DNA from this reaction. Double-stranded RNA and short oligonucleotides are discouraged as substrates. The catalyzed Poly(A) tail reaction exclusively employs ATP, excluding ADP or dATP. The incorporation of CTP and UTP is less than 5% of ATP, and GTP cannot be added to the 3' end of RNA. This GMP-grade Poly(A) Polymerase ensures precision and reliability for your RNA modification needs.
| Product Components | Specifications |
|---|---|
| 1 mRNA Cap 2´-O-Methyltransferase (GMP-grade) | 10KU, 50KU, 250KU |
| 2 10× Capping Reaction Buffer | 0.4mL, 2mL, 10mL |
Operating Protocol
System Configuration:
- Utilize the 10× reaction buffer for optimal performance.
- Incorporate ATP (10 mM/μL) into the reaction mixture to facilitate the process.
- Enhance stability with RNase Inhibitor (40 U/μL).
- Introduce RNA (0.5-10 μg) as the target substrate.
- Employ E. coli Poly(A) Polymerase (5 U/μL) to catalyze the reaction.
- Achieve the desired volume with ddH2O.
Reaction Conditions:
- Execute the reaction at a controlled temperature of 37°C for a duration of 30 minutes.
Termination Steps:
- Cease the reaction by either introducing EDTA to achieve a final concentration of 10 mM or conducting an incubation step at 65°C for 20 minutes.