T7 RNA Polymerase(GMP-grade)

GMP-grade T7 RNA Polymerase: Elevate your molecular biology applications with precision and reliability. Our T7 RNA Polymerase, produced under stringent GMP standards, ensures superior performance in transcription processes, meeting the highest quality and purity requirements for your research and production needs.

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T7 RNA Polymerase (GMP-grade): The Ultimate Solution for High-Quality RNA Synthesis

Introducing our GMP-grade T7 RNA Polymerase: 

Crafted from recombinant protein expression in E. coli, this specialized enzyme facilitates precise RNA synthesis. Utilizing double-stranded DNA containing the T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') as a template and NTP as a substrate, it proficiently generates RNA complementary to the reverse single-stranded DNA downstream of the promoter. Notably, T7 RNA Polymerase exhibits versatility by accepting various DNA substrates, including double-stranded linear blunt ends or 5' protruding ends. This adaptability allows the use of linear plasmids and PCR products as templates for in vitro RNA synthesis.

Key Features:
  • High purity and exceptional transcription activity.
  • Specific recognition of the T7 promoter.
  • Devoid of nuclease and protease contaminants.
  • Manufactured under stringent GMP-compliant production standards.

Enhance your RNA synthesis endeavors with a reliable and precise tool, suitable for a spectrum of applications, including the efficient utilization of linear plasmids and PCR products as templates for in vitro RNA synthesis.

Product NameT7 RNA Polymerase (GMP-grade)
SourceBovine Pancreas
Catalog NumberCSB-DEM078
Physical FormLiquid
Enzyme Activity50 U/μL
Storage Conditions-20±5°C
Storage Buffer20 mM sodium acetate (pH 6.5), 5 mM CaCl2, 0.1 mM PMSF, 50% glycerol
Activity DefinitionThe enzyme amount required to incorporate 1 nmol of [3H] GMP into acid-insoluble precipitate within 1 hour at 37°C, pH 8.0, is defined as 1 unit.
Shelf Life12 months

Product Description:

This product is a recombinant T7 RNA polymerase expressed in Escherichia coli. It efficiently synthesizes RNA complementary to the downstream region of a double-stranded DNA template containing the T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') using NTPs as substrates. The enzyme exhibits versatility by accepting both linear blunt-ended and 5' overhang DNA as substrates, enabling the use of linear plasmids and PCR products as templates for in vitro RNA synthesis.

Product Components:

1T7 RNA Polymerase5KU, 25KU, 50KU
210× Transcription Buffer0.4 mL, 2 mL, 4 mL

Operating Instructions:

The reaction system should be prepared as follows:

ComponentsVolume (μL)
10× Transcription Buffer2
CTP / GTP / ATP / UTP (100 mM each)0.4 each
T7 RNA Polymerase (50 U/μL)0.5-1
RNase Inhibitor (40 U/μL)1
RNase-free H2OUp to 18
Template DNA2 (100 ng-1μg)