T7 RNA Polymerase（GMP-grade）
GMP-grade T7 RNA Polymerase: Elevate your molecular biology applications with precision and reliability. Our T7 RNA Polymerase, produced under stringent GMP standards, ensures superior performance in transcription processes, meeting the highest quality and purity requirements for your research and production needs.
Order your T7 RNA Polymerase (GMP-grade) today and experience the difference!
T7 RNA Polymerase (GMP-grade): The Ultimate Solution for High-Quality RNA Synthesis
Introducing our GMP-grade T7 RNA Polymerase:
Crafted from recombinant protein expression in E. coli, this specialized enzyme facilitates precise RNA synthesis. Utilizing double-stranded DNA containing the T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') as a template and NTP as a substrate, it proficiently generates RNA complementary to the reverse single-stranded DNA downstream of the promoter. Notably, T7 RNA Polymerase exhibits versatility by accepting various DNA substrates, including double-stranded linear blunt ends or 5' protruding ends. This adaptability allows the use of linear plasmids and PCR products as templates for in vitro RNA synthesis.
Key Features:
- High purity and exceptional transcription activity.
- Specific recognition of the T7 promoter.
- Devoid of nuclease and protease contaminants.
- Manufactured under stringent GMP-compliant production standards.
Applications:
Enhance your RNA synthesis endeavors with a reliable and precise tool, suitable for a spectrum of applications, including the efficient utilization of linear plasmids and PCR products as templates for in vitro RNA synthesis.
| Specifications | Description |
|---|---|
| Product Name | T7 RNA Polymerase (GMP-grade) |
| Source | Bovine Pancreas |
| Catalog Number | CSB-DEM078 |
| Physical Form | Liquid |
| Enzyme Activity | 50 U/μL |
| Storage Conditions | -20±5°C |
| Storage Buffer | 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, 0.1 mM PMSF, 50% glycerol |
| Activity Definition | The enzyme amount required to incorporate 1 nmol of [3H] GMP into acid-insoluble precipitate within 1 hour at 37°C, pH 8.0, is defined as 1 unit. |
| Shelf Life | 12 months |
Product Description:
This product is a recombinant T7 RNA polymerase expressed in Escherichia coli. It efficiently synthesizes RNA complementary to the downstream region of a double-stranded DNA template containing the T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') using NTPs as substrates. The enzyme exhibits versatility by accepting both linear blunt-ended and 5' overhang DNA as substrates, enabling the use of linear plasmids and PCR products as templates for in vitro RNA synthesis.
Product Components:
| Label | Components | Specifications |
|---|---|---|
| 1 | T7 RNA Polymerase | 5KU, 25KU, 50KU |
| 2 | 10× Transcription Buffer | 0.4 mL, 2 mL, 4 mL |
Operating Instructions:
The reaction system should be prepared as follows:
| Components | Volume (μL) |
|---|---|
| 10× Transcription Buffer | 2 |
| CTP / GTP / ATP / UTP (100 mM each) | 0.4 each |
| T7 RNA Polymerase (50 U/μL) | 0.5-1 |
| RNase Inhibitor (40 U/μL) | 1 |
| RNase-free H2O | Up to 18 |
| Template DNA | 2 (100 ng-1μg) |