what is single cell sequencing ? 

Single-cell sequencing is a cutting-edge molecular biology technique that allows researchers  to analyze  the genetic and genomic information of individual cells within a complex biological sample. Unlike traditional sequencing methods that provide information about the average characteristics  of a cell population, single-cell sequencing offers insights into the genetic and functional diversamong individual cells. 

This technology has revolutionized our understanding of cellular heterogeneity  in various biological contexts, such as cancer research, neuroscience, immunology, and developmental biology.  It enables the identification of rare cell types, the study of cell-to-cell variability, and the discovery of novel cell subpopulations. Single-cell sequencing involves the isolation and sequencing of DNA or RNA from individual cells, and it can provide detailed information about gene expression, mutation analysis, epigenetics, and more at a single-cell level. This powerful tool has broad applications in both basic research and clinical studies, offering new perspectives and opportunities for breakthrough discoveries.

1. Single Cell 3' Transcriptome kit

SeekOne DD (Digital Droplet) Single Cell 3' Transcriptome-seq is a high-throughput single-cell RNA-seq library kit achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The kit needs to be used with the SeekOneDigital Droplet System (SeekOneDD) to complete the whole process from single-cell transcriptome barcoding to library construction.

SeekOne DD single cell 3' transcriptome kit includes: SeekOne DD Chip S3 (Chip S3 for short), Gasket, Carrier Oil, SeekOne DD 3' Barcoded Beads (Barcoded Beads for short), amplification reagents, library construction reagents, and single cell data analysis software (SeekSoul Tools). Based on the principle of microfluidic technology, the kit uses emulsion droplets to separate and capture individual cells, and then recruits nucleic acid-modified Barcoded Beads to molecularly label RNA from different cell sources. Finally, a high-throughput single-cell transcriptome library compatible with Illumina and MGI sequencing system will be constructed, which can be used for research on tumors, immunity, cell development, viral infections, drug discovery, and target screening, etc.

 2. Library Construction