Unleash the Power of Protease K Liquid: Your Versatile Solution for Protein Digestion
Protease K Liquid is the ultimate tool for protein digestion, offering unrivaled efficiency and precision. Its unique liquid formulation ensures complete and consistent digestion, making it ideal for a wide range of applications, including protein analysis, sample preparation, and cell lysis. Experience the power of Protease K Liquid and unlock the secrets of your proteins.
Product Introduction
Proteinase K Liquid is a highly efficient and versatile enzyme derived from Tritirachium album Limber. It exhibits robust proteolytic activity, specifically cleaving peptide bonds at the carboxyl end of aliphatic and aromatic amino acids. This unique property makes Proteinase K Liquid an invaluable tool for a wide range of molecular biology applications, including DNA and RNA extraction, protein analysis, and sample preparation.
Product Features
- Unparalleled Batch Stability: Proteinase K Liquid adheres to stringent production processes and quality inspection standards, ensuring minimal batch-to-batch variations. With an inter-batch enzyme activity CV of ≤1.9%, far exceeding industry averages, Proteinase K Liquid delivers consistent performance and reliability.
- Reliable Production Capacity: Our large-scale production system guarantees a monthly production capacity of lyophilized powder exceeding 70Kg. This ensures a steady supply cycle of 3 days, providing long-term stability and eliminating supply chain disruptions.
- Broad Sample Compatibility: Proteinase K Liquid is not only ideal for processing COVID-19 nasopharyngeal swab samples but also effectively extracts DNA and RNA from diverse complex samples, including swine fever virus, influenza virus, HBV, and HCV.
- Customized Solutions: We cater to specific production needs by customizing product form, packaging, and specifications. This eliminates the hassle of dispensing and enhances work efficiency.
Product Applications
- RNA and DNA extraction: Proteinase K Liquid is a cornerstone reagent for the isolation of high-quality nucleic acids from various biological samples.
- In situ hybridization and other molecular experiments: Proteinase K Liquid's ability to digest proteins makes it an essential tool for in situ hybridization and other molecular techniques that require tissue permeabilization.
Product Data
Calibration Curve
Standard solutions of L-tyrosine at different concentrations were prepared and their absorbance (A) at 275nm was directly measured using a UV spectrophotometer. A calibration curve was generated by plotting absorbance A (ordinate) against tyrosine concentration (abscissa). Based on the calibration curve, the specific enzyme activity was calculated to be ≥30 U/mg.
With its exceptional stability, broad sample compatibility, and customizable options, Proteinase K Liquid stands as a premium choice for researchers seeking a versatile and reliable proteolytic enzyme.
Purity
The protein concentration is detected by SDS-PAGE electrophoresis. The purity of Proteinase K produced by our company is above 95%, with a size of 28.9kDa.
Stability
Proteinase K powder was stored at 4°C for one year, and enzyme activity was measured every month. The results are shown in the figure below, indicating that Proteinase K has good stability and enzyme activity is maintained above 95%.
| Property | Value |
|---|---|
| Product Name | Proteinase K |
| Catalog Number | CSB-DP437A |
| Presentation | Liquid, dissolved in Tris-HCl, pH 7.5-8.0, 10% glycerol, 0.02-0.2% NaN3 |
| Source | Recombination (Pichia) |
| Notice | 1. Molecular biology grade of Proteinase K is not a sterile preparation. If used for cellular or sterile experiments, please pre-treat with 0.22μm filter for sterilization. 2. Please wear a mask, lab-gown, and disposable gloves for your safety. 3. Recommend using low protein adsorption polyethersulfone (PES) or polyvinylidene fluoride (PVDF) membranes for all sterilization processes. |
| Storage | Aliquot and store at 2-8 ℃. Avoid repeated freeze/thaw cycles. For long-term storage, please keep it at -20°C. |
| Valid Period | 2-8℃ for 18 months, -20℃ for 2 years |
| Purity | 90%±5% by SDS-PAGE |
| Application | Genomic DNA Purification Kit, RNA Purification Kit, Gene diagnostic kit, IHC, ISH, and other possible applications |
Proteinase K Liquid: A Superior Proteolytic Enzyme for Molecular Biology Applications
Product Overview
Proteinase K Liquid is a highly purified, concentrated solution of Proteinase K, a serine protease derived from the fungus Tritirachium album Limber. It is a non-specific protease that cleaves peptide bonds at the carboxyl side of aliphatic and aromatic amino acids, making it a valuable tool for a wide range of molecular biology applications. Proteinase K Liquid is known for its exceptional stability, broad sample compatibility, and high enzymatic activity, making it a preferred choice for researchers seeking a reliable and versatile proteolytic enzyme.
Key Features
- Unparalleled Stability: Proteinase K Liquid exhibits remarkable stability over a wide range of temperatures and pH conditions, ensuring consistent performance and minimizing degradation.
- Exceptional Enzyme Activity: Proteinase K Liquid boasts high enzymatic activity, enabling efficient protein digestion and sample preparation.
- Broad Sample Compatibility: Proteinase K Liquid effectively degrades proteins from diverse biological samples, including animal tissues, plant tissues, and microorganisms.
- Ideal for DNA and RNA Extraction: Proteinase K Liquid is a crucial reagent for extracting high-quality nucleic acids from various samples, making it a cornerstone of molecular biology protocols.
Applications
Proteinase K Liquid finds widespread application in various molecular biology techniques, including:
- DNA and RNA Extraction: Proteinase K Liquid efficiently lyses cells and degrades proteins, allowing for the isolation of pure nucleic acids for downstream analysis.
- Protein Analysis: Proteinase K Liquid can be used to digest proteins, facilitating protein identification and characterization.
- Sample Preparation: Proteinase K Liquid is employed in sample preparation for various molecular biology techniques, such as in situ hybridization and blotting.
Product Specifications
- Activity: ≥30 U/mg
- Storage: -20°C
- Stability: Stable at room temperature for up to 1 year
- Form: Lyophilized powder
- Packaging: Various sizes available
Customized Solutions
We cater to specific research needs by offering customized solutions for Proteinase K Liquid, including:
- Customized Enzyme Activity: Tailored enzyme activity levels to meet specific requirements
- Customized Buffer Formulations: Buffer formulations optimized for specific applications
- Customized Packaging: Customized packaging options to suit individual preferences
Proteinase K Liquid stands as a superior proteolytic enzyme, offering exceptional stability, broad sample compatibility, and high enzymatic activity. Its versatility and reliability make it an indispensable tool for researchers across various molecular biology disciplines. With its customizable options, Proteinase K Liquid can be tailored to meet the specific needs of individual research projects.