Product Overview
Elevate your research capabilities with our sophisticated pAG-Tn5 Transposase, a cutting-edge fusion enzyme meticulously engineered by combining Protein A/G with a modified and highly active Tn5 transposase. This fusion marvel seamlessly integrates the functions of both transposase and Protein A/G, rendering it an ideal choice for applications in CUT&Tag technology, specifically tailored for protein-genome interaction studies.
CUT&Tag stands as a paradigm shift in the exploration of protein-DNA interactions. Embrace efficiency with advantages such as time optimization, minimal cell requirements, low background signal, impeccable reproducibility, and even adaptability to single-cell sequencing. This powerful methodology becomes your indispensable tool for probing protein-chromatin interactions in vivo, unraveling intricate details pivotal to the realms of gene regulation and epigenetics.
Key Features:
- Fusion of Protein A/G with a modified Tn5 transposase.
- Dual functionality: transposase and Protein A/G activities.
- Tailored for CUT&Tag technology, revolutionizing protein-genome interaction studies.
Advantages of CUT&Tag:
- Time-efficient and minimizes cell requirements.
- Low background signal and exceptional reproducibility.
- Applicable to single-cell sequencing, broadening research possibilities.
Applications
Delve into the intricate realm of protein-chromatin interactions in vivo with our innovative tools. Uncover the mysteries of gene regulation and delve deep into the fascinating field of epigenetics. Our products empower your research, offering insights that contribute significantly to advancing the understanding of complex biological processes. Explore new dimensions in molecular studies with precision and efficiency, opening doors to novel discoveries in your scientific journey.
Unveil the Potential:
The Protein A/G component within this exceptional product showcases its prowess by primarily interacting with the Fc region of immunoglobulins, establishing versatile binding capabilities across a spectrum of mammalian IgGs. Choose pAG-Tn5 Transposase for a journey into unparalleled precision and innovation, as you navigate the intricacies of molecular exploration.
Product Components
| Label | Component | Specifications |
|---|---|---|
| 1 | pAG-Tn5 Transposome (4 µM) | 12µL (per reaction) |
| 48µL (total for 4 reactions) |
Revolutionary Introduction to pAG-Tn5 Transposome
Embark on a transformative journey with our pAG-Tn5 Transposome, an ingenious fusion uniting Protein A/Protein G with a highly potent mutant variant of Tn5 transposase. This groundbreaking amalgamation endows the transposome with dual functionality, seamlessly integrating the attributes of Tn5 transposase and Protein A/Protein G. Engineered to redefine genomic studies, this exceptional product finds its niche in the realm of CUT&Tag, unraveling the intricacies of protein-DNA interactions with unprecedented efficiency.
Noteworthy Features:
- Meticulously purified through multiple rounds of column chromatography, SDS-PAGE gel analysis reveals a solitary, well-defined target band.
- Rigorous PCR scrutiny ensures the absence of residual host DNA, guaranteeing the purity of your research.
- Shielded from contamination by endonucleases or exonucleases, ensuring the integrity of your experimental outcomes.
Versatile Applications
Elevate your exploration of protein-DNA interactions to unprecedented levels, unlocking new dimensions in your research endeavors. Seamlessly facilitate the construction of second-generation sequencing libraries with unparalleled precision, enhancing the efficiency of your genomic studies. Harness the power of our tools in ATAC-seq applications, extending the boundaries of your genomic explorations. Our versatile solutions empower your scientific pursuits, providing a comprehensive toolkit to address diverse challenges in the ever-evolving landscape of molecular research.
Product Integrity:
Seize the reins of confidence as SDS-PAGE electrophoresis attests to the impeccable quality of our pAG-Tn5 Transposome, setting a new standard in molecular research. Elevate your experiments with a product that not only promises innovation but delivers it with unmatched precision.
| Property | Description |
|---|---|
| Product Name | pAG-Tn5 Transposome |
| Source | Recombinant expression in Escherichia coli |
| Catalog Number | CSB-DEM071 |
| Physical Form | Liquid |
| Storage Conditions | -20°C |
| Molecular Weight | 75 kDa |
| Storage Buffer | 50mM HEPES (pH 7.2), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.1% Triton X-100, 50% (v/v) Glycerol |
| Quality Control | No residual nucleases and exonucleases |
| Shelf Life | 12 months |