WSE-7110 EzWestLumiOne: A Highly Sensitive Luminescence Substrate for HRP  

WSE-7110 EzWestLumiOne: A Superior Luminescence Substrate for HRP Detection

In the realm of protein research, Western blotting stands as a cornerstone technique for detecting and analyzing proteins. However, the sensitivity of traditional HRP (horseradish peroxidase) detection substrates often limits the ability to visualize low-abundance proteins. To address this challenge, ATTO Corporation has developed WSE-7110 EzWestLumiOne, a highly sensitive luminescence substrate that revolutionizes Western blotting with its exceptional performance.

Unveiling Low-Abundance Proteins with Enhanced Sensitivity

EzWestLumiOne boasts remarkable sensitivity, outperforming traditional ECL substrates by up to 100 times. This enhanced sensitivity empowers researchers to detect proteins present at low concentrations, providing a more comprehensive view of protein expression patterns.

Sustained Luminescence for Extended Exposure

EzWestLumiOne emits a long-lasting luminescence signal that remains stable for up to an hour, allowing for extended exposure times. This extended luminescence ensures the capture of even faint protein bands, leading to more complete protein profiles.

Broad Dynamic Range for Accurate Detection

The substrate exhibits a wide dynamic range, enabling the accurate detection of proteins across a broad range of concentrations. This wide dynamic range eliminates the need to adjust exposure times for different protein levels, simplifying the Western blotting process.

Improved Linearity for Reliable Quantification

EzWestLumiOne produces a linear signal response over a broad range of protein concentrations, facilitating reliable protein quantification. This linearity allows researchers to accurately determine relative protein abundance, providing valuable insights into protein function and regulation.

Streamlined Workflow for Enhanced Efficiency

EzWestLumiOne is a ready-to-use solution, eliminating the need for mixing or incubation steps. This single-component formulation simplifies the Western blotting workflow, saving time and reducing the risk of errors.

Applications:

• Western blotting: EzWestLumiOne is the ideal substrate for Western blotting, enabling the detection of a wide range of proteins, including low-abundance proteins.
• ELISA: The substrate can also be used in ELISA (enzyme-linked immunosorbent assay) applications, providing a sensitive method for quantifying proteins in solution.
• Dot blot: EzWestLumiOne can be used in dot blot assays to detect proteins immobilized on membranes.
• Immunoassays: The substrate is suitable for various immunoassay applications, including those involving protein-protein interactions and protein-antibody binding.

WSE-7110 EzWestLumiOne is an indispensable tool for Western blotting, enabling researchers to:

• Detect low-abundance proteins: Uncover subtle changes in protein expression by detecting proteins present at low levels.
• Visualize protein bands clearly: Capture faint protein bands with confidence due to the substrate's long-lasting luminescence.
• Quantify protein levels accurately: Perform reliable protein quantification by taking advantage of the substrate's linear signal response.
• Simplify the Western blotting workflow: Streamline the detection process with the substrate's ready-to-use formulation.

Elevate Your Western Blotting with EzWestLumiOne:

WSE-7110 EzWestLumiOne revolutionizes Western blotting by providing enhanced sensitivity, extended luminescence, and improved linearity. By employing this innovative substrate, researchers can gain deeper insights into protein expression patterns and perform more accurate protein quantification, advancing their research endeavors.

Benefits:

• Increased sensitivity: EzWestLumiOne can detect lower levels of HRP than traditional ECL substrates, making it ideal for detecting low-abundance proteins.
• Improved signal-to-noise ratio: The high sensitivity and long-lasting signal of EzWestLumiOne result in a high signal-to-noise ratio, making it easy to visualize proteins of interest.
• Reduced exposure time: The high sensitivity of EzWestLumiOne allows for shorter exposure times, reducing the risk of overexposure and film fogging.
• Simplified workflow: EzWestLumiOne is a ready-to-use solution that eliminates the need for mixing or incubation steps, simplifying the workflow.

Conclusion:

WSE-7110 EzWestLumiOne stands as a groundbreaking luminescence substrate for HRP detection, offering a combination of enhanced sensitivity, sustained luminescence, broad dynamic range, improved linearity, and a streamlined workflow. By employing this innovative substrate, researchers can gain deeper insights into protein expression patterns, perform accurate protein quantification, and streamline their Western blotting protocols. EzWestLumiOne truly revolutionizes protein research, empowering scientists to make significant advancements in their respective fields.