GMP Inorganic Pyrophosphatase: A Pure and Efficient Catalyst for Pyrophosphate Hydrolysis

Pyrophosphatase, Inorganic (GMP-grade) - Unveiling Enhanced Catalysis

Discover the prowess of our inorganic pyrophosphatase, meticulously derived through recombinant expression in E. coli. This enzyme serves as a catalytic marvel, orchestrating the conversion of one pyrophosphate molecule into two phosphate ions. Exploiting the high-energy nature of this reaction, it effectively propels thermodynamically unfavorable transformations to completion. In the realm of molecular biology, our inorganic pyrophosphatase (PPase) emerges as a catalyst for the hydrolysis of inorganic pyrophosphate, yielding valuable orthophosphate.

Product Highlights:

• Versatile activity across various reaction buffers.
• Operational efficiency at temperatures ranging from 16 to 37°C, with a convenient inactivation option at 65°C for 10 minutes.
• Compliant with GMP standards, ensuring a quality production system.

Applications:

• Direct integration into experiments like HDA amplification and LAMP amplification.
• Empowers the augmentation of RNA yield in reverse transcription reactions, demonstrating its multifaceted utility.

• Product Name: Inorganic Pyrophosphatase
  
  
• Source: Recombinant expression in yeast
  
  
• Catalog Number: CSB-DEM081
  
  
• Physical Form: Liquid
  
  
• Enzyme Activity: 0.1 U/μL
  
  
• Storage Conditions: -20±5°C
  
  
• Molecular Weight: 71 kDa
  
  
• Storage Buffer: 50 mM Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% Triton X-100, 50% glycerol
  
  
• Activity Definition: Under standard reaction conditions, the amount of enzyme required to catalyze the hydrolysis of PPI to produce 1 μmol Pi per minute is defined as 1 unit (standard reaction contains 100 mM Tris-HCl pH 7.2, 2 mM MgCl2, 2 mM Ppi in a 500 μL system; reaction performed at 25℃ for 10 minutes)
  
  
• Shelf Life: 12 months
  

Product Description:

Unleash the power of inorganic pyrophosphatase, a product meticulously crafted through the recombinant artistry of Escherichia coli. This enzyme orchestrates the remarkable transformation of one pyrophosphate molecule into two phosphate ions, a process brimming with exergonic brilliance. Its ability to drive thermodynamically challenging conversions to completion sets the stage for molecular feats. In the realm of molecular biology, inorganic pyrophosphatase (PPase) gracefully conducts the hydrolysis of inorganic pyrophosphate, producing the coveted orthophosphate. A virtuoso in enhancing RNA yield, it gracefully takes center stage in reverse transcription reactions.

Product Components:

Pyrophosphatase, Inorganic (GMP-grade) is a highly purified enzyme that catalyzes the hydrolysis of pyrophosphate (PPi) to inorganic phosphate (Pi). It is produced under the strictest Good Manufacturing Practices (GMP) guidelines, ensuring the highest quality and purity.

PPi is a byproduct of many biochemical reactions, including DNA synthesis, RNA transcription, protein phosphorylation, and energy metabolism. The accumulation of PPi can inhibit these reactions, so it is important to remove PPi from biochemical reactions to ensure their optimal performance.

GMP Inorganic Pyrophosphatase works by binding to PPi and hydrolyzing it to Pi. This reaction is irreversible, and it removes PPi from the solution. GMP Inorganic Pyrophosphatase is a very efficient enzyme, and it can completely hydrolyze PPi in a matter of minutes.

GMP Inorganic Pyrophosphatase is used in a variety of research and clinical applications, including:

• PCR: GMP Inorganic Pyrophosphatase is used to remove PPi from PCR reactions, improving the yield and quality of PCR products.
• In vitro transcription: GMP Inorganic Pyrophosphatase is used to remove PPi from in vitro transcription reactions, improving the yield and quality of RNA transcripts.
• DNA sequencing: GMP Inorganic Pyrophosphatase is used to remove PPi from DNA sequencing reactions, improving the accuracy and reliability of the sequencing data.
• Protein phosphorylation assays: GMP Inorganic Pyrophosphatase is used to remove PPi from protein phosphorylation assays, improving the sensitivity and specificity of the assays.

GMP Inorganic Pyrophosphatase is also used in a variety of industrial applications, such as food processing and biotechnology.

Benefits of GMP Inorganic Pyrophosphatase:

• High efficiency and specificity: GMP Inorganic Pyrophosphatase completely hydrolyzes PPi in a matter of minutes, and it does not bind to other biomolecules.
• Broad range of activity: GMP Inorganic Pyrophosphatase is active at a wide range of temperatures and pH values.
• High stability: GMP Inorganic Pyrophosphatase is stable in storage for long periods of time.
• Low toxicity: GMP Inorganic Pyrophosphatase is non-toxic and can be used safely in a variety of applications.

GMP Inorganic Pyrophosphatase is essential for a wide range of research and clinical applications, including:

• Pyrophosphate removal from PCR reactions: Pyrophosphate is a byproduct of PCR reactions and can inhibit the activity of DNA polymerase. GMP Inorganic Pyrophosphatase is used to remove pyrophosphate from PCR reactions, improving the yield and quality of PCR products.
• Pyrophosphate removal from in vitro transcription reactions: Pyrophosphate is also a byproduct of in vitro transcription reactions and can inhibit the activity of RNA polymerase. GMP Inorganic Pyrophosphatase is used to remove pyrophosphate from in vitro transcription reactions, improving the yield and quality of RNA transcripts.
• Pyrophosphate removal from other biochemical reactions: Pyrophosphate is a byproduct of many other biochemical reactions, such as DNA synthesis and protein phosphorylation. GMP Inorganic Pyrophosphatase can be used to remove pyrophosphate from these reactions, improving the efficiency and yield of the reactions.

GMP Inorganic Pyrophosphatase is easy to use and can be added to biochemical reactions at any time to remove pyrophosphate. It is also compatible with a wide range of buffers and reagents, making it a versatile tool for your research needs.

Order your GMP Inorganic Pyrophosphatase today and experience the difference!