Enhance PCR Specificity with Anti-Taq DNA Polymerase Antibody: Your Guardian Against Nonspecific Amplification
Elevate the accuracy and reliability of your PCR experiments with Anti-Taq DNA Polymerase antibody, a powerful tool that effectively inhibits non-specific amplification, ensuring precise target detection and enhanced specificity. This innovative antibody safeguards the integrity of your PCR results, making it an indispensable asset for any molecular biology laboratory.
Introduction
Taq enzyme monoclonal antibody is a novel reagent specifically designed for Hot-Start PCR applications. By reversibly inhibiting the activity of Taq DNA polymerase at ambient temperatures, it effectively suppresses non-specific amplification and primer dimer formation, leading to enhanced PCR fidelity and specificity.
Features
- High Sealing Efficiency: Taq antibody exhibits remarkable sealing efficiency, requiring a minimal antibody concentration (fusion ratio: 0.08 μg Taq antibody/1 U Taq enzyme) to achieve an antibody blocking efficiency of no less than 90%.
- Excellent Batch Stability: Large-scale suspension cell production ensures minimal host antibody nucleic acid contamination, resulting in exceptionally high purity and batch-to-batch consistency.
- Broad Applicability: Taq antibody is compatible with a wide range of Taq DNA polymerases, making it a versatile tool for Hot-Start PCR applications.
Applications
- Taq DNA polymerase sealing
- Hot-Start PCR
- Taq enzyme stability enhancement
- Linearity improvement in sealed antibody applications
Product Data
- Sealing effect of different concentrations
The blocking effect of different concentrations of Taq enzyme monoclonal antibody (CSB-DA403AmN①) on Taq DNA Polymerase :
Amplification of human RNaseP gene using Taq DNA Polymerase blocked by Taq enzyme monoclonal antibody (CSB-DA403AmN①), with template gradients of 5ng, 500pg, 50pg, and 5pg, to make a standard curve. It can be seen that R2 is 99.35%, amplification efficiency is 98.957%, linearity is good, and there is no non-specific amplification :
Product Name: Anti-Taq DNA Polymerase antibody
Source: Mouse
Physical Form: Liquid
Enzyme Activity: 5U/µL
Storage Conditions: -20 ±5°C
Storage Buffer: 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween20, 0.5% IGEPAL CA630, 50% Glycerol
Activity Definition: The amount of Taq Antibody that can inhibit more than 90% of 1U unit Taq DNA Polymerase activity under the condition of 55°C for 10 minutes is defined as 1 unit (U) of activity.
Quality Control: No DNase, RNase activity; no exo- and endonuclease activity; PCR testing shows no microbial genomic residue.
Shelf Life: 24 months
Product Description:
Taq Antibody is a monoclonal antibody against Taq DNA polymerase used in hot start PCR. It can bind to Taq DNA polymerase and inhibit its activity, effectively suppressing non-specific annealing of primers and non-specific amplification caused by primer dimers. Taq Antibody can undergo denaturation in the initial DNA pre-denaturation step of PCR reaction, so using this product does not require special Taq Antibody inactivation treatment. Taq DNA polymerase can be used under conventional PCR reaction conditions.
Product Components:
| Component Number | Component Name | Specifications |
|---|---|---|
| 1 | Anti-Taq DNA Polymerase antibody | 500 U, 2500 U, 5000 U |
Anti-Taq DNA Polymerase Antibody: A Powerful Tool for Enhanced PCR Performance
In the realm of molecular biology, Taq DNA Polymerase stands as a cornerstone for polymerase chain reaction (PCR), a ubiquitous technique for amplifying and detecting specific DNA sequences. However, conventional Taq DNA Polymerase exhibits activity at ambient temperatures, leading to non-specific amplification and primer dimer formation, compromising the accuracy and sensitivity of PCR. To address this challenge, the Anti-Taq DNA Polymerase antibody emerges as a valuable reagent for Hot-Start PCR applications.
Mechanism of Action: Reversible Inhibition of Taq DNA Polymerase
The Anti-Taq DNA Polymerase antibody functions by reversibly binding to Taq DNA Polymerase, effectively blocking its enzymatic activity at ambient temperatures. This inhibition prevents non-specific amplification and primer dimer formation, ensuring that PCR amplification only occurs during the thermal cycling stages.
Key Features and Benefits
- High Sealing Efficiency: The antibody exhibits remarkable sealing efficiency, requiring a minimal concentration (fusion ratio: 0.08 μg antibody/1 U Taq enzyme) to achieve an antibody blocking efficiency of no less than 90%.
- Excellent Batch Stability: Large-scale suspension cell production ensures minimal host antibody nucleic acid contamination, resulting in exceptionally high purity and batch-to-batch consistency.
- Broad Applicability: The antibody is compatible with a wide range of Taq DNA polymerases, making it a versatile tool for Hot-Start PCR applications.
- Enhanced PCR Specificity and Sensitivity: By suppressing non-specific amplification and primer dimer formation, the antibody contributes to improved PCR specificity and sensitivity, leading to more accurate and reliable results.
Applications in Molecular Biology
- Hot-Start PCR: The antibody plays a pivotal role in Hot-Start PCR, enabling specific DNA amplification with minimal background noise.
- Taq DNA Polymerase Sealing: The antibody effectively seals Taq DNA Polymerase, preventing unwanted enzymatic activity at ambient temperatures.
- PCR Fidelity Enhancement: The antibody promotes PCR fidelity by reducing non-specific amplification and primer dimer formation.
- Linearity Improvement: The antibody contributes to improved PCR linearity, ensuring accurate quantification of DNA targets.
Safety Considerations
- Handling and Storage: The antibody should be handled with appropriate care, following standard laboratory practices. Store the antibody at the recommended temperature as per the manufacturer's instructions.
- Disposal: Dispose of the antibody and contaminated waste according to institutional guidelines and environmental regulations.
Conclusion
The Anti-Taq DNA Polymerase antibody stands as an invaluable reagent for Hot-Start PCR applications, offering a combination of high sealing efficiency, excellent batch stability, and broad applicability. By effectively suppressing non-specific amplification and primer dimer formation, the antibody enhances PCR specificity, sensitivity, and linearity, making it an essential tool for accurate and reliable molecular biology research.