Tn5 Transposase

Revolutionizing Genomic Exploration with Tn5 Transposase

Discover the cutting-edge efficiency of Tn5 Transposase, an exceptionally active mutant variant meticulously crafted from E. coli. Engineered for precision, it seamlessly inserts Tn5 transposons into target sequences, showcasing unparalleled transposition insertion efficiency for both eukaryotic and prokaryotic DNA.

Tn5 Transposase's prowess lies in its specific recognition of DNA fragments featuring chimeric end sequences, culminating in the formation of dynamic Tn5 transposomes. These transposomes intricately bind to target DNA, executing precise cleavage and insertion of their cargo DNA fragments.

A versatile tool in the realm of genomics, this product plays a pivotal role in constructing second-generation sequencing library fragments. It excels in the fragmentation and adapter ligation steps, paving the way for groundbreaking advancements in genetic exploration.

Embark on a journey of innovation with Tn5 Transposase, unlocking new possibilities in genomics and revolutionizing your approach to genetic research.

Tn5 transposase refers to the transposase enzyme associated with the Tn5 transposon. Transposons, also known as "jumping genes" or "mobile genetic elements," are segments of DNA that can move from one location in a genome to another. They play a significant role in genome evolution and can contribute to genetic diversity.

Here are some key points about Tn5 transposase and the Tn5 transposon:

1. Function: The primary function of Tn5 transposase is to catalyze the movement of the Tn5 transposon within a DNA molecule. It achieves this by recognizing specific sequences at the ends of the transposon (known as inverted repeats) and catalyzing the excision and integration of the transposon into a new target site.
2. Tn5 Transposon Structure: The Tn5 transposon is a bacterial transposon originally identified in Escherichia coli (E. coli). It typically consists of genes encoding transposase, as well as other accessory genes that may confer selective advantages to the host bacterium.
3. Inverted Repeats: The ends of the Tn5 transposon contain inverted repeats, which are specific DNA sequences that are recognized by the Tn5 transposase. These sequences are essential for the transposition process.
4. Transposition Process: The transposition process involves the excision of the Tn5 transposon from its original location in the genome and the insertion into a new site. Tn5 transposase catalyzes the cleavage of the transposon ends and promotes the integration into the target DNA.
5. Applications in Molecular Biology: Tn5 transposase has been widely used as a tool in molecular biology research. Modified versions of Tn5 transposase, such as the hyperactive Tn5 transposase, have been employed in techniques like Nextera DNA Library Preparation. This method allows for the efficient and simultaneous fragmentation and tagging of DNA, facilitating high-throughput DNA sequencing library preparation.
6. Research Significance: Tn5 transposase and the Tn5 transposon have been extensively studied not only for their role in bacterial genome dynamics but also for their applications in genetic engineering and biotechnology.

Researchers continue to explore the mechanisms of transposition and the potential uses of transposases in various genetic manipulation techniques. The study of transposons provides valuable insights into the molecular processes that drive genetic diversity and evolution in organisms.

Tn5 Transposase: Mechanisms, Evolution, and Applications in Molecular Biology

1. Mechanism of Transposition:

• The Tn5 transposase operates through a cut-and-paste mechanism.
• Identification of inverted repeat sequences initiates cleavage, and the transposase facilitates integration into a new target site.
• Molecular details involve the formation of a transposase-transposon complex, coordinating DNA cleavage and strand transfer reactions.

2. Structural Features:

• Tn5 transposase, a protein with distinct structural domains.
• The catalytic domain is crucial for enzymatic activities related to DNA cleavage and integration.
• Structural integrity is vital for specificity in recognizing transposon ends.

3. Transposon Evolution:

• Tn5 transposons, part of the IS4 family, identified in various bacterial species.
• Evolutionary studies showcase the diversity of transposons and their role in shaping bacterial genomes.
• Transposon movement contributes to genetic variability within bacterial populations.

4. Applications in Genetic Engineering:

• Widely used in genetic engineering and molecular biology.
• Precision in inserting or moving DNA sequences is valuable for gene cloning, mutagenesis, and generating transgenic organisms.

5. Nextera DNA Library Preparation:

• Utilizes Tn5 transposase for streamlined DNA library preparation in next-generation sequencing.
• Simultaneous DNA fragmentation and tagging reduce steps and time requirements.

6. Genomic Tools and Functional Genomics:

• Employed in functional genomics studies to investigate gene function and regulation.
• Integration of transposons with specific genetic elements enables large-scale study of gene function.

7. Site-Specific Mutagenesis:

• Engineered for site-specific mutagenesis.
• Modified transposase versions target specific genomic locations, enabling precise genetic modifications.

8. Biotechnological and Therapeutic Applications:

• Understanding transposition mechanisms and leveraging Tn5 transposase capabilities for biotechnological and gene therapy applications.
• Controlled transposition for targeted gene delivery or modification in therapeutic contexts.

9. Continued Research and Technological Impact:

• Ongoing research on Tn5 transposase advances understanding of transposon biology.
• Contributes to the development of innovative tools for genetic manipulation and genomic studies.
• Tn5-based methods anticipated to play an increasingly crucial role in molecular biology and biotechnology as technology evolves.

Unlocking the Power of Genomic Exploration with Tn5 Transposase

Tn5 transposase, a cutting-edge mutant derived from E. coli, emerges as a powerhouse in genetic research, facilitating precise and efficient Tn5 transposon insertion into target sequences. Its exceptional transposition efficiency spans both eukaryotic and prokaryotic DNA, making it a versatile tool in various applications, from in vitro transgenesis to second-generation sequencing library construction.

Key Features:

• Rigorously purified through multiple column chromatography cycles, ensuring a singular, clear target band in SDS-PAGE gel detection.
• PCR analysis confirms the absence of host DNA residues, guaranteeing the purity of Tn5 transposase.
• Free from contamination by endonucleases or exonucleases, maintaining the integrity of your experiments.

Applications:

• Integral in transposon usage during the fragmentation and adapter addition steps in constructing second-generation sequencing libraries.
• Facilitates in vitro or in vivo construction of random insertion mutant libraries using transposons.
• Enables swift sequencing of substantial DNA molecules, such as BAC clones.
• Empowers the introduction of resistance markers into target DNA for advanced experimentation.

Product Components

Product Data:

Witness the purity and effectiveness of Tn5 transposase through SDS-PAGE electrophoresis.

Unleash the potential of your genetic exploration with Tn5 transposase, the catalyst for innovative breakthroughs in genomics.

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