Streamline DNA/RNA Extraction and Purification with Magnetic Bead Technology
Discover the power of magnetic bead-based technology for efficient and high-quality DNA/RNA extraction and purification. This innovative method offers rapid and reliable separation of nucleic acids from various sample types, making it an essential tool for molecular biology research.
Magnetic bead-based DNA/RNA extraction and purification is a rapid and efficient method for isolating high-quality nucleic acids from a variety of samples, including blood, tissue, cells, and plant material. The method utilizes superparamagnetic beads that have been coated with a specialized surface that selectively binds to DNA or RNA molecules. The beads are then separated from the sample lysate using a magnet, and the nucleic acids are eluted from the beads in a clean buffer.
Principle of Magnetic Bead-Based Nucleic Acid Extraction
The principle of magnetic bead-based nucleic acid extraction is based on the selective binding of nucleic acids to the surface of superparamagnetic beads. The beads are coated with a specialized surface, such as silica or carboxylate, that has a strong affinity for the negatively charged phosphate groups of the nucleic acid backbone. When the sample lysate is added to the beads, the nucleic acids are attracted to the bead surface and bind tightly. The lysate is then washed away, leaving the bound nucleic acids on the beads.
Advantages of Magnetic Bead-Based Nucleic Acid Extraction
- Rapid: Magnetic bead-based nucleic acid extraction is a very rapid method, typically taking less than 30 minutes from start to finish.
- Efficient: The method is highly efficient, with recovery rates of greater than 90% for both DNA and RNA.
- High-quality: The extracted nucleic acids are of high purity and suitable for a wide range of downstream applications, such as PCR, sequencing, and genotyping.
- Easy to use: The method is simple and easy to use, requiring minimal hands-on time and no specialized equipment.
Applications of Magnetic Bead-Based Nucleic Acid Extraction
- Genomic DNA isolation
- Plasmid DNA isolation
- RNA isolation
- Cell-free DNA (cfDNA) isolation
- Viral DNA/RNA isolation
- Forensic DNA analysis
Magnetic bead-based DNA/RNA extraction and purification is a versatile and powerful tool for isolating high-quality nucleic acids from a variety of samples. The method is rapid, efficient, and easy to use, and it is suitable for a wide range of downstream applications.
This innovative extraction kit utilizes high-affinity magnetic particles as the capture matrix. Under high-concentration ionizing agent conditions, these nanomagnetic particles effectively adsorb nucleic acids through hydrogen bonding and electrostatic interactions, while proteins and other impurities remain unbound and are subsequently removed. The nucleic acid-bound nanomagnetic particles are then washed to eliminate proteins and salts, ultimately releasing the purified nucleic acids with a low-salt elution buffer. This entire extraction process operates at room temperature, minimizing the risk of cross-contamination and enhancing the sensitivity and accuracy of downstream applications.
- Capable of extracting high-purity DNA and RNA from diverse sample types, including blood, bacteria, nasopharyngeal swabs, cells, and other liquid samples.
- Adaptable for processing solid samples, such as tissues, following pretreatment and centrifugation of the supernatant.
- The extracted nucleic acids are directly compatible with PCR, fluorescent quantitative PCR, RT-PCR, and other downstream applications.
- Universal extraction of nucleic acids (DNA/RNA) from various sample sources, including viruses, cells, and blood.
- Employs magnetic microspheres, eliminating the need for centrifugation.
- Suitable for both manual extraction of small samples and automated high-throughput operations.
Broad Application Spectrum
- Nucleic acid (DNA/RNA) detection, cloning, sequence analysis, PCR amplification, molecular hybridization, and cDNA synthesis.
|Product Name||DNA/RNA Extraction and Purification Kit (Magnetic Bead Method)|
|Storage Conditions||Kit I: Store at -20±5°C, avoid repeated freeze-thaw cycles. Can be stored at 2-8°C for frequent use. Kit II: Store at 2-25°C|
|Product Components||Proteinase K, Magnetic Beads, Lysis Buffer, Washing Solution, Elution Solution|
|Shelf Life||12 months|
Experience streamlined nucleic acid extraction with our DNA/RNA Extraction and Purification Kit utilizing the advanced Magnetic Bead Method. This reagent kit employs highly binding magnetic particles as the matrix, allowing for efficient adsorption of nucleic acids in high ion concentration conditions through hydrogen bonding and electrostatic adsorption. Proteins and other impurities remain unadsorbed, facilitating their easy removal. The nucleic acid-bound nanoparticles undergo a thorough wash to eliminate proteins and salts, and subsequent elution using a low-salt buffer releases the purified nucleic acids.
This extraction process can be conducted at room temperature, minimizing the risk of cross-contamination and enhancing sensitivity and detection accuracy. Ideal for obtaining high-purity DNA and RNA from diverse samples, including liquid samples such as viruses, bacterial bodies, and cells. For solid samples like tissues, a pre-processing step is required, and the supernatant after suspension centrifugation is also suitable for extraction. The resulting nucleic acids are ready for direct use in PCR, quantitative PCR, RT-PCR, and other applications.
|Kit I||1. Proteinase K Solution||2mL, 4mL, 20mL|
|Kit II||2. Magnetic Bead Suspension||1mL, 2mL, 10mL|
|3. Lysis Buffer||15mL, 30mL, 150mL|
|4. Washing Solution A||50mL, 100mL, 500mL|
|5. Washing Solution B||50mL, 100mL, 500mL|
|6. Elution Solution||5mL, 10mL, 50mL|