Next-generation sequencing
Unlock the secrets of your genome with next-generation sequencing. Explore cutting-edge technology that offers unprecedented insights into DNA, RNA, and epigenetic variations. Revolutionize your genomics research with high-throughput, accuracy, and in-depth analysis. Discover the power of next-generation sequencing for comprehensive and personalized genomic exploration
Next-Generation Sequencing (NGS): Advancing Genomic Analysis
NGS, short for next-generation sequencing, represents a cutting-edge, massively parallel sequencing technology that provides unprecedented levels of throughput, scalability, and rapidity. Its primary function involves the determination of nucleotide sequences within complete genomes or specific segments of DNA or RNA. This transformative technology has reshaped the landscape of biological sciences, empowering laboratories to undertake an extensive array of applications and explore biological systems at a depth previously unattainable.
In addressing contemporary intricate genomics inquiries, the demands for information have surpassed the capabilities of conventional DNA sequencing methods. NGS has effectively bridged this gap, emerging as an indispensable tool for delving into these intricate scientific questions.
Applications of Next-Generation Sequencing (NGS)
The advent of next-generation sequencing (NGS) has substantially expanded the scope of inquiries that researchers can pursue. Leveraging innovative sample preparation techniques and advanced data analysis, NGS has enabled a diverse array of applications. Specifically, NGS facilitates the following capabilities:
- Rapid sequencing of entire genomes
- In-depth sequencing of targeted regions
- Utilization of RNA sequencing (RNA-Seq) for the discovery of novel RNA variants and splice sites, as well as the quantification of mRNAs for gene expression analysis
- Analysis of epigenetic factors, including genome-wide DNA methylation and DNA-protein interactions
- Sequencing of cancer samples to investigate rare somatic variants, tumor subclones, and related aspects
- Study of the human microbiome
- Identification of novel pathogens
The multifaceted nature of NGS applications underscores its indispensable role in contemporary biological research and its potential to further enhance our understanding of complex biological systems.
DNBSEQ-G400RS Performance
| Read Lengths | Data Output/Flow Cell | Data Quality (Q30*) | Run Time (dual-slide mode) |
|---|---|---|---|
| SE50-FCL | 75 Gb ~90 Gb | >90% | ~14 hrs |
| SE100-FCL | 150 Gb ~180 Gb | >90% | ~25 hrs |
| SE400-FCL | 600 Gb ~720 Gb | >70% | ~109 hrs |
| PE100-FCL | 300 Gb ~360 Gb | >85% | ~38 hrs (latest version) |
| PE150-FCL | 450 Gb ~540 Gb | >85% | ~56 hrs (latest version) |
| PE200-FCL | 600 Gb ~720 Gb | >75% | ~107 hrs |
| SE100-FCS | 55 Gb | >85% | ~13 hrs |
| PE100-FCS | 110 Gb | >85% | ~26 hrs |
| PE150-FCS | 165 Gb | >85% | ~37 hrs |
| PE300-FCS | 180 Gb | >80% | ~98 hrs |
- Q30 is a measure of sequencing quality, where a higher percentage of bases above Q30 indicates higher quality data.
- Run time includes sample loading, sequencing, base calling, and data processing.
- The latest version of the DNBSEQ-G400RS has reduced run times for PE100-FCL and PE150-FCL to 38 hrs and 56 hrs, respectively.
The Mechanism of Illumina Next-Generation Sequencing (NGS)
The Illumina NGS technology operates using a distinct methodology compared to the conventional Sanger chain-termination method. It employs the sequencing by synthesis (SBS) technology, which monitors the incorporation of labeled nucleotides while the DNA chain is replicated, in a highly parallel manner.
Next-generation sequencing produces extensive data on DNA sequencing, and it is both more cost-effective and less time-consuming than traditional Sanger sequencing. Illumina sequencing systems can generate data outputs ranging from 300 kilobases up to multiple terabases in a single run, depending on the type and configuration of the instrument.
NovaSeq 6000
The NovaSeq 6000 Sequencing System is designed to adapt to your needs. This robust, scalable system has been adopted by leading research centers and featured in countless publications. Impactful discoveries are within reach.
See All ApplicationsScalable throughput for dynamic study sizes
So many ways to sequence. Scale up and down with a tunable output of up to 6 Tb and 20B single reads in < 2 days.
| System specifications | |
| Output range | 80 - 6000 Gb |
| Paired end reads per run | 1.6B - 40B |
| Max read length | 2 × 250 bp |
| Run time | 13 - 44 hours |
Broad, deep sequencing for clear answers
Accelerate your science with reliable sequencing breadth and depth for every project.
Tunable output for mix and match options
Do what you want. Mix and match flow cell types, and run one or two flow cells at a time. Choose between multiple read lengths, workflows, and more.
Advanced applications. Impactful discoveries.
The NovaSeq 6000 system offers deep and broad coverage through advanced applications for a comprehensive view of the genome. Unlock a full spectrum of genetic variation and biological function with high-throughput sequencing.