GMP DNase I: The Purest and Most Reliable DNase for Your Research Needs

GMP DNase I is the purest and most reliable DNase available, making it the ideal choice for a wide range of research applications. With its high efficiency and specificity, GMP DNase I can completely degrade DNA in minutes, without damaging other biomolecules.

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Product Description:


Deoxyribonuclease I (DNase I), also known as Deoxyribonuclease I, is a nucleic acid enzyme present in various cells and tissues. It is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines, generating polynucleotides with a 5' phosphate group and a 3' hydroxyl group. The average digestion product size is a minimum of tetranucleotides. DNase I can catalyze various forms of DNA, including single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is influenced by histones). The optimal working range is pH 7-8. DNase activity depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, and Zn2+. 5 mM Ca2+ can protect the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cleave at any site on either strand of DNA, while in the presence of Mn2+, it can simultaneously recognize both strands of DNA and cleave at almost the same sites. DNase I was originally isolated from the pancreas and remains one of the major sources of this enzyme in mammals.


Product Components:
LabelComponentsSpecifications
1Deoxyribonuclease I / DNase I (GMP-grade)200 U, 1 KU, 2 KU


Product Manual

Product NameDeoxyribonuclease I / DNase I (GMP-grade)
SourceBovine Pancreas
Catalog NumberCSB-DEM079
Physical FormLiquid
Enzyme Activity2 U/μL
Storage Conditions-20±5°C
Storage Buffer20 mM sodium acetate (pH 6.5), 5 mM CaCl2, 0.1 mM PMSF, 50% glycerol
Activity DefinitionAt 25°C, pH 5.0, when acting on highly polymerized DNA, the amount of enzyme required to increase the absorbance at 260 nm by 0.001 per minute per milliliter is defined as one unit.
Shelf Life24 months


Product Introduction:

DNase I, a versatile nuclease, exhibits the capability to digest both single-stranded and double-stranded DNA, yielding monodeoxynucleotides or single/double-stranded oligodeoxynucleotides. It efficiently hydrolyzes phosphodiester bonds, generating monodeoxynucleotides and oligodeoxynucleotides with 5'-phosphate groups and 3'-OH groups, with the average digestion product size being the smallest tetranucleotide. Catalyzing various forms of DNA, including single-stranded DNA, double-stranded DNA, and chromatin (its cutting rate influenced by histones), DNase I operates optimally within a pH range of 7-8. Its activity is contingent on Ca2+ and can be activated by divalent metal ions like Co2+, Mn2+, Zn2+, etc. The presence of 5mm Ca2+ shields the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cut at any site on any DNA strand; in the presence of Mn2+, it can simultaneously recognize both DNA strands and cut at nearly the same position, forming blunt ends or sticky ends with 1-2 nucleotides protruding.

Product Feature:
  • DNase I can typically be inactivated using heat-based methods. In scenarios necessitating the removal of residual denatured proteins, phenol-chloroform extraction is employed to precipitate RNA samples post-digestion at 37°C (without heating operation).
  • GMP-compliant production system.
Product Application:
  • DNase I finds widespread utility in RNA preparation devoid of DNA; removal of template DNA post in vitro transcription; preparation of DNA-free RNA before RT-PCR and RT-qPCR reactions; coupled with DNA polymerase I for DNA labeling via nick translation; and DNA fragment library construction.


Usage Guidelines:

This product is formulated for protein extraction experiments and serves as a reference guide.

  • Reaction System: Incorporate the DNase I storage solution into the protein extraction solution at a 1/100 volume ratio (resulting in a final concentration of 20 U/mL). Supplement with 1 M MgCl2 at a 1/100 volume ratio.
  • Reaction Conditions: Maintain a temperature of 37°C for a duration of 30-60 minutes. Subsequently, proceed with the planned protein extraction experiments.
Note: Eliminate EDTA from the initial protein lysate, as it chelates the Ca2+ and Mg2+ ions essential for enzyme activity, potentially reducing the digestion capacity of DNase I.