GMP DNase I: The Purest and Most Reliable DNase for Your Research Needs

Product Description:

Deoxyribonuclease I (DNase I), also known as Deoxyribonuclease I, is a nucleic acid enzyme present in various cells and tissues. It is an endonuclease that cleaves phosphodiester bonds adjacent to pyrimidines, generating polynucleotides with a 5' phosphate group and a 3' hydroxyl group. The average digestion product size is a minimum of tetranucleotides. DNase I can catalyze various forms of DNA, including single-stranded DNA, double-stranded DNA, and even chromatin (its cutting rate is influenced by histones). The optimal working range is pH 7-8. DNase activity depends on Ca2+ and can be activated by divalent metal ions such as Co2+, Mn2+, and Zn2+. 5 mM Ca2+ can protect the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cleave at any site on either strand of DNA, while in the presence of Mn2+, it can simultaneously recognize both strands of DNA and cleave at almost the same sites. DNase I was originally isolated from the pancreas and remains one of the major sources of this enzyme in mammals.

Product Components:

Product Introduction:

DNase I, a versatile nuclease, exhibits the capability to digest both single-stranded and double-stranded DNA, yielding monodeoxynucleotides or single/double-stranded oligodeoxynucleotides. It efficiently hydrolyzes phosphodiester bonds, generating monodeoxynucleotides and oligodeoxynucleotides with 5'-phosphate groups and 3'-OH groups, with the average digestion product size being the smallest tetranucleotide. Catalyzing various forms of DNA, including single-stranded DNA, double-stranded DNA, and chromatin (its cutting rate influenced by histones), DNase I operates optimally within a pH range of 7-8. Its activity is contingent on Ca2+ and can be activated by divalent metal ions like Co2+, Mn2+, Zn2+, etc. The presence of 5mm Ca2+ shields the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cut at any site on any DNA strand; in the presence of Mn2+, it can simultaneously recognize both DNA strands and cut at nearly the same position, forming blunt ends or sticky ends with 1-2 nucleotides protruding.

Product Feature:

• DNase I can typically be inactivated using heat-based methods. In scenarios necessitating the removal of residual denatured proteins, phenol-chloroform extraction is employed to precipitate RNA samples post-digestion at 37°C (without heating operation).
• GMP-compliant production system.

Product Application:

• DNase I finds widespread utility in RNA preparation devoid of DNA; removal of template DNA post in vitro transcription; preparation of DNA-free RNA before RT-PCR and RT-qPCR reactions; coupled with DNA polymerase I for DNA labeling via nick translation; and DNA fragment library construction.