Proteinase K ex. Pichia Pastoris (Type E - Recombinant) for Molecular Biology & PCR: Unleash the Power of Efficient Protein Digestion
Elevate your molecular biology and PCR workflows with Proteinase K ex. Pichia Pastoris (Type E - Recombinant), a highly active and thermostable protease designed to deliver exceptional protein digestion performance. Experience the convenience of this recombinant protease, optimized for a wide range of applications, including protein inactivation, nucleic acid purification, and tissue lysis.
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) for Molecular Biology & PCR: A Powerful Protease for Diverse Applications
In the realm of molecular biology and PCR, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has emerged as a highly versatile and effective protease, renowned for its exceptional protein digestion capabilities. This recombinant protease, derived from the yeast Pichia pastoris, offers a range of advantages that make it an invaluable tool for researchers and scientists alike.
Unparalleled Activity and Thermostability
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) exhibits remarkable catalytic activity, efficiently digesting a broad spectrum of proteins, including histones, nucleases, and immunoglobulins. Its high thermostability allows it to function effectively over a wide temperature range, ensuring optimal performance under varied conditions.
Applications in Molecular Biology and PCR
The versatility of Proteinase K ex. Pichia Pastoris (Type E - Recombinant) extends to a variety of molecular biology and PCR applications, including:
- Protein Inactivation: This protease effectively inactivates enzymes, such as DNases and RNases, preventing unwanted degradation of nucleic acids during DNA and RNA isolation procedures.
- Nucleic Acid Purification: Proteinase K is commonly employed in the purification of DNA and RNA, removing contaminating proteins that can interfere with downstream applications, such as PCR and sequencing.
- Tissue Lysis: This protease efficiently lyses tissues and cells, breaking down cellular components and releasing DNA and RNA for further analysis.
Optimized for a Wide Range of Uses
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is specifically optimized for a broad range of uses, including:
- DNA Extraction from Various Sources: It is suitable for extracting DNA from diverse sources, including animal tissues, plant tissues, and bacterial cultures.
- RNA Purification from Diverse Samples: It can effectively purify RNA from various samples, including tissues, cells, and viruses.
- Protein Digestion for Mass Spectrometry: It is used for protein digestion prior to mass spectrometry analysis, providing peptide fragments for protein identification.
High Purity and Consistency
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is produced using recombinant technology, ensuring high purity and consistency in each batch. This consistent quality control guarantees reliable and reproducible results in various molecular biology and PCR applications.
Safety Considerations
While Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is a valuable tool, it is important to handle it with care as it is a proteolytic enzyme and may cause skin and eye irritation. When working with this protease, it is recommended to wear appropriate personal protective equipment, including gloves, goggles, and a lab coat.
Storage and Stability
Proper storage and handling are crucial to maintaining the activity and stability of Proteinase K ex. Pichia Pastoris (Type E - Recombinant). This protease should be stored at -20°C to ensure optimal stability. Under proper storage conditions, it remains active for several years.
Conclusion
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has established itself as an indispensable tool in the field of molecular biology and PCR. Its exceptional activity, thermostability, and versatility make it an ideal choice for a wide range of applications, including protein inactivation, nucleic acid purification, and tissue lysis. With its high purity, consistent performance, and ease of use, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) continues to empower researchers and scientists in their pursuit of genetic insights.
Property | Specification |
---|---|
Appearance (Colour) | White |
Appearance (Form) | Crystalline Powder |
Activity | min. 40 units/mg protein |
Protein Content | min. 70% |
Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteus reagent). |
Enzyme Solution for Activity Determination | Prepared in cold 10 mM sodium acetate buffer, pH 7.5. |
Advantages of Proteinase K ex. Pichia Pastoris (Type E - Recombinant)
Compared to other proteases commonly used in molecular biology, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) offers several distinct advantages:
- High Activity: It exhibits significantly higher activity than other proteases, such as trypsin and pronase, resulting in faster and more efficient protein digestion.
- Broad Substrate Specificity: It can digest a wide range of proteins, including both basic and acidic proteins, making it a versatile tool for various applications.
- Thermostability: It remains active over a wide temperature range, allowing for efficient protein digestion at elevated temperatures, which can accelerate certain protocols.
- High Purity: Recombinant production ensures high purity and consistency, minimizing potential contamination and interference with downstream applications.
- Low Cost: It is generally more cost-effective than other proteases, making it a practical choice for routine molecular biology and PCR applications.
Applications Beyond Molecular Biology and PCR
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has also found applications in various fields beyond molecular biology and PCR, including:
- Forensic Science: It is used to digest proteins in biological samples, such as blood, tissue, and hair, for DNA analysis and identification.
- Histology: It is employed to remove proteins from tissue sections, enhancing the visibility of cellular structures during microscopic examination.
- Enzyme-Linked Immunosorbent Assay (ELISA): It is used to remove proteins from ELISA plates, reducing background noise and improving assay sensitivity.
- Protein Synthesis: It is utilized to digest proteins prior to amino acid analysis, providing valuable insights into protein structure and composition.