Proteinase K ex. Pichia Pastoris (Type E - Recombinant) for Molecular Biology & PCR: Unleash the Power of Efficient Protein Digestion
Elevate your molecular biology and PCR workflows with Proteinase K ex. Pichia Pastoris (Type E - Recombinant), a highly active and thermostable protease designed to deliver exceptional protein digestion performance. Experience the convenience of this recombinant protease, optimized for a wide range of applications, including protein inactivation, nucleic acid purification, and tissue lysis.
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) for Molecular Biology & PCR: A Powerful Protease for Diverse Applications
In the realm of molecular biology and PCR, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has emerged as a highly versatile and effective protease, renowned for its exceptional protein digestion capabilities. This recombinant protease, derived from the yeast Pichia pastoris, offers a range of advantages that make it an invaluable tool for researchers and scientists alike.
Unparalleled Activity and Thermostability
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) exhibits remarkable catalytic activity, efficiently digesting a broad spectrum of proteins, including histones, nucleases, and immunoglobulins. Its high thermostability allows it to function effectively over a wide temperature range, ensuring optimal performance under varied conditions.
Applications in Molecular Biology and PCR
The versatility of Proteinase K ex. Pichia Pastoris (Type E - Recombinant) extends to a variety of molecular biology and PCR applications, including:
- Protein Inactivation: This protease effectively inactivates enzymes, such as DNases and RNases, preventing unwanted degradation of nucleic acids during DNA and RNA isolation procedures.
- Nucleic Acid Purification: Proteinase K is commonly employed in the purification of DNA and RNA, removing contaminating proteins that can interfere with downstream applications, such as PCR and sequencing.
- Tissue Lysis: This protease efficiently lyses tissues and cells, breaking down cellular components and releasing DNA and RNA for further analysis.
Optimized for a Wide Range of Uses
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is specifically optimized for a broad range of uses, including:
- DNA Extraction from Various Sources: It is suitable for extracting DNA from diverse sources, including animal tissues, plant tissues, and bacterial cultures.
- RNA Purification from Diverse Samples: It can effectively purify RNA from various samples, including tissues, cells, and viruses.
- Protein Digestion for Mass Spectrometry: It is used for protein digestion prior to mass spectrometry analysis, providing peptide fragments for protein identification.
High Purity and Consistency
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is produced using recombinant technology, ensuring high purity and consistency in each batch. This consistent quality control guarantees reliable and reproducible results in various molecular biology and PCR applications.
While Proteinase K ex. Pichia Pastoris (Type E - Recombinant) is a valuable tool, it is important to handle it with care as it is a proteolytic enzyme and may cause skin and eye irritation. When working with this protease, it is recommended to wear appropriate personal protective equipment, including gloves, goggles, and a lab coat.
Storage and Stability
Proper storage and handling are crucial to maintaining the activity and stability of Proteinase K ex. Pichia Pastoris (Type E - Recombinant). This protease should be stored at -20°C to ensure optimal stability. Under proper storage conditions, it remains active for several years.
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has established itself as an indispensable tool in the field of molecular biology and PCR. Its exceptional activity, thermostability, and versatility make it an ideal choice for a wide range of applications, including protein inactivation, nucleic acid purification, and tissue lysis. With its high purity, consistent performance, and ease of use, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) continues to empower researchers and scientists in their pursuit of genetic insights.
|Appearance (Form)||Crystalline Powder|
|Activity||min. 40 units/mg protein|
|Protein Content||min. 70%|
|Unit Definition||One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteus reagent).|
|Enzyme Solution for Activity Determination||Prepared in cold 10 mM sodium acetate buffer, pH 7.5.|
Compared to other proteases commonly used in molecular biology, Proteinase K ex. Pichia Pastoris (Type E - Recombinant) offers several distinct advantages:
- High Activity: It exhibits significantly higher activity than other proteases, such as trypsin and pronase, resulting in faster and more efficient protein digestion.
- Broad Substrate Specificity: It can digest a wide range of proteins, including both basic and acidic proteins, making it a versatile tool for various applications.
- Thermostability: It remains active over a wide temperature range, allowing for efficient protein digestion at elevated temperatures, which can accelerate certain protocols.
- High Purity: Recombinant production ensures high purity and consistency, minimizing potential contamination and interference with downstream applications.
- Low Cost: It is generally more cost-effective than other proteases, making it a practical choice for routine molecular biology and PCR applications.
Applications Beyond Molecular Biology and PCR
Proteinase K ex. Pichia Pastoris (Type E - Recombinant) has also found applications in various fields beyond molecular biology and PCR, including:
- Forensic Science: It is used to digest proteins in biological samples, such as blood, tissue, and hair, for DNA analysis and identification.
- Histology: It is employed to remove proteins from tissue sections, enhancing the visibility of cellular structures during microscopic examination.
- Enzyme-Linked Immunosorbent Assay (ELISA): It is used to remove proteins from ELISA plates, reducing background noise and improving assay sensitivity.
- Protein Synthesis: It is utilized to digest proteins prior to amino acid analysis, providing valuable insights into protein structure and composition.