GMP Vaccinia Capping Enzyme: The Purest and Most Reliable Capping Enzyme for Your RNA Research

GMP Vaccinia Capping Enzyme: The Essential Tool for RNA Research

GMP Vaccinia Capping Enzyme is a highly purified enzyme that catalyzes the addition of a cap structure to the 5' end of RNA. Cap structures are important for RNA stability, translation, and splicing. GMP Vaccinia Capping Enzyme is produced under the strictest Good Manufacturing Practices (GMP) guidelines, ensuring the highest quality and purity.

GMP Vaccinia Capping Enzyme offers a number of benefits for RNA research, including:

• High purity and reliability: GMP Vaccinia Capping Enzyme is the purest and most reliable capping enzyme available, ensuring consistent and reproducible results.
• High efficiency and specificity: GMP Vaccinia Capping Enzyme can completely cap RNA transcripts in minutes, without damaging other biomolecules.
• Versatility: GMP Vaccinia Capping Enzyme is compatible with a wide range of buffers and reagents, making it ideal for use in a variety of RNA research applications.

GMP Vaccinia Capping Enzyme is used in a wide range of RNA research applications, including:

• mRNA vaccine production: GMP Vaccinia Capping Enzyme is used to cap mRNA transcripts during the production of mRNA vaccines. This ensures that the mRNA transcripts are stable and can be efficiently translated into proteins by the body.
• In vitro transcription: GMP Vaccinia Capping Enzyme is used to cap RNA transcripts during in vitro transcription reactions. This ensures that the RNA transcripts are stable and can be used for downstream applications, such as RNA sequencing and protein expression.
• RNA probe preparation: GMP Vaccinia Capping Enzyme is used to cap RNA probes during RNA probe preparation. This ensures that the RNA probes are stable and can be used accurately to detect and quantify specific RNA targets.
• RNA sequencing: GMP Vaccinia Capping Enzyme is used to cap RNA transcripts during RNA sequencing library preparation. This ensures that the RNA transcripts are stable and can be efficiently sequenced.
• Proteomics: GMP Vaccinia Capping Enzyme is used to cap RNA transcripts during proteomics workflows, such as ribosome profiling and RNA-protein interaction studies. This ensures that the RNA transcripts are stable and can be accurately analyzed.

Order your GMP Vaccinia Capping Enzyme today and experience the difference!

Revolutionize mRNA Modification: Vaccinia Capping Enzyme (GMP-grade)

Embark on a journey into the intricacies of mRNA modification with our Vaccinia Capping Enzyme, a GMP-grade marvel in the realm of molecular biology. In the eukaryotic realm, mRNA undergoes post-transcriptional modifications, culminating in the creation of a unique cap structure at its 5' end. This cap structure plays a pivotal role in mRNA stability, transport, and translation.

At the heart of this transformative process is the Vaccinia virus capping enzyme, a potent catalyst for cap structure formation. Comprising two subunits, D1 and D12, this enzyme exhibits a versatile skill set, boasting RNA triphosphatase activity, guanylyltransferase activity, and guanine methyltransferase activity. It adeptly connects the 7-methylguanosine cap structure (m7Gppp) to the 5' end of RNA (m7Gppp5'N) in a meticulous dance of biochemical precision.

In just an hour, our Vaccinia virus capping enzyme orchestrates the capping of RNA under optimal conditions, leveraging the right concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), and more, ensuring the correct direction for subsequent molecular processes.

Key Attributes:

• High Accuracy and Efficiency: Witness unparalleled precision in capping reactions, ensuring the fidelity of downstream processes.
• Stability Redefined: Our capping enzyme stands as a beacon of stability, providing reliability in your experiments.
• GMP-Compliant Production: Crafted with utmost adherence to GMP standards, guaranteeing a product of the highest quality.

Applications Unleashed:

• Capping Reactions: Illuminate mRNA before in vivo/in vitro translation, unraveling new dimensions in biological exploration.
• 5' End Labeling: Initiate labeling reactions of mRNA with finesse, expanding the possibilities of your molecular pursuits.

Immerse yourself in the excellence of our Vaccinia Capping Enzyme, where precision meets innovation in the dynamic landscape of molecular biology.