Bst DNA Polymerase: The Epitome of Precision and Efficiency in DNA Amplification  

Elevate your molecular biology endeavors to new heights with Bst DNA Polymerase, an exceptional enzyme renowned for its remarkable accuracy, efficiency, and versatility. Experience the pinnacle of PCR fidelity with Bst DNA Polymerase, enabling the error-free amplification of even the most challenging DNA sequences. Streamline your downstream applications with blunt-ended PCR products and witness the remarkable performance of Bst DNA Polymerase across a wide range of molecular biology applications.

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Unveiling the Power of Bst DNA Polymerase

Derived from the thermophilic bacterium Bacillus stearothermophilus, Bst DNA Polymerase emerges as a remarkable enzyme, meticulously crafted to redefine the standards of precision and efficiency in DNA amplification. Its exceptional fidelity, resulting from its robust 3'-5' exonuclease activity, meticulously scrutinizes newly synthesized DNA, correcting any misincorporations and ensuring the highest level of accuracy.

Achieving Unparalleled Fidelity for Error-Free Amplification

Bst DNA Polymerase boasts an error rate of approximately 1.3 x 10^-5, significantly lower than that of Taq DNA Polymerase. This remarkable fidelity translates into error-free amplification of even the most challenging templates, making Bst DNA the ideal choice for PCR applications where accuracy is paramount.

Amplification Efficiency: Every Target Molecule Counts

Alongside its exceptional fidelity, Bst DNA Polymerase excels in amplification efficiency. Its remarkable speed of 1 kb/10s ensures rapid PCR reactions, enabling the efficient amplification of target DNA sequences up to 6 kb in length. Whether you're amplifying short or long DNA fragments, Bst DNA Polymerase consistently delivers high-yield amplifications, ensuring that every target molecule is captured and amplified with precision.

Streamlined Downstream Applications

A notable feature of Bst DNA Polymerase is its ability to generate blunt-ended DNA products. This eliminates the need for additional enzymatic modification prior to downstream applications, such as cloning and sequencing. With blunt-ended PCR products, you can seamlessly ligate DNA fragments into vectors and directly proceed with sequencing without the hassle of overhang modification.

A Versatile Tool for Diverse Applications

Bst DNA Polymerase finds wide-ranging applications in molecular biology, from gene cloning and mutation analysis to sequencing and diagnostic PCR. Its exceptional fidelity, efficiency, and convenience make it an indispensable tool for researchers seeking the highest level of accuracy in their PCR experiments.

A Paradigm Shift in DNA Amplification

Bst DNA Polymerase stands as a testament to scientific innovation, offering unparalleled accuracy, efficiency, and convenience in DNA amplification. With its ability to generate error-free amplification of even the most challenging targets and streamline downstream applications, Bst DNA Polymerase empowers researchers to tackle a wide range of molecular biology applications with confidence. As the epitome of precision in PCR, Bst DNA Polymerase is poised to revolutionize the field of molecular biology, paving the way for groundbreaking discoveries.

Embrace the power of Bst DNA Polymerase and redefine the standards of precision and efficiency in your molecular biology endeavors.

Product Manual

Product NameBst DNA Polymerase
SourceRecombinant expression in Escherichia coli
Catalog NumberCSB-DEM074
Physical FormLiquid
Enzyme Activity8 U/μL
Storage Conditions-20 ±5°C
Storage Buffer10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Tween20, 0.5% NP-40, 50% Glycerol
Activity Definition1 U refers to the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble precipitate under conditions of 65°C for 30 minutes.
Quality ControlNo residual nucleases and exonucleases
Shelf Life24 months

Bst DNA Polymerase: Revolutionizing Isothermal Amplification with Exceptional Stability and Efficiency

Product Introduction

In the realm of molecular biology, isothermal amplification techniques have emerged as powerful alternatives to conventional PCR, offering rapid and efficient DNA amplification under constant temperature conditions. Bst DNA Polymerase stands as a beacon of innovation in this field, meticulously crafted to deliver exceptional performance in isothermal amplification. This remarkable enzyme, derived from the thermophilic bacterium Bacillus stearothermophilus, exhibits remarkable thermal stability, strand displacement activity, and polymerase activity, enabling it to amplify DNA templates with unparalleled speed, specificity, and efficiency.

Product Features: A Testament to Innovation
  • Exceptional Thermal Stability: Bst DNA Polymerase boasts remarkable thermal stability, ensuring consistent and reliable performance even at elevated temperatures. This feature makes it an ideal choice for isothermal amplification reactions, eliminating the need for complex thermal cycling protocols.
  • Robust Strand Displacement Activity: Bst DNA Polymerase possesses strong strand displacement activity, enabling it to efficiently amplify DNA templates by displacing complementary strands during polymerization. This unique property contributes to the enzyme's exceptional amplification efficiency.
  • Unparalleled Polymerase Activity: Bst DNA Polymerase exhibits exceptional polymerase activity, ensuring the rapid synthesis of DNA strands during isothermal amplification reactions. Its high processivity minimizes the likelihood of premature termination, leading to high-yield amplification of target sequences.

Product Applications: Unleashing the Power of Isothermal Amplification

Bst DNA Polymerase finds its primary application in isothermal amplification techniques, including:

  • LAMP (Loop-mediated Isothermal Amplification): Bst DNA Polymerase is the core enzyme for LAMP, a highly sensitive and specific isothermal amplification method widely used for pathogen detection and genetic analysis.
  • HNA (Helicase-mediated Isothermal Amplification): Bst DNA Polymerase plays a crucial role in HNA, an isothermal amplification method that utilizes helicase enzymes to unwind DNA templates, enabling rapid and efficient amplification.
  • NASBA (Nucleic Acid Sequence-Based Amplification): Bst DNA Polymerase serves as a key component in NASBA, an isothermal amplification technique that specifically amplifies RNA targets, making it valuable for viral and gene expression studies.
A Paradigm Shift in Isothermal Amplification

Bst DNA Polymerase represents a significant leap forward in isothermal amplification technology, offering exceptional stability, specificity, and efficiency. Its ability to amplify DNA templates under constant temperature conditions streamlines experimental protocols, reduces turnaround times, and enhances overall amplification efficiency. By empowering researchers to tackle a wide range of molecular biology applications with unparalleled precision and speed, Bst DNA Polymerase is poised to revolutionize the field of isothermal amplification, paving the way for groundbreaking discoveries and advancements.

Embrace the power of Bst DNA Polymerase and redefine the boundaries of isothermal amplification in your molecular biology endeavors.

Product Description

Experience unparalleled performance with Bst DNA Polymerase, meticulously crafted from Thermophilic Geobacillus sp DNA Polymerase I. Engineered through genetic modifications, it boasts robust 5'-3' DNA polymerase activity, strand displacement activity, and remarkable dUTP tolerance. Notably, the 5'-3' exonuclease activity has been removed, ensuring precision in amplification.

This versatile product is specifically designed for contamination-free isothermal amplification reactions, including LAMP, CPA, RCA, and various other isothermal applications. With a convenient heat inactivation feature at 85°C for 5 minutes, Bst DNA Polymerase provides a reliable and efficient solution for your amplification needs.

Product Components
LabelComponent NameSpecifications
15× Bst buffer0.6 mL, 3 mL, 6 mL
2Bst DNA Polymerase100 U, 500 U, 1000 U