WSE-7220 EzFastBlot HMW: A Powerful Transfer Buffer for High Molecular Weight Proteins

WSE-7220 EzFastBlot HMW is a high-speed transfer buffer that is specifically designed to transfer large proteins (>200kDa) quickly and efficiently. It is a methanol-free, acid-free, and detergent-free buffer that can be used with a variety of gels and membranes. WSE-7220 EzFastBlot HMW is an ideal choice for researchers who need to transfer high molecular weight proteins for Western blotting, Northern blotting, and other protein blotting applications.

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WSE-7220 EzFastBlot HMW  

WSE-7220 EzFastBlot HMW is a high molecular weight protein transfer buffer that is designed to transfer proteins up to 500 kDa in size. It is a methanol-free, acid-free, and detergent-free buffer that can be used with a variety of gels and membranes. WSE-7220 EzFastBlot HMW is a fast and efficient buffer that can be used to transfer proteins in as little as 30 minutes.  

Purpose and Application:

Speedy Transfer for High Molecular Weight Proteins (200 kDa <)

The innovative solution is tailored for the rapid and efficient transfer of high molecular weight proteins, specifically those exceeding 200 kDa. Noteworthy for its effectiveness, this method ensures swift blotting without the need for Methanol (MeOH), Acid, or Detergents.


  1. Effective Transfer of High Molecules (Up to MW 500 kDa): Achieve reliable and efficient transfer of molecules with high molecular weights, extending up to 500 kDa, ensuring accurate representation on membranes.
  2. Time Efficiency (30-60 minutes): Experience greater time shortening in blotting procedures, with a remarkably fast completion time ranging between 30 to 60 minutes.
  3. 5x Concentrated Reagent: The reagent comes in a 5x concentrated form, allowing for easy dilution with distilled water before use, providing flexibility and cost-effectiveness.
  4. MeOH, Acid, Detergent Free: Eliminate the need for Methanol, Acid, and Detergents in the blotting process, contributing to a cleaner and safer experimental environment.
  5. Long-Term Stability: The reagent boasts stability for up to one year at room temperature when stored in a dark environment, ensuring consistent performance over an extended period.

Safety Precautions

Before using the product, read this manual thoroughly at first. Do not start the operation until you understand the contents of the manual.

This document explains only methods utilized for specified purposes. Do not use the product for any purpose or by any method not described in the manual. If it is used for any purpose or by any method not described in the manual, an operator should take responsibility for all required safety measures and contingencies.

Also, read the manual of the equipment used with it simultaneously.

Additional Safety Precautions:

  • Always wear appropriate personal protective equipment (PPE) when using the product. This includes gloves, safety glasses, and a lab coat.
  • Work in a well-ventilated area.
  • Avoid contact with eyes, skin, and clothing.
  • In case of contact with eyes, flush immediately with plenty of water for at least 15 minutes.
  • In case of contact with skin, wash immediately with soap and water.
  • In case of inhalation, move to fresh air and seek medical attention if necessary.
  • In case of ingestion, do not induce vomiting and seek medical attention immediately.
  • Do not dispose of the product in the environment.
  • Store the product in a cool, dry place.
  • Keep the product out of reach of children.

Application Purpose:

The product serves as a specialized buffer designed for the purpose of transferring proteins from a gel to a membrane through the semi-blotting method following the process of electrophoresis.

Key Highlights:

  1. Efficient Protein Transfer: Facilitates the seamless transfer of proteins, ensuring their relocation from the gel to a membrane with precision and efficiency.
  2. Tailored for Semi-Blotting Method: Specifically formulated for use in the semi-blotting method, providing an effective and controlled approach to protein transfer.
  3. Post-Electrophoresis Application: Designed to be employed in the critical step of transferring proteins after electrophoresis, contributing to the overall success of the experimental process.
  4. Optimal Buffer Composition: The buffer is meticulously composed to offer an optimal environment for the successful migration and binding of proteins during the semi-blotting process.
  5. Enhanced Experimental Workflow: Incorporating this buffer into your experimental workflow ensures a streamlined and reliable process for the transfer of proteins, promoting accurate and reproducible results.


Product nameVolumePackage
EzFastBlot HMW500mL1 bottle


NameMajor Components
EzFastBlot HMWTris(Hydroxymethyl) aminomethane (5x stock solution)


Product Compliance Assurance:

Our product has been meticulously formulated to strictly adhere to regulatory standards, ensuring full compliance with relevant laws, including the PRTR Law, Poisonous and Deleterious Substances Control Act, and Industrial Safety and Health Law.

Key Points:

  1. Notifiable Material Exclusion: The product is free from any notifiable material that surpasses the regulated amounts specified by the PRTR Law. This commitment ensures the safety and compliance of our product.
  2. Adherence to Regulations: Stringent adherence to the Poisonous and Deleterious Substances Control Act guarantees that the product is manufactured and delivered in accordance with legal requirements, prioritizing the safety of users.
  3. Industrial Safety and Health Law Compliance: Our product meets the standards outlined by the Industrial Safety and Health Law, emphasizing the elimination or reduction of health and safety risks associated with its usage.
  4. Transparent Compliance Measures: Our commitment to transparency is reflected in our assurance that the product complies with the stipulated exclusion levels set by relevant laws. Users can trust in the safety and integrity of the product.
  5. Quality Assurance: Rigorous quality control measures have been implemented throughout the manufacturing process to guarantee that the product meets or exceeds industry standards and legal requirements.

Preservation Method

To ensure optimal performance and longevity of EzFastBlot HMW, follow these preservation guidelines:

  1. Unopened EzFastBlot HMW: Store unopened EzFastBlot HMW at room temperature. It maintains its stability and effectiveness until the expiration date indicated on the product label.
  2. Working Solution: While the working solution can also be stored at room temperature, it is recommended to use it as soon as possible after preparation. This will ensure optimal transfer efficiency and prevent potential degradation of the buffer components.

By adhering to these preservation measures, you can maximize the lifespan of EzFastBlot HMW and consistently achieve reliable protein transfer results.

Proper Disposal Guidelines:

To ensure environmentally responsible and safe disposal of our product, please adhere to the following guidelines:

  1. Follow Organizational Protocols: Abide by the disposal methods and procedures established by your organization. These guidelines may include specific steps for waste management to align with organizational and environmental standards.
  2. Material Composition:
    • Main Body: The main body of the bottle is composed of high-density polyethylene.
    • Lid: The lid is made of polypropylene.
  3. Recycling Consideration: Given the recyclable nature of high-density polyethylene and polypropylene, consider recycling as a preferred disposal option. Consult local recycling facilities to ensure proper recycling practices are followed.
  4. Local Regulations: Be aware of and comply with local regulations and guidelines regarding the disposal of plastic materials. Different regions may have specific requirements for waste disposal.
  5. Avoid Incineration: Refrain from incinerating the product. Incineration can release potentially harmful substances into the environment and is not recommended for plastic materials.
  6. Contact Authorities: If you have any uncertainties or if your organization lacks specific disposal guidelines, contact local waste management authorities for guidance on the appropriate disposal methods.


A. Diluting the Reagent

To prepare the working solution (blotting buffer), dilute the EzFastBlot HMW product to 1/5 of its original concentration using distilled water. This dilution step is crucial for achieving optimal protein transfer efficiency.

B. Making a PVDF Membrane Hydrophilic

If using a PVDF membrane for blotting, it is essential to render it hydrophilic beforehand to ensure proper protein binding. The following method effectively hydrates the membrane:

  1. Fill a container slightly larger than the PVDF membrane with methanol.
  2. Submerge the membrane in the methanol for approximately 10 seconds.

C. Blotting Procedure

  1. Preparing Filter Papers:b. If using filter papers from a different manufacturer, ensure they are cut to the same size as the gel.c. Prepare enough filter papers to achieve a total thickness of at least 6 mm.d. Pour approximately 50 mL of blotting buffer into a container larger than the filter papers and submerge them in the solution.
  2. Post-Electrophoresis Gel Preparation:b. Gently rinse the gel surface for a few minutes to remove any remaining gel fragments or SDS bubbles. Avoid prolonged soaking or shaking.