A. General methods for preparing gradients; preparing crude organelle or membrane

fractions and analysing the gradient separation

B. An alphabetical subcellular membrane index. Membranes and organelles from

tissues and cells from mammals and most higher eukaryotes are listed according to the

membrane or organelle or subcellular process (e.g. endocytosis).

C. Membranes and organelles from protozoa, fungi, algae, plants etc are listed

alphabetically according to the source.

♦ Click on the relevant [APPLICATION SHEET] for a detailed protocol. In some cases

more than one Application Sheet for a specific membrane type may be provided if

different practical strategies are available.