Purification of bacteria and bacterial minicells from cultured cells
Although Nycodenz has been widely used in the purification of a broad range of bacteria from soil samples (and other biological matrices), see Application Sheet C39, there are in addition some other specific examples of the purification of bacteria, which have been primarily grown in either mammalian or non-mammalian cells (or organisms), using both Nycodenz and OptiPrep.
Some of these methods are summarized in this Application Sheet. The majority of the published methodology is concerned with obligate intracellular bacteria and two of these, Chlamydophila abortus and Piscirickettsia salmonis are considered in Sections 2 and 3.
Sections 4-10 summarize some of the other
important applications of OptiPrep for a broad range of other bacteria.