Types of centrifugal separations
One way of improving the resolution of particles by size is to minimize the difference in RCF between the top and bottom of the sample. Practically this is achieved by layering the sample as a narrow band over a continuous density gradient.
This is called rate-zonal gradient (sedimentation velocity) centrifugation; the conditions are chosen such that ρρ always remains greater than ρl . In a continuous gradient the density increases in a smooth but not necessarily linear fashion and can be generated by a number of methods described later. By allowing the particles to sediment through such a density gradient the resolution and recovery of particles is generally improved.
The particles move down through the gradient in the
form of discrete zones at a rate that depends principally
on their size (see figure below). Resolution between the
zones decreases as the radial thickness of the sample
increases so the efficacy of rate zonal fractionation is
limited by sample size, which is commonly no more
than 10% of the total gradient volume.